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Two distinct RNase activities of CRISPR-C2c2 enable guide-RNA processing and RNA detection

Author

Listed:
  • Alexandra East-Seletsky

    (University of California)

  • Mitchell R. O’Connell

    (University of California)

  • Spencer C. Knight

    (University of California)

  • David Burstein

    (University of California)

  • Jamie H. D. Cate

    (University of California
    University of California
    Lawrence Berkeley National Laboratory)

  • Robert Tjian

    (University of California
    Janelia Research Campus, Howard Hughes Medical Institute
    Howard Hughes Medical Institute, University of California
    Li Ka Shing Biomedical and Health Sciences Center, University of California)

  • Jennifer A. Doudna

    (University of California
    University of California
    Lawrence Berkeley National Laboratory
    Howard Hughes Medical Institute, University of California)

Abstract

The CRISPR-associated bacterial enzyme C2c2 is shown to contain two separable, distinct sites for the highly sensitive detection and cleavage of single-stranded RNA.

Suggested Citation

  • Alexandra East-Seletsky & Mitchell R. O’Connell & Spencer C. Knight & David Burstein & Jamie H. D. Cate & Robert Tjian & Jennifer A. Doudna, 2016. "Two distinct RNase activities of CRISPR-C2c2 enable guide-RNA processing and RNA detection," Nature, Nature, vol. 538(7624), pages 270-273, October.
    • Handle: RePEc:nat:nature:v:538:y:2016:i:7624:d:10.1038_nature19802
    DOI: 10.1038/nature19802
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    Cited by:

    1. Anna Nemudraia & Artem Nemudryi & Murat Buyukyoruk & Andrew M. Scherffius & Trevor Zahl & Tanner Wiegand & Shishir Pandey & Joseph E. Nichols & Laina N. Hall & Aidan McVey & Helen H. Lee & Royce A. Wi, 2022. "Sequence-specific capture and concentration of viral RNA by type III CRISPR system enhances diagnostic," Nature Communications, Nature, vol. 13(1), pages 1-12, December.
    2. Shunsuke Kawasaki & Hiroki Ono & Moe Hirosawa & Takeru Kuwabara & Shunsuke Sumi & Suji Lee & Knut Woltjen & Hirohide Saito, 2023. "Programmable mammalian translational modulators by CRISPR-associated proteins," Nature Communications, Nature, vol. 14(1), pages 1-17, December.
    3. Hung-Che Kuo & Joshua Prupes & Chia-Wei Chou & Ilya J. Finkelstein, 2024. "Massively parallel profiling of RNA-targeting CRISPR-Cas13d," Nature Communications, Nature, vol. 15(1), pages 1-13, December.
    4. M. Alejandra Zeballos C. & Hayden J. Moore & Tyler J. Smith & Jackson E. Powell & Najah S. Ahsan & Sijia Zhang & Thomas Gaj, 2023. "Mitigating a TDP-43 proteinopathy by targeting ataxin-2 using RNA-targeting CRISPR effector proteins," Nature Communications, Nature, vol. 14(1), pages 1-17, December.
    5. Hongrui Zhao & Yan Sheng & Tenghua Zhang & Shujun Zhou & Yuqing Zhu & Feiyang Qian & Meiru Liu & Weixue Xu & Dengsong Zhang & Jiaming Hu, 2024. "The CRISPR-Cas13a Gemini System for noncontiguous target RNA activation," Nature Communications, Nature, vol. 15(1), pages 1-11, December.
    6. Feiyu Zhao & Tao Zhang & Xiaodi Sun & Xiyun Zhang & Letong Chen & Hejun Wang & Jinze Li & Peng Fan & Liangxue Lai & Tingting Sui & Zhanjun Li, 2023. "A strategy for Cas13 miniaturization based on the structure and AlphaFold," Nature Communications, Nature, vol. 14(1), pages 1-13, December.
    7. Xiangyu Deng & Emmanuel Osikpa & Jie Yang & Seye J. Oladeji & Jamie Smith & Xue Gao & Yang Gao, 2023. "Structural basis for the activation of a compact CRISPR-Cas13 nuclease," Nature Communications, Nature, vol. 14(1), pages 1-12, December.
    8. Jianli Tao & Daniel E. Bauer & Roberto Chiarle, 2023. "Assessing and advancing the safety of CRISPR-Cas tools: from DNA to RNA editing," Nature Communications, Nature, vol. 14(1), pages 1-16, December.
    9. Antonios Apostolopoulos & Naohiro Kawamoto & Siu Yu A. Chow & Hitomi Tsuiji & Yoshiho Ikeuchi & Yuichi Shichino & Shintaro Iwasaki, 2024. "dCas13-mediated translational repression for accurate gene silencing in mammalian cells," Nature Communications, Nature, vol. 15(1), pages 1-18, December.
    10. Yuqian Guo & Yaofeng Zhou & Hong Duan & Derong Xu & Min Wei & Yuhao Wu & Ying Xiong & Xirui Chen & Siyuan Wang & Daofeng Liu & Xiaolin Huang & Hongbo Xin & Yonghua Xiong & Ben Zhong Tang, 2024. "CRISPR/Cas-mediated “one to more” lighting-up nucleic acid detection using aggregation-induced emission luminogens," Nature Communications, Nature, vol. 15(1), pages 1-16, December.
    11. Yunxiang Wang & Hong Chen & Kai Lin & Yongjun Han & Zhixia Gu & Hongjuan Wei & Kai Mu & Dongfeng Wang & Liyan Liu & Ronghua Jin & Rui Song & Zhen Rong & Shengqi Wang, 2024. "Ultrasensitive single-step CRISPR detection of monkeypox virus in minutes with a vest-pocket diagnostic device," Nature Communications, Nature, vol. 15(1), pages 1-11, December.
    12. Ning Cui & Jun-Tao Zhang & Zhuolin Li & Xiao-Yu Liu & Chongyuan Wang & Hongda Huang & Ning Jia, 2022. "Structural basis for the non-self RNA-activated protease activity of the type III-E CRISPR nuclease-protease Craspase," Nature Communications, Nature, vol. 13(1), pages 1-13, December.

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