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Assessing and advancing the safety of CRISPR-Cas tools: from DNA to RNA editing

Author

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  • Jianli Tao

    (Boston Children’s Hospital and Harvard Medical School)

  • Daniel E. Bauer

    (Harvard Medical School)

  • Roberto Chiarle

    (Boston Children’s Hospital and Harvard Medical School
    University of Torino)

Abstract

CRISPR-Cas gene editing has revolutionized experimental molecular biology over the past decade and holds great promise for the treatment of human genetic diseases. Here we review the development of CRISPR-Cas9/Cas12/Cas13 nucleases, DNA base editors, prime editors, and RNA base editors, focusing on the assessment and improvement of their editing precision and safety, pushing the limit of editing specificity and efficiency. We summarize the capabilities and limitations of each CRISPR tool from DNA editing to RNA editing, and highlight the opportunities for future improvements and applications in basic research, as well as the therapeutic and clinical considerations for their use in patients.

Suggested Citation

  • Jianli Tao & Daniel E. Bauer & Roberto Chiarle, 2023. "Assessing and advancing the safety of CRISPR-Cas tools: from DNA to RNA editing," Nature Communications, Nature, vol. 14(1), pages 1-16, December.
    • Handle: RePEc:nat:natcom:v:14:y:2023:i:1:d:10.1038_s41467-023-35886-6
    DOI: 10.1038/s41467-023-35886-6
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    as
    1. Winston X. Yan & Reza Mirzazadeh & Silvano Garnerone & David Scott & Martin W. Schneider & Tomasz Kallas & Joaquin Custodio & Erik Wernersson & Yinqing Li & Linyi Gao & Yana Federova & Bernd Zetsche &, 2017. "BLISS is a versatile and quantitative method for genome-wide profiling of DNA double-strand breaks," Nature Communications, Nature, vol. 8(1), pages 1-9, August.
    2. Nicholas S. McCarty & Alicia E. Graham & Lucie Studená & Rodrigo Ledesma-Amaro, 2020. "Multiplexed CRISPR technologies for gene editing and transcriptional regulation," Nature Communications, Nature, vol. 11(1), pages 1-13, December.
    3. Rongjie Fu & Wei He & Jinzhuang Dou & Oscar D. Villarreal & Ella Bedford & Helen Wang & Connie Hou & Liang Zhang & Yalong Wang & Dacheng Ma & Yiwen Chen & Xue Gao & Martin Depken & Han Xu, 2022. "Systematic decomposition of sequence determinants governing CRISPR/Cas9 specificity," Nature Communications, Nature, vol. 13(1), pages 1-15, December.
    4. Zhenxing Yu & Zhike Lu & Jingjing Li & Yingying Wang & Panfeng Wu & Yini Li & Yangfan Zhou & Bailun Li & Heng Zhang & Yingzheng Liu & Lijia Ma, 2022. "PEAC-seq adopts Prime Editor to detect CRISPR off-target and DNA translocation," Nature Communications, Nature, vol. 13(1), pages 1-13, December.
    5. Xiaoguang Pan & Kunli Qu & Hao Yuan & Xi Xiang & Christian Anthon & Liubov Pashkova & Xue Liang & Peng Han & Giulia I. Corsi & Fengping Xu & Ping Liu & Jiayan Zhong & Yan Zhou & Tao Ma & Hui Jiang & J, 2022. "Massively targeted evaluation of therapeutic CRISPR off-targets in cells," Nature Communications, Nature, vol. 13(1), pages 1-14, December.
