IDEAS home Printed from https://ideas.repec.org/a/eee/renene/v147y2020ip1p11-24.html
   My bibliography  Save this article

Preparation and characterization of novel hybrid bio-support material immobilized from Pseudomonas cepacia lipase and its application to enhance biodiesel production

Author

Listed:
  • Kumar, Dilip
  • Das, Tapas
  • Giri, Balendu Shekher
  • Verma, Bhawna

Abstract

In the present study, hybrid bio-support catalyst (BSC) has been prepared for the production of biodiesel from Castor oil. Lipase from Pseudomonas cepacia has been immobilized on a hybrid bio-support material (BSM) by entrapment method and characterized. The highest immobilized lipase activity and loading efficiency were 87.28 (U/g) and 55.2%, respectively. The composition of Castor oil expelled from Castor seeds evaluated through Gas Chromatograph-Mass Spectrometer (GC-MS) technique. GCMS spectra show the percentage composition of ricinoleic, linoleic, oleic, stearic, palmitic, and nonanoic acid, as 78.67, 9.62, 6.65, 1.53, 2.19, and 0.08, respectively. Optimized conditions for the transesterification of Castor oil with propan-2-ol as acyl acceptor in solvent free medium were, 10% immobilized lipase relying on oil-weight, molar ratio of alcohol to oil (6:1) at 50±1°C for the period of 24 h. The product yield was estimated as 75% while the calculated conversion of biodiesel was approximately 78%. The reusability of hybrid BSC for 12 runs was found to be 70% of its initial biodiesel yield after 6 cycles. The properties of oil and biodiesel were calculated using American Standards for Testing Material (ASTM) D6751; formation of biodiesel confirmed by Proton Nuclear Magnetic Resonance (1H NMR) and Fourier Transform Infrared (FTIR) analysis.

Suggested Citation

  • Kumar, Dilip & Das, Tapas & Giri, Balendu Shekher & Verma, Bhawna, 2020. "Preparation and characterization of novel hybrid bio-support material immobilized from Pseudomonas cepacia lipase and its application to enhance biodiesel production," Renewable Energy, Elsevier, vol. 147(P1), pages 11-24.
    • Handle: RePEc:eee:renene:v:147:y:2020:i:p1:p:11-24
    DOI: 10.1016/j.renene.2019.08.110
    as

    Download full text from publisher

    File URL: http://www.sciencedirect.com/science/article/pii/S0960148119312972
    Download Restriction: Full text for ScienceDirect subscribers only
    File URL: https://libkey.io/10.1016/j.renene.2019.08.110?utm_source=ideas
    LibKey link: if access is restricted and if your library uses this service, LibKey will redirect you to where you can use your library subscription to access this item
    ---><---

    As the access to this document is restricted, you may want to search for a different version of it.

