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Optimized synthesis of biodiesel using lipase from Pacific white shrimp (Litopenaeus vannamei) hepatopancreas

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  • Kuepethkaew, Sakonwat
  • Sangkharak, Kanokphorn
  • Benjakul, Soottawat
  • Klomklao, Sappasith

Abstract

Enzymatic production of biodiesel from palm oil using partially purified lipase from the hepatopancreas of Pacific white shrimp (Litopenaeus vannamei) as a low cost catalyst was studied. In this optimization study, the effects of reaction time, enzyme concentration, methanol/oil molar ratio, water content and reaction temperature on the biodiesel yield were considered. The best conditions for biodiesel preparation were: 70 kUnit lipase, 4:1 methanol to oil molar ratio, 3% water, 45 °C reaction temperature and a reaction time of 16 h. Under these optimal conditions, the maximum biodiesel yield reached 97.01%. The attenuated total reflection-Fourier transform infrared spectroscopy (ATR-FTIR) and thin layer chromatography (TLC) were used to ensure the conversion of palm oil into biodiesel. The biodiesel properties were within the recommended biodiesel standards as prescribed by EN 14214 and ASTM D 6751. Therefore, the lipase from the hepatopancreas of Pacific white shrimp is a potential biocatalyst for the biodiesel industry.

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  • Kuepethkaew, Sakonwat & Sangkharak, Kanokphorn & Benjakul, Soottawat & Klomklao, Sappasith, 2017. "Optimized synthesis of biodiesel using lipase from Pacific white shrimp (Litopenaeus vannamei) hepatopancreas," Renewable Energy, Elsevier, vol. 104(C), pages 139-147.
  • Handle: RePEc:eee:renene:v:104:y:2017:i:c:p:139-147
    DOI: 10.1016/j.renene.2016.12.014
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    References listed on IDEAS

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    1. Binhayeeding, Narisa & Klomklao, Sappasith & Prasertsan, Poonsuk & Sangkharak, Kanokphorn, 2020. "Improvement of biodiesel production using waste cooking oil and applying single and mixed immobilised lipases on polyhydroxyalkanoate," Renewable Energy, Elsevier, vol. 162(C), pages 1819-1827.
    2. Jaiswal, Krishna Kumar & Dutta, Swapnamoy & Banerjee, Ishita & Jaiswal, Km Smriti & Renuka, Nirmal & Ratha, Sachitra Kumar & Jaiswal, Amit K., 2024. "Valorization of fish processing industry waste for biodiesel production: Opportunities, challenges, and technological perspectives," Renewable Energy, Elsevier, vol. 220(C).
    3. Patchimpet, Jaran & Simpson, Benjamin K. & Sangkharak, Kanokphorn & Klomklao, Sappasith, 2020. "Optimization of process variables for the production of biodiesel by transesterification of used cooking oil using lipase from Nile tilapia viscera," Renewable Energy, Elsevier, vol. 153(C), pages 861-869.
    4. Kumar, Dilip & Das, Tapas & Giri, Balendu Shekher & Verma, Bhawna, 2020. "Preparation and characterization of novel hybrid bio-support material immobilized from Pseudomonas cepacia lipase and its application to enhance biodiesel production," Renewable Energy, Elsevier, vol. 147(P1), pages 11-24.

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