    6. Jianli Tao & Qi Wang & Carlos Mendez-Dorantes & Kathleen H. Burns & Roberto Chiarle, 2022. "Frequency and mechanisms of LINE-1 retrotransposon insertions at CRISPR/Cas9 sites," Nature Communications, Nature, vol. 13(1), pages 1-17, December.
    7. Johnny H. Hu & Shannon M. Miller & Maarten H. Geurts & Weixin Tang & Liwei Chen & Ning Sun & Christina M. Zeina & Xue Gao & Holly A. Rees & Zhi Lin & David R. Liu, 2018. "Evolved Cas9 variants with broad PAM compatibility and high DNA specificity," Nature, Nature, vol. 556(7699), pages 57-63, April.
    8. Jianhang Yin & Rusen Lu & Changchang Xin & Yuhong Wang & Xinyu Ling & Dong Li & Weiwei Zhang & Mengzhu Liu & Wutao Xie & Lingyun Kong & Wen Si & Ping Wei & Bingbing Xiao & Hsiang-Ying Lee & Tao Liu & , 2022. "Cas9 exo-endonuclease eliminates chromosomal translocations during genome editing," Nature Communications, Nature, vol. 13(1), pages 1-14, December.
    9. Alexandra East-Seletsky & Mitchell R. O’Connell & Spencer C. Knight & David Burstein & Jamie H. D. Cate & Robert Tjian & Jennifer A. Doudna, 2016. "Two distinct RNase activities of CRISPR-C2c2 enable guide-RNA processing and RNA detection," Nature, Nature, vol. 538(7624), pages 270-273, October.
    10. Jungjoon K. Lee & Euihwan Jeong & Joonsun Lee & Minhee Jung & Eunji Shin & Young-hoon Kim & Kangin Lee & Inyoung Jung & Daesik Kim & Seokjoong Kim & Jin-Soo Kim, 2018. "Directed evolution of CRISPR-Cas9 to increase its specificity," Nature Communications, Nature, vol. 9(1), pages 1-10, December.
    11. Omar O. Abudayyeh & Jonathan S. Gootenberg & Patrick Essletzbichler & Shuo Han & Julia Joung & Joseph J. Belanto & Vanessa Verdine & David B. T. Cox & Max J. Kellner & Aviv Regev & Eric S. Lander & Da, 2017. "RNA targeting with CRISPR–Cas13," Nature, Nature, vol. 550(7675), pages 280-284, October.
    12. I. F. Schene & I. P. Joore & J. H. L. Baijens & R. Stevelink & G. Kok & S. Shehata & E. F. Ilcken & E. C. M. Nieuwenhuis & D. P. Bolhuis & R. C. M. Rees & S. A. Spelier & H. P. J. Doef & J. M. Beekman, 2022. "Mutation-specific reporter for optimization and enrichment of prime editing," Nature Communications, Nature, vol. 13(1), pages 1-10, December.
    13. Jeonghun Kwon & Minyoung Kim & Seungmin Bae & Anna Jo & Youngho Kim & Jungjoon K. Lee, 2022. "TAPE-seq is a cell-based method for predicting genome-wide off-target effects of prime editor," Nature Communications, Nature, vol. 13(1), pages 1-13, December.
    14. Xi Xiang & Giulia I. Corsi & Christian Anthon & Kunli Qu & Xiaoguang Pan & Xue Liang & Peng Han & Zhanying Dong & Lijun Liu & Jiayan Zhong & Tao Ma & Jinbao Wang & Xiuqing Zhang & Hui Jiang & Fengping, 2021. "Enhancing CRISPR-Cas9 gRNA efficiency prediction by data integration and deep learning," Nature Communications, Nature, vol. 12(1), pages 1-9, December.
    15. John C. Rose & Nicholas A. Popp & Christopher D. Richardson & Jason J. Stephany & Julie Mathieu & Cindy T. Wei & Jacob E. Corn & Dustin J. Maly & Douglas M. Fowler, 2020. "Suppression of unwanted CRISPR-Cas9 editing by co-administration of catalytically inactivating truncated guide RNAs," Nature Communications, Nature, vol. 11(1), pages 1-11, December.