    References listed on IDEAS

    as
    1. Ji, Xi & Long, Xianling, 2016. "A review of the ecological and socioeconomic effects of biofuel and energy policy recommendations," Renewable and Sustainable Energy Reviews, Elsevier, vol. 61(C), pages 41-52.
    2. Safieddin Ardebili, M. & Ghobadian, B. & Najafi, G. & Chegeni, A., 2011. "Biodiesel production potential from edible oil seeds in Iran," Renewable and Sustainable Energy Reviews, Elsevier, vol. 15(6), pages 3041-3044, August.
    3. Atabani, A.E. & Silitonga, A.S. & Ong, H.C. & Mahlia, T.M.I. & Masjuki, H.H. & Badruddin, Irfan Anjum & Fayaz, H., 2013. "Non-edible vegetable oils: A critical evaluation of oil extraction, fatty acid compositions, biodiesel production, characteristics, engine performance and emissions production," Renewable and Sustainable Energy Reviews, Elsevier, vol. 18(C), pages 211-245.
    4. Nayak, Sheetal N. & Bhasin, Chandra Prakash & Nayak, Milap G., 2019. "A review on microwave-assisted transesterification processes using various catalytic and non-catalytic systems," Renewable Energy, Elsevier, vol. 143(C), pages 1366-1387.
    5. Kuepethkaew, Sakonwat & Sangkharak, Kanokphorn & Benjakul, Soottawat & Klomklao, Sappasith, 2017. "Optimized synthesis of biodiesel using lipase from Pacific white shrimp (Litopenaeus vannamei) hepatopancreas," Renewable Energy, Elsevier, vol. 104(C), pages 139-147.
    6. Dutta, Kasturi & Daverey, Achlesh & Lin, Jih-Gaw, 2014. "Evolution retrospective for alternative fuels: First to fourth generation," Renewable Energy, Elsevier, vol. 69(C), pages 114-122.
    7. Babaki, Mohadese & Yousefi, Maryam & Habibi, Zohreh & Mohammadi, Mehdi, 2017. "Process optimization for biodiesel production from waste cooking oil using multi-enzyme systems through response surface methodology," Renewable Energy, Elsevier, vol. 105(C), pages 465-472.
    8. Rozina, & Ahmad, Mushtaq & Zafar, Muhammad & Ali, Nasir & Lu, Houfang, 2017. "Biodiesel synthesis from Saussurea heteromalla (D.Don) Hand-Mazz integrating ethanol production using biorefinery approach," Energy, Elsevier, vol. 141(C), pages 1810-1818.
    9. Marín-Suárez, Marta & Méndez-Mateos, David & Guadix, Antonio & Guadix, Emilia M., 2019. "Reuse of immobilized lipases in the transesterification of waste fish oil for the production of biodiesel," Renewable Energy, Elsevier, vol. 140(C), pages 1-8.
    10. Murillo, Gabriel & Ali, Sameh S. & Sun, Jianzhong & Yan, Yunjun & Bartocci, Pietro & El-Zawawy, Nessma & Azab, Maha & He, Yaojia & Fantozzi, Francesco, 2019. "Ultrasonic emulsification assisted immobilized Burkholderia cepacia lipase catalyzed transesterification of soybean oil for biodiesel production in a novel reactor design," Renewable Energy, Elsevier, vol. 135(C), pages 1025-1034.
    11. Matinja, Adamu Idris & Mohd Zain, Nor Azimah & Suhaimi, Mohd Suardi & Alhassan, Adamu Jibril, 2019. "Optimization of biodiesel production from palm oil mill effluent using lipase immobilized in PVA-alginate-sulfate beads," Renewable Energy, Elsevier, vol. 135(C), pages 1178-1185.
    12. Christopher, Lew P. & Hemanathan Kumar, & Zambare, Vasudeo P., 2014. "Enzymatic biodiesel: Challenges and opportunities," Applied Energy, Elsevier, vol. 119(C), pages 497-520.
    13. Issariyakul, Titipong & Dalai, Ajay K., 2014. "Biodiesel from vegetable oils," Renewable and Sustainable Energy Reviews, Elsevier, vol. 31(C), pages 446-471.
    14. Fazal, M.A. & Haseeb, A.S.M.A. & Masjuki, H.H., 2011. "Biodiesel feasibility study: An evaluation of material compatibility; performance; emission and engine durability," Renewable and Sustainable Energy Reviews, Elsevier, vol. 15(2), pages 1314-1324, February.
    15. Government of India GOI, 2018. "National Policy on Biofuels," Working Papers id:12780, eSocialSciences.
    16. Chia, Shir Reen & Ong, Hwai Chyuan & Chew, Kit Wayne & Show, Pau Loke & Phang, Siew-Moi & Ling, Tau Chuan & Nagarajan, Dillirani & Lee, Duu-Jong & Chang, Jo-Shu, 2018. "Sustainable approaches for algae utilisation in bioenergy production," Renewable Energy, Elsevier, vol. 129(PB), pages 838-852.
    17. Rajesh Kumar, B. & Saravanan, S., 2016. "Use of higher alcohol biofuels in diesel engines: A review," Renewable and Sustainable Energy Reviews, Elsevier, vol. 60(C), pages 84-115.
    18. Shahedi, Mansour & Yousefi, Maryam & Habibi, Zohreh & Mohammadi, Mehdi & As'habi, Mohammad Ali, 2019. "Co-immobilization of Rhizomucor miehei lipase and Candida antarctica lipase B and optimization of biocatalytic biodiesel production from palm oil using response surface methodology," Renewable Energy, Elsevier, vol. 141(C), pages 847-857.
    19. Mohd Noor, C.W. & Noor, M.M. & Mamat, R., 2018. "Biodiesel as alternative fuel for marine diesel engine applications: A review," Renewable and Sustainable Energy Reviews, Elsevier, vol. 94(C), pages 127-142.
    20. Babaki, Mohadese & Yousefi, Maryam & Habibi, Zohreh & Mohammadi, Mehdi & Yousefi, Parisa & Mohammadi, Javad & Brask, Jesper, 2016. "Enzymatic production of biodiesel using lipases immobilized on silica nanoparticles as highly reusable biocatalysts: effect of water, t-butanol and blue silica gel contents," Renewable Energy, Elsevier, vol. 91(C), pages 196-206.
    21. Arumugam, A. & Ponnusami, V., 2019. "Biodiesel production from Calophyllum inophyllum oil a potential non-edible feedstock: An overview," Renewable Energy, Elsevier, vol. 131(C), pages 459-471.
    22. Jayaraman, Jayaprabakar & Alagu, Karthikeyan & Appavu, Prabhu & Joy, Nivin & Jayaram, Parthipan & Mariadoss, Anish, 2020. "Enzymatic production of biodiesel using lipase catalyst and testing of an unmodified compression ignition engine using its blends with diesel," Renewable Energy, Elsevier, vol. 145(C), pages 399-407.
    Full references (including those not matched with items on IDEAS)

