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Production of biodiesel catalyzed by immobilized Pseudomonas cepacia lipase from Sapium sebiferum oil in micro-aqueous phase

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  • Li, Qin
  • Yan, Yunjun

Abstract

A new technique of biodiesel production from Sapium sebiferum oil catalyzed by immobilized lipase from Pseudomonas cepacia G63 prepared in our laboratory was investigated in this study. The independent factors were studied and the significant factors to the yield of biodeisel were confirmed, and the Box-Behnken design was employed to evaluate the effects of those significant factors on yield in preparation of biodiesel. Results indicated the optimal condition for biodiesel preparation were: 4:1 methano/oil molar ratio, 2.7% (w/w) lipase, temperature 41 °C, and the subsequent verification experiment got a result of (96.22%) kept coincided with the predicted biodiesel yield (97.07%) under the optimal conditions, and R2 = 98.19% shown the model was considered to be accurate and available for predicting the yield of biodiesel. There was no loss nearly in the immobilized lipase activity after being repeatedly used for 20 cycles at the optimal reaction condition.

Suggested Citation

  • Li, Qin & Yan, Yunjun, 2010. "Production of biodiesel catalyzed by immobilized Pseudomonas cepacia lipase from Sapium sebiferum oil in micro-aqueous phase," Applied Energy, Elsevier, vol. 87(10), pages 3148-3154, October.
    • Handle: RePEc:eee:appene:v:87:y:2010:i:10:p:3148-3154
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    15. Hu, Shengyang & Guan, Yanping & Wang, Yun & Han, Heyou, 2011. "Nano-magnetic catalyst KF/CaO-Fe3O4 for biodiesel production," Applied Energy, Elsevier, vol. 88(8), pages 2685-2690, August.
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    17. Tran, Dang-Thuan & Chen, Ching-Lung & Chang, Jo-Shu, 2016. "Continuous biodiesel conversion via enzymatic transesterification catalyzed by immobilized Burkholderia lipase in a packed-bed bioreactor," Applied Energy, Elsevier, vol. 168(C), pages 340-350.
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