    16. Ang Li & Hitoshi Mitsunobu & Shin Yoshioka & Takahisa Suzuki & Akihiko Kondo & Keiji Nishida, 2022. "Cytosine base editing systems with minimized off-target effect and molecular size," Nature Communications, Nature, vol. 13(1), pages 1-8, December.
    17. Fatwa Adikusuma & Sandra Piltz & Mark A. Corbett & Michelle Turvey & Shaun R. McColl & Karla J. Helbig & Michael R. Beard & James Hughes & Richard T. Pomerantz & Paul Q. Thomas, 2018. "Large deletions induced by Cas9 cleavage," Nature, Nature, vol. 560(7717), pages 8-9, August.
    18. Myungjae Song & Jung Min Lim & Seonwoo Min & Jeong-Seok Oh & Dong Young Kim & Jae-Sung Woo & Hiroshi Nishimasu & Sung-Rae Cho & Sungroh Yoon & Hyongbum Henry Kim, 2021. "Generation of a more efficient prime editor 2 by addition of the Rad51 DNA-binding domain," Nature Communications, Nature, vol. 12(1), pages 1-8, December.
    19. Jianhang Yin & Kailun Fang & Yanxia Gao & Liqiong Ou & Shaopeng Yuan & Changchang Xin & Weiwei Wu & Wei-wei Wu & Jiaxu Hong & Hui Yang & Jiazhi Hu, 2022. "Safeguarding genome integrity during gene-editing therapy in a mouse model of age-related macular degeneration," Nature Communications, Nature, vol. 13(1), pages 1-8, December.
    20. Pengpeng Liu & Shun-Qing Liang & Chunwei Zheng & Esther Mintzer & Yan G. Zhao & Karthikeyan Ponnienselvan & Aamir Mir & Erik J. Sontheimer & Guangping Gao & Terence R. Flotte & Scot A. Wolfe & Wen Xue, 2021. "Improved prime editors enable pathogenic allele correction and cancer modelling in adult mice," Nature Communications, Nature, vol. 12(1), pages 1-13, December.
    21. Grégoire Cullot & Julian Boutin & Jérôme Toutain & Florence Prat & Perrine Pennamen & Caroline Rooryck & Martin Teichmann & Emilie Rousseau & Isabelle Lamrissi-Garcia & Véronique Guyonnet-Duperat & Al, 2019. "CRISPR-Cas9 genome editing induces megabase-scale chromosomal truncations," Nature Communications, Nature, vol. 10(1), pages 1-14, December.
    22. Jianan Li & Wenxia Yu & Shisheng Huang & Susu Wu & Liping Li & Jiankui Zhou & Yu Cao & Xingxu Huang & Yunbo Qiao, 2021. "Structure-guided engineering of adenine base editor with minimized RNA off-targeting activity," Nature Communications, Nature, vol. 12(1), pages 1-8, December.
    23. Martin Pacesa & Luuk Loeff & Irma Querques & Lena M. Muckenfuss & Marta Sawicka & Martin Jinek, 2022. "R-loop formation and conformational activation mechanisms of Cas9," Nature, Nature, vol. 609(7925), pages 191-196, September.
    24. Shun-Qing Liang & Pengpeng Liu & Jordan L. Smith & Esther Mintzer & Stacy Maitland & Xiaolong Dong & Qiyuan Yang & Jonathan Lee & Cole M. Haynes & Lihua Julie Zhu & Jonathan K. Watts & Erik J. Sonthei, 2022. "Genome-wide detection of CRISPR editing in vivo using GUIDE-tag," Nature Communications, Nature, vol. 13(1), pages 1-14, December.
    25. Michael Kosicki & Felicity Allen & Frances Steward & Kärt Tomberg & Yangyang Pan & Allan Bradley, 2022. "Cas9-induced large deletions and small indels are controlled in a convergent fashion," Nature Communications, Nature, vol. 13(1), pages 1-11, December.
    26. Zhixin Lei & Haowei Meng & Lulu Liu & Huanan Zhao & Xichen Rao & Yongchang Yan & Hao Wu & Min Liu & Aibin He & Chengqi Yi, 2022. "Mitochondrial base editor induces substantial nuclear off-target mutations," Nature, Nature, vol. 606(7915), pages 804-811, June.
