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Biodiesel Production Catalyzed by Lipase Extract Powder of Leonotis nepetifolia (Christmas Candlestick) Seed

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  • Verónica Ávila Vázquez

    (Interdisciplinary Professional Unit of Engineering, Campus Zacatecas, Instituto Politécnico Nacional, Blvd. del Bote 202 Cerro del Gato Ejido La Escondida, Col. Ciudad Administrativa, Zacatecas 98160, Mexico)

  • Miguel Mauricio Aguilera Flores

    (Interdisciplinary Professional Unit of Engineering, Campus Zacatecas, Instituto Politécnico Nacional, Blvd. del Bote 202 Cerro del Gato Ejido La Escondida, Col. Ciudad Administrativa, Zacatecas 98160, Mexico)

  • Luis Felipe Hernández Casas

    (Interdisciplinary Professional Unit of Engineering, Campus Zacatecas, Instituto Politécnico Nacional, Blvd. del Bote 202 Cerro del Gato Ejido La Escondida, Col. Ciudad Administrativa, Zacatecas 98160, Mexico)

  • Nahum Andrés Medellín Castillo

    (Graduate Studies and Research Center, Faculty of Engineering, Autonomous University of San Luis Potosi, Av. Manuel Nava No. 8, Col. Zona Universitaria Poniente, San Luis Potosí 78290, Mexico)

  • Alejandro Rocha Uribe

    (Graduate Studies and Research Center, Faculty of Chemical, Autonomous University of San Luis Potosi, Av. Manuel Nava No. 6, Col. Zona Universitaria Poniente, San Luis Potosí 78210, Mexico)

  • Hans Christian Correa Aguado

    (Interdisciplinary Professional Unit of Engineering, Campus Zacatecas, Instituto Politécnico Nacional, Blvd. del Bote 202 Cerro del Gato Ejido La Escondida, Col. Ciudad Administrativa, Zacatecas 98160, Mexico)

Abstract

This work aimed to evaluate the ability of lipase extract powder obtained from Leonotis nepetifolia seed for enzyme-catalyzed biodiesel production using Leonotis nepetifolia oil, commercial olive oil, and waste cooking oil as substrates. The lipase extract powder showed an enzymatic activity and hydrolysis percentage of 24.7 U/g and 21.31%, respectively, using commercial olive oil as a reference. Transesterification reaction conditions were 40 g of substrate, 34 °C, molar ratio oil: methanol of 1:3, lipase extract powder 20 wt%, phosphates buffer (pH 4.8) 20 wt%, and a reaction time of 8 h. Transesterification yields of 74.5%, 71.5%, and 69.3% for commercial olive oil, waste cooking oil, and Leonotis nepetifolia oil were obtained, respectively. Biodiesel physicochemical parameters were analyzed and compared with the international standards: EN 14214 (European Union) and ASTM D6751 (American Society for Testing and Materials). The biodiesel’s moisture and volatile matter percentages, iodine index, cooper strip corrosion, and methyl esters content conformed to the standards’ specifications. The fatty acid methyl ester content of the vegetable oils showed the presence of methyl oleate after enzyme-catalyzed transesterification. This study reveals that biodiesel production catalyzed by lipase extract powder from Leonotis nepetifolia could be a viable alternative, showing that transesterification yields competitive results.

Suggested Citation

  • Verónica Ávila Vázquez & Miguel Mauricio Aguilera Flores & Luis Felipe Hernández Casas & Nahum Andrés Medellín Castillo & Alejandro Rocha Uribe & Hans Christian Correa Aguado, 2023. "Biodiesel Production Catalyzed by Lipase Extract Powder of Leonotis nepetifolia (Christmas Candlestick) Seed," Energies, MDPI, vol. 16(6), pages 1-13, March.
    • Handle: RePEc:gam:jeners:v:16:y:2023:i:6:p:2848-:d:1101394
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    References listed on IDEAS

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    Cited by:

    1. Juliana Gisele Corrêa Rodrigues & Fernanda Veras Cardoso & Celine Campos dos Santos & Rosiane Rodrigues Matias & Nélio Teixeira Machado & Sergio Duvoisin Junior & Patrícia Melchionna Albuquerque, 2023. "Biocatalyzed Transesterification of Waste Cooking Oil for Biodiesel Production Using Lipase from the Amazonian Fungus Endomelanconiopsis endophytica," Energies, MDPI, vol. 16(19), pages 1-19, October.
    2. Kavitha Munisamy Sambasivam & Praveen Kuppan & Lafiya Shanavas Laila & Viswanaathan Shashirekha & Krishnamurthi Tamilarasan & Sudharsanam Abinandan, 2023. "Kernel-Based Biodiesel Production from Non-Edible Oil Seeds: Techniques, Optimization, and Environmental Implications," Energies, MDPI, vol. 16(22), pages 1-34, November.

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