    Citations

    Citations are extracted by the CitEc Project, subscribe to its RSS feed for this item.
    as


    Cited by:

    1. Al-Hwaiti, Mohammad S. & Alsbou, Eid M. & Al Haddad, Rawan M. & Osman, Ahmed I. & Jrai, Ahmed Abu & Al-Muhtaseb, Ala’a H. & Hasan, Ahmad O. & Morgan, Kevin & El-Sayed, El-Sayed M. & Al-Fatesh, Ahmed S, 2020. "Spatio-temporal analyses of extracted citrullus colocynthis seeds (Handal seed oil) as biofuel in internal combustion engine," Renewable Energy, Elsevier, vol. 166(C), pages 234-244.
    2. Wancura, João H.C. & Brondani, Michel & dos Santos, Maicon S.N. & Oro, Carolina E.D. & Wancura, Guilherme C. & Tres, Marcus V. & Oliveira, J. Vladimir, 2023. "Demystifying the enzymatic biodiesel: How lipases are contributing to its technological advances," Renewable Energy, Elsevier, vol. 216(C).
    3. Carlos S. Osorio-González & Natali Gómez-Falcon & Fabiola Sandoval-Salas & Rahul Saini & Satinder K. Brar & Antonio Avalos Ramírez, 2020. "Production of Biodiesel from Castor Oil: A Review," Energies, MDPI, vol. 13(10), pages 1-22, May.