    27. Sébastien Levesque & Diana Mayorga & Jean-Philippe Fiset & Claudia Goupil & Alexis Duringer & Andréanne Loiselle & Eva Bouchard & Daniel Agudelo & Yannick Doyon, 2022. "Marker-free co-selection for successive rounds of prime editing in human cells," Nature Communications, Nature, vol. 13(1), pages 1-14, December.
    28. Liwei Chen & Jung Eun Park & Peter Paa & Priscilla D. Rajakumar & Hong-Ting Prekop & Yi Ting Chew & Swathi N. Manivannan & Wei Leong Chew, 2021. "Programmable C:G to G:C genome editing with CRISPR-Cas9-directed base excision repair proteins," Nature Communications, Nature, vol. 12(1), pages 1-7, December.
    29. Yajing Liu & Changyang Zhou & Shisheng Huang & Lu Dang & Yu Wei & Jun He & Yingsi Zhou & Shaoshuai Mao & Wanyu Tao & Yu Zhang & Hui Yang & Xingxu Huang & Tian Chi, 2020. "A Cas-embedding strategy for minimizing off-target effects of DNA base editors," Nature Communications, Nature, vol. 11(1), pages 1-9, December.
    30. Francesca Tucci & Stefania Galimberti & Luigi Naldini & Maria Grazia Valsecchi & Alessandro Aiuti, 2022. "A systematic review and meta-analysis of gene therapy with hematopoietic stem and progenitor cells for monogenic disorders," Nature Communications, Nature, vol. 13(1), pages 1-13, December.
    31. Beverly Y. Mok & Marcos H. de Moraes & Jun Zeng & Dustin E. Bosch & Anna V. Kotrys & Aditya Raguram & FoSheng Hsu & Matthew C. Radey & S. Brook Peterson & Vamsi K. Mootha & Joseph D. Mougous & David R, 2020. "A bacterial cytidine deaminase toxin enables CRISPR-free mitochondrial base editing," Nature, Nature, vol. 583(7817), pages 631-637, July.
    32. Xiaosa Li & Lina Zhou & Bao-Qing Gao & Guangye Li & Xiao Wang & Ying Wang & Jia Wei & Wenyan Han & Zixian Wang & Jifang Li & Runze Gao & Junjie Zhu & Wenchao Xu & Jing Wu & Bei Yang & Xiaodong Sun & L, 2022. "Highly efficient prime editing by introducing same-sense mutations in pegRNA or stabilizing its structure," Nature Communications, Nature, vol. 13(1), pages 1-9, December.
    33. Guiquan Zhang & Yao Liu & Shisheng Huang & Shiyuan Qu & Daolin Cheng & Yuan Yao & Quanjiang Ji & Xiaolong Wang & Xingxu Huang & Jianghuai Liu, 2022. "Enhancement of prime editing via xrRNA motif-joined pegRNA," Nature Communications, Nature, vol. 13(1), pages 1-12, December.
    34. Daphne Collias & Chase L. Beisel, 2021. "CRISPR technologies and the search for the PAM-free nuclease," Nature Communications, Nature, vol. 12(1), pages 1-12, December.
    35. Tanglong Yuan & Nana Yan & Tianyi Fei & Jitan Zheng & Juan Meng & Nana Li & Jing Liu & Haihang Zhang & Long Xie & Wenqin Ying & Di Li & Lei Shi & Yongsen Sun & Yongyao Li & Yixue Li & Yidi Sun & Erwei, 2021. "Optimization of C-to-G base editors with sequence context preference predictable by machine learning methods," Nature Communications, Nature, vol. 12(1), pages 1-11, December.
    36. Christopher R. Cromwell & Keewon Sung & Jinho Park & Amanda R. Krysler & Juan Jovel & Seong Keun Kim & Basil P. Hubbard, 2018. "Incorporation of bridged nucleic acids into CRISPR RNAs improves Cas9 endonuclease specificity," Nature Communications, Nature, vol. 9(1), pages 1-11, December.
