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CRISPRoff enables spatio-temporal control of CRISPR editing

Author

Listed:
  • Jared Carlson-Stevermer

    (Synthego Corporation)

  • Reed Kelso

    (Synthego Corporation
    Freenome)

  • Anastasia Kadina

    (Synthego Corporation)

  • Sahil Joshi

    (Synthego Corporation)

  • Nicholas Rossi

    (Synthego Corporation)

  • John Walker

    (Synthego Corporation)

  • Rich Stoner

    (Synthego Corporation)

  • Travis Maures

    (Synthego Corporation)

Abstract

Following introduction of CRISPR-Cas9 components into a cell, genome editing occurs unabated until degradation of its component nucleic acids and proteins by cellular processes. This uncontrolled reaction can lead to unintended consequences including off-target editing and chromosomal translocations. To address this, we develop a method for light-induced degradation of sgRNA termed CRISPRoff. Here we show that light-induced inactivation of ribonucleoprotein attenuates genome editing within cells and allows for titratable levels of editing efficiency and spatial patterning via selective illumination.

Suggested Citation

  • Jared Carlson-Stevermer & Reed Kelso & Anastasia Kadina & Sahil Joshi & Nicholas Rossi & John Walker & Rich Stoner & Travis Maures, 2020. "CRISPRoff enables spatio-temporal control of CRISPR editing," Nature Communications, Nature, vol. 11(1), pages 1-7, December.
    • Handle: RePEc:nat:natcom:v:11:y:2020:i:1:d:10.1038_s41467-020-18853-3
    DOI: 10.1038/s41467-020-18853-3
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    Cited by:

    1. Jianli Tao & Daniel E. Bauer & Roberto Chiarle, 2023. "Assessing and advancing the safety of CRISPR-Cas tools: from DNA to RNA editing," Nature Communications, Nature, vol. 14(1), pages 1-16, December.

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