    Most related items

    These are the items that most often cite the same works as this one and are cited by the same works as this one.
    1. Monteiro, Rodolpho R.C. & Arana-Peña, Sara & da Rocha, Thays N. & Miranda, Letícia P. & Berenguer-Murcia, Ángel & Tardioli, Paulo W. & dos Santos, José C.S. & Fernandez-Lafuente, Roberto, 2021. "Liquid lipase preparations designed for industrial production of biodiesel. Is it really an optimal solution?," Renewable Energy, Elsevier, vol. 164(C), pages 1566-1587.
    2. Tamilselvan, P. & Nallusamy, N. & Rajkumar, S., 2017. "A comprehensive review on performance, combustion and emission characteristics of biodiesel fuelled diesel engines," Renewable and Sustainable Energy Reviews, Elsevier, vol. 79(C), pages 1134-1159.
    3. Jaiswal, Krishna Kumar & Dutta, Swapnamoy & Banerjee, Ishita & Jaiswal, Km Smriti & Renuka, Nirmal & Ratha, Sachitra Kumar & Jaiswal, Amit K., 2024. "Valorization of fish processing industry waste for biodiesel production: Opportunities, challenges, and technological perspectives," Renewable Energy, Elsevier, vol. 220(C).
    4. Wancura, João H.C. & Brondani, Michel & dos Santos, Maicon S.N. & Oro, Carolina E.D. & Wancura, Guilherme C. & Tres, Marcus V. & Oliveira, J. Vladimir, 2023. "Demystifying the enzymatic biodiesel: How lipases are contributing to its technological advances," Renewable Energy, Elsevier, vol. 216(C).
    5. Sun, Shangde & Li, Kaiyue, 2020. "Biodiesel production from phoenix tree seed oil catalyzed by liquid lipozyme TL100L," Renewable Energy, Elsevier, vol. 151(C), pages 152-160.
    6. Carlos S. Osorio-González & Natali Gómez-Falcon & Fabiola Sandoval-Salas & Rahul Saini & Satinder K. Brar & Antonio Avalos Ramírez, 2020. "Production of Biodiesel from Castor Oil: A Review," Energies, MDPI, vol. 13(10), pages 1-22, May.
    7. Khozeymeh Nezhad, Marziyeh & Aghaei, Hamidreza, 2021. "Tosylated cloisite as a new heterofunctional carrier for covalent immobilization of lipase and its utilization for production of biodiesel from waste frying oil," Renewable Energy, Elsevier, vol. 164(C), pages 876-888.
    8. Yesilyurt, Murat Kadir & Cesur, Cüneyt & Aslan, Volkan & Yilbasi, Zeki, 2020. "The production of biodiesel from safflower (Carthamus tinctorius L.) oil as a potential feedstock and its usage in compression ignition engine: A comprehensive review," Renewable and Sustainable Energy Reviews, Elsevier, vol. 119(C).
    9. Sudalai, S & Rupesh, K J & Devanesan, M.G & Arumugam, A, 2023. "A critical review of Madhuca indica as an efficient biodiesel producer: Towards sustainability," Renewable and Sustainable Energy Reviews, Elsevier, vol. 188(C).
    10. Yuan, Hao & Zhang, Xinru & Jiang, Zeyi & Wang, Xinyu & Wang, Yi & Cao, Limei & Zhang, Xinxin, 2020. "Effect of light spectra on microalgal biofilm: Cell growth, photosynthetic property, and main organic composition," Renewable Energy, Elsevier, vol. 157(C), pages 83-89.
    11. Teoh, Y.H. & How, H.G. & Masjuki, H.H. & Nguyen, H.-T. & Kalam, M.A. & Alabdulkarem, A., 2019. "Investigation on particulate emissions and combustion characteristics of a common-rail diesel engine fueled with Moringa oleifera biodiesel-diesel blends," Renewable Energy, Elsevier, vol. 136(C), pages 521-534.
    12. Mahmudul, H.M. & Hagos, F.Y. & Mamat, R. & Adam, A. Abdul & Ishak, W.F.W. & Alenezi, R., 2017. "Production, characterization and performance of biodiesel as an alternative fuel in diesel engines – A review," Renewable and Sustainable Energy Reviews, Elsevier, vol. 72(C), pages 497-509.
    13. Chiong, Meng-Choung & Kang, Hooi-Siang & Shaharuddin, Nik Mohd Ridzuan & Mat, Shabudin & Quen, Lee Kee & Ten, Ki-Hong & Ong, Muk Chen, 2021. "Challenges and opportunities of marine propulsion with alternative fuels," Renewable and Sustainable Energy Reviews, Elsevier, vol. 149(C).
    14. Kumar, Praveen & Srivastava, Vimal Chandra & Jha, Mithilesh Kumar, 2016. "Jatropha curcas phytotomy and applications: Development as a potential biofuel plant through biotechnological advancements," Renewable and Sustainable Energy Reviews, Elsevier, vol. 59(C), pages 818-838.
    15. Cédric Decarpigny & Abdulhadi Aljawish & Cédric His & Bertrand Fertin & Muriel Bigan & Pascal Dhulster & Michel Millares & Rénato Froidevaux, 2022. "Bioprocesses for the Biodiesel Production from Waste Oils and Valorization of Glycerol," Energies, MDPI, vol. 15(9), pages 1-30, May.
    16. Jin, Chao & Sun, Tianyun & Ampah, Jeffrey Dankwa & Liu, Xin & Geng, Zhenlong & Afrane, Sandylove & Yusuf, Abdulfatah Abdu & Liu, Haifeng, 2022. "Comparative study on synthetic and biological surfactants’ role in phase behavior and fuel properties of marine heavy fuel oil-low carbon alcohol blends under different temperatures," Renewable Energy, Elsevier, vol. 195(C), pages 841-852.
    17. Azad, A.K. & Rasul, M.G. & Khan, M.M.K. & Sharma, Subhash C. & Mofijur, M. & Bhuiya, M.M.K., 2016. "Prospects, feedstocks and challenges of biodiesel production from beauty leaf oil and castor oil: A nonedible oil sources in Australia," Renewable and Sustainable Energy Reviews, Elsevier, vol. 61(C), pages 302-318.
    18. Carolin Nuortila & Riikka Help & Katriina Sirviö & Helena Suopanki & Sonja Heikkilä & Seppo Niemi, 2020. "Selected Fuel Properties of Alcohol and Rapeseed Oil Blends," Energies, MDPI, vol. 13(15), pages 1-11, July.
    19. Binhayeeding, Narisa & Klomklao, Sappasith & Prasertsan, Poonsuk & Sangkharak, Kanokphorn, 2020. "Improvement of biodiesel production using waste cooking oil and applying single and mixed immobilised lipases on polyhydroxyalkanoate," Renewable Energy, Elsevier, vol. 162(C), pages 1819-1827.
    20. Behdad Shadidi & Gholamhassan Najafi & Mohammad Ali Zolfigol, 2022. "A Review of the Existing Potentials in Biodiesel Production in Iran," Sustainability, MDPI, vol. 14(6), pages 1-18, March.

    Corrections

    All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:eee:renene:v:147:y:2020:i:p1:p:11-24. See general information about how to correct material in RePEc.

    If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.

    If CitEc recognized a bibliographic reference but did not link an item in RePEc to it, you can help with this form .

    If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.

    For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: Catherine Liu (email available below). General contact details of provider: http://www.journals.elsevier.com/renewable-energy .

    Please note that corrections may take a couple of weeks to filter through the various RePEc services.

    IDEAS is a RePEc service. RePEc uses bibliographic data supplied by the respective publishers.