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Improvement of biodiesel production using waste cooking oil and applying single and mixed immobilised lipases on polyhydroxyalkanoate

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  • Binhayeeding, Narisa
  • Klomklao, Sappasith
  • Prasertsan, Poonsuk
  • Sangkharak, Kanokphorn

Abstract

This study describes the production of biodiesel via the enzymatic transesterification reaction, using waste cooking oil as a substrate. Lipases from Candida rugosa and Rhizomucor miehei were immobilised on polyhydroxybutyrate to serve as environment-friendly catalysts. This enzyme mixture yielded the highest biodiesel production. The optimum conditions for biodiesel production included a reaction temperature of 45 °C, 5% water content, 1% of mixed lipase (50% of each lipase), a methanol:oil ratio of 6:1, a 24-h reaction period, and a 250 rpm stirring speed, which resulted in the highest biodiesel yields (96.5%). This catalyst can be reused for more than six cycles, after which the lipase activity begins to decrease. The chemical and fuel properties such as the density at 15 °C, viscosity at 40 °C and cloud point of the resulting biodiesel were similar to those of other diesels/biodiesels and complied with international specifications (EN 14214 and ASTM D 6751).

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  • Binhayeeding, Narisa & Klomklao, Sappasith & Prasertsan, Poonsuk & Sangkharak, Kanokphorn, 2020. "Improvement of biodiesel production using waste cooking oil and applying single and mixed immobilised lipases on polyhydroxyalkanoate," Renewable Energy, Elsevier, vol. 162(C), pages 1819-1827.
  • Handle: RePEc:eee:renene:v:162:y:2020:i:c:p:1819-1827
    DOI: 10.1016/j.renene.2020.10.009
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    11. Zhang, Pingbo & Liu, Peng & Fan, Mingming & Jiang, Pingping & Haryono, Agus, 2021. "High-performance magnetite nanoparticles catalyst for biodiesel production: Immobilization of 12-tungstophosphoric acid on SBA-15 works effectively," Renewable Energy, Elsevier, vol. 175(C), pages 244-252.
    12. Loh, Jun Mann & Amelia, & Gourich, Wail & Chew, Chien Lye & Song, Cher Pin & Chan, Eng-Seng, 2021. "Improved biodiesel production from sludge palm oil catalyzed by a low-cost liquid lipase under low-input process conditions," Renewable Energy, Elsevier, vol. 177(C), pages 348-358.
    13. Zhang, Qiaofei & Xie, Wenlei & Li, Jiangbo & Guo, Lihong, 2023. "Bimetallic Zrx-Aly-KIT-6 modified with sulfate as acidic catalyst for biodiesel production from low-grade acidic oils," Renewable Energy, Elsevier, vol. 217(C).
    14. Vávra, Aleš & Hájek, Martin & Kocián, David, 2021. "The influence of vegetable oils composition on separation of transesterification products, especially quality of glycerol," Renewable Energy, Elsevier, vol. 176(C), pages 262-268.
    15. Verónica Ávila Vázquez & Miguel Mauricio Aguilera Flores & Luis Felipe Hernández Casas & Nahum Andrés Medellín Castillo & Alejandro Rocha Uribe & Hans Christian Correa Aguado, 2023. "Biodiesel Production Catalyzed by Lipase Extract Powder of Leonotis nepetifolia (Christmas Candlestick) Seed," Energies, MDPI, vol. 16(6), pages 1-13, March.

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