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Programmable editing of a target base in genomic DNA without double-stranded DNA cleavage

Author

Listed:
  • Alexis C. Komor

    (Harvard University
    Howard Hughes Medical Institute, Harvard University)

  • Yongjoo B. Kim

    (Harvard University
    Howard Hughes Medical Institute, Harvard University)

  • Michael S. Packer

    (Harvard University
    Howard Hughes Medical Institute, Harvard University)

  • John A. Zuris

    (Harvard University
    Howard Hughes Medical Institute, Harvard University)

  • David R. Liu

    (Harvard University
    Howard Hughes Medical Institute, Harvard University)

Abstract

CRISPR/Cas9 DNA editing creates a double-stranded break in the target DNA, which can frequently generate random insertion or deletion of bases (indels); a new genome editing approach combining Cas9 with a cytidine deaminase is described here, which corrects point mutations more efficiently than canonical Cas9, while avoiding double-stranded breaks and indel formation.

Suggested Citation

  • Alexis C. Komor & Yongjoo B. Kim & Michael S. Packer & John A. Zuris & David R. Liu, 2016. "Programmable editing of a target base in genomic DNA without double-stranded DNA cleavage," Nature, Nature, vol. 533(7603), pages 420-424, May.
    • Handle: RePEc:nat:nature:v:533:y:2016:i:7603:d:10.1038_nature17946
    DOI: 10.1038/nature17946
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