    37. Felix M. Dobbs & Patrick Eijk & Mick D. Fellows & Luisa Loiacono & Roberto Nitsch & Simon H. Reed, 2022. "Precision digital mapping of endogenous and induced genomic DNA breaks by INDUCE-seq," Nature Communications, Nature, vol. 13(1), pages 1-11, December.
    38. Stamatis Papathanasiou & Styliani Markoulaki & Logan J. Blaine & Mitchell L. Leibowitz & Cheng-Zhong Zhang & Rudolf Jaenisch & David Pellman, 2021. "Whole chromosome loss and genomic instability in mouse embryos after CRISPR-Cas9 genome editing," Nature Communications, Nature, vol. 12(1), pages 1-7, December.
    39. Sanne E. Klompe & Phuc L. H. Vo & Tyler S. Halpin-Healy & Samuel H. Sternberg, 2019. "Transposon-encoded CRISPR–Cas systems direct RNA-guided DNA integration," Nature, Nature, vol. 571(7764), pages 219-225, July.
    40. Matthew A. Coelho & Etienne Braekeleer & Mike Firth & Michal Bista & Sebastian Lukasiak & Maria Emanuela Cuomo & Benjamin J. M. Taylor, 2020. "CRISPR GUARD protects off-target sites from Cas9 nuclease activity using short guide RNAs," Nature Communications, Nature, vol. 11(1), pages 1-12, December.
    41. Changchang Xin & Jianhang Yin & Shaopeng Yuan & Liqiong Ou & Mengzhu Liu & Weiwei Zhang & Jiazhi Hu, 2022. "Comprehensive assessment of miniature CRISPR-Cas12f nucleases for gene disruption," Nature Communications, Nature, vol. 13(1), pages 1-10, December.
    42. Alexis C. Komor & Yongjoo B. Kim & Michael S. Packer & John A. Zuris & David R. Liu, 2016. "Programmable editing of a target base in genomic DNA without double-stranded DNA cleavage," Nature, Nature, vol. 533(7603), pages 420-424, May.
    43. Jennifer A. Doudna, 2020. "The promise and challenge of therapeutic genome editing," Nature, Nature, vol. 578(7794), pages 229-236, February.
    44. Killian S. Hanlon & Benjamin P. Kleinstiver & Sara P. Garcia & Mikołaj P. Zaborowski & Adrienn Volak & Stefan E. Spirig & Alissa Muller & Alexander A. Sousa & Shengdar Q. Tsai & Niclas E. Bengtsson & , 2019. "High levels of AAV vector integration into CRISPR-induced DNA breaks," Nature Communications, Nature, vol. 10(1), pages 1-11, December.
    45. Péter István Kulcsár & András Tálas & Zoltán Ligeti & Sarah Laura Krausz & Ervin Welker, 2022. "SuperFi-Cas9 exhibits remarkable fidelity but severely reduced activity yet works effectively with ABE8e," Nature Communications, Nature, vol. 13(1), pages 1-11, December.
    46. Janice S. Chen & Yavuz S. Dagdas & Benjamin P. Kleinstiver & Moira M. Welch & Alexander A. Sousa & Lucas B. Harrington & Samuel H. Sternberg & J. Keith Joung & Ahmet Yildiz & Jennifer A. Doudna, 2017. "Enhanced proofreading governs CRISPR–Cas9 targeting accuracy," Nature, Nature, vol. 550(7676), pages 407-410, October.
    47. Minja Velimirovic & Larissa C. Zanetti & Max W. Shen & James D. Fife & Lin Lin & Minsun Cha & Ersin Akinci & Danielle Barnum & Tian Yu & Richard I. Sherwood, 2022. "Peptide fusion improves prime editing efficiency," Nature Communications, Nature, vol. 13(1), pages 1-8, December.
