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Expanding C–T base editing toolkit with diversified cytidine deaminases

Author

Listed:
  • Tian-Lin Cheng

    (Chinese Academy of Sciences)

  • Shuo Li

    (Zhongshan Hospital, Fudan University)

  • Bo Yuan

    (Chinese Academy of Sciences)

  • Xiaolin Wang

    (Zhongshan Hospital, Fudan University
    Shanghai Institute of Medical Imaging)

  • Wenhao Zhou

    (Children’s Hospital of Fudan University)

  • Zilong Qiu

    (Chinese Academy of Sciences)

Abstract

Base editing tools for cytosine to thymine (C–T) conversion enable genome manipulation at single base-pair resolution with high efficiency. Available base editors (BEs) for C–T conversion (CBEs) have restricted editing scopes and nonnegligible off-target effects, which limit their applications. Here, by screening diversified lamprey cytidine deaminases, we establish various CBEs with expanded and diversified editing scopes, which could be further refined by various fusing strategies, fusing at either N-terminus or C–terminus of nCas9. Furthermore, off-target analysis reveals that several CBEs display improved fidelity. Our study expands the toolkits for C–T conversion, serves as guidance for appropriate choice and offers a framework for benchmarking future improvement of base editing tools.

Suggested Citation

  • Tian-Lin Cheng & Shuo Li & Bo Yuan & Xiaolin Wang & Wenhao Zhou & Zilong Qiu, 2019. "Expanding C–T base editing toolkit with diversified cytidine deaminases," Nature Communications, Nature, vol. 10(1), pages 1-10, December.
    • Handle: RePEc:nat:natcom:v:10:y:2019:i:1:d:10.1038_s41467-019-11562-6
    DOI: 10.1038/s41467-019-11562-6
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    Cited by:

    1. Jianli Tao & Daniel E. Bauer & Roberto Chiarle, 2023. "Assessing and advancing the safety of CRISPR-Cas tools: from DNA to RNA editing," Nature Communications, Nature, vol. 14(1), pages 1-16, December.

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