    48. Changyang Zhou & Yidi Sun & Rui Yan & Yajing Liu & Erwei Zuo & Chan Gu & Linxiao Han & Yu Wei & Xinde Hu & Rong Zeng & Yixue Li & Haibo Zhou & Fan Guo & Hui Yang, 2019. "Off-target RNA mutation induced by DNA base editing and its elimination by mutagenesis," Nature, Nature, vol. 571(7764), pages 275-278, July.
    49. Jared Carlson-Stevermer & Reed Kelso & Anastasia Kadina & Sahil Joshi & Nicholas Rossi & John Walker & Rich Stoner & Travis Maures, 2020. "CRISPRoff enables spatio-temporal control of CRISPR editing," Nature Communications, Nature, vol. 11(1), pages 1-7, December.
    50. Mu Li & Aaron Zhong & Youjun Wu & Mega Sidharta & Michael Beaury & Xiaolan Zhao & Lorenz Studer & Ting Zhou, 2022. "Transient inhibition of p53 enhances prime editing and cytosine base-editing efficiencies in human pluripotent stem cells," Nature Communications, Nature, vol. 13(1), pages 1-12, December.
    51. Tian-Lin Cheng & Shuo Li & Bo Yuan & Xiaolin Wang & Wenhao Zhou & Zilong Qiu, 2019. "Expanding C–T base editing toolkit with diversified cytidine deaminases," Nature Communications, Nature, vol. 10(1), pages 1-10, December.
    52. Martin Peterka & Nina Akrap & Songyuan Li & Sandra Wimberger & Pei-Pei Hsieh & Dmitrii Degtev & Burcu Bestas & Jack Barr & Stijn Plassche & Patricia Mendoza-Garcia & Saša Šviković & Grzegorz Sienski &, 2022. "Harnessing DSB repair to promote efficient homology-dependent and -independent prime editing," Nature Communications, Nature, vol. 13(1), pages 1-9, December.
    53. J. Boutin & J. Rosier & D. Cappellen & F. Prat & J. Toutain & P. Pennamen & J. Bouron & C. Rooryck & J. P. Merlio & I. Lamrissi-Garcia & G. Cullot & S. Amintas & V. Guyonnet-Duperat & C. Ged & J. M. B, 2021. "CRISPR-Cas9 globin editing can induce megabase-scale copy-neutral losses of heterozygosity in hematopoietic cells," Nature Communications, Nature, vol. 12(1), pages 1-12, December.
    54. Linyuan Ma & Jinxue Ruan & Jun Song & Luan Wen & Dongshan Yang & Jiangyang Zhao & Xiaofeng Xia & Y. Eugene Chen & Jifeng Zhang & Jie Xu, 2020. "MiCas9 increases large size gene knock-in rates and reduces undesirable on-target and off-target indel edits," Nature Communications, Nature, vol. 11(1), pages 1-11, December.
    55. Wei Wu & Sarah E. Hill & William J. Nathan & Jacob Paiano & Elsa Callen & Dongpeng Wang & Kenta Shinoda & Niek Wietmarschen & Jennifer M. Colón-Mercado & Dali Zong & Raffaella Pace & Han-Yu Shih & Ste, 2021. "Neuronal enhancers are hotspots for DNA single-strand break repair," Nature, Nature, vol. 593(7859), pages 440-444, May.
    56. Imre F. Schene & Indi P. Joore & Rurika Oka & Michal Mokry & Anke H. M. Vugt & Ruben Boxtel & Hubert P. J. Doef & Luc J. W. Laan & Monique M. A. Verstegen & Peter M. Hasselt & Edward E. S. Nieuwenhuis, 2020. "Prime editing for functional repair in patient-derived disease models," Nature Communications, Nature, vol. 11(1), pages 1-8, December.
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    20. Daphne Collias & Elena Vialetto & Jiaqi Yu & Khoa Co & Éva d. H. Almási & Ann-Sophie Rüttiger & Tatjana Achmedov & Till Strowig & Chase L. Beisel, 2023. "Systematically attenuating DNA targeting enables CRISPR-driven editing in bacteria," Nature Communications, Nature, vol. 14(1), pages 1-12, December.

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