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RNA targeting with CRISPR–Cas13

Author

Listed:
  • Omar O. Abudayyeh

    (Broad Institute of MIT and Harvard
    McGovern Institute for Brain Research at MIT
    Massachusetts Institute of Technology
    Massachusetts Institute of Technology)

  • Jonathan S. Gootenberg

    (Broad Institute of MIT and Harvard
    McGovern Institute for Brain Research at MIT
    Massachusetts Institute of Technology
    Massachusetts Institute of Technology)

  • Patrick Essletzbichler

    (Broad Institute of MIT and Harvard
    McGovern Institute for Brain Research at MIT
    Massachusetts Institute of Technology
    Massachusetts Institute of Technology)

  • Shuo Han

    (Biology, and Chemistry, Stanford University)

  • Julia Joung

    (Broad Institute of MIT and Harvard
    McGovern Institute for Brain Research at MIT
    Massachusetts Institute of Technology
    Massachusetts Institute of Technology)

  • Joseph J. Belanto

    (Cell Biology and Development, University of Minnesota
    Center for Genome Engineering, University of Minnesota)

  • Vanessa Verdine

    (Broad Institute of MIT and Harvard
    McGovern Institute for Brain Research at MIT
    Massachusetts Institute of Technology
    Massachusetts Institute of Technology)

  • David B. T. Cox

    (Broad Institute of MIT and Harvard
    McGovern Institute for Brain Research at MIT
    Massachusetts Institute of Technology
    Massachusetts Institute of Technology)

  • Max J. Kellner

    (Broad Institute of MIT and Harvard)

  • Aviv Regev

    (Broad Institute of MIT and Harvard
    Massachusetts Institute of Technology)

  • Eric S. Lander

    (Broad Institute of MIT and Harvard
    Harvard Medical School
    Massachusetts Institute of Technology)

  • Daniel F. Voytas

    (Cell Biology and Development, University of Minnesota
    Center for Genome Engineering, University of Minnesota)

  • Alice Y. Ting

    (Biology, and Chemistry, Stanford University)

  • Feng Zhang

    (Broad Institute of MIT and Harvard
    McGovern Institute for Brain Research at MIT
    Massachusetts Institute of Technology
    Massachusetts Institute of Technology)

Abstract

The class 2 type VI RNA-guided RNA-targeting CRISPR–Cas effector Cas13 can be engineered for RNA knockdown and binding, expanding the CRISPR toolset with a flexible platform for studying RNA in mammalian cells and therapeutic development.

Suggested Citation

  • Omar O. Abudayyeh & Jonathan S. Gootenberg & Patrick Essletzbichler & Shuo Han & Julia Joung & Joseph J. Belanto & Vanessa Verdine & David B. T. Cox & Max J. Kellner & Aviv Regev & Eric S. Lander & Da, 2017. "RNA targeting with CRISPR–Cas13," Nature, Nature, vol. 550(7675), pages 280-284, October.
    • Handle: RePEc:nat:nature:v:550:y:2017:i:7675:d:10.1038_nature24049
    DOI: 10.1038/nature24049
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    Citations

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    Cited by:

    1. M. Alejandra Zeballos C. & Hayden J. Moore & Tyler J. Smith & Jackson E. Powell & Najah S. Ahsan & Sijia Zhang & Thomas Gaj, 2023. "Mitigating a TDP-43 proteinopathy by targeting ataxin-2 using RNA-targeting CRISPR effector proteins," Nature Communications, Nature, vol. 14(1), pages 1-17, December.
    2. Lauren A. Blake & Leslie Watkins & Yang Liu & Takanari Inoue & Bin Wu, 2024. "A rapid inducible RNA decay system reveals fast mRNA decay in P-bodies," Nature Communications, Nature, vol. 15(1), pages 1-14, December.
    3. Hongrui Zhao & Yan Sheng & Tenghua Zhang & Shujun Zhou & Yuqing Zhu & Feiyang Qian & Meiru Liu & Weixue Xu & Dengsong Zhang & Jiaming Hu, 2024. "The CRISPR-Cas13a Gemini System for noncontiguous target RNA activation," Nature Communications, Nature, vol. 15(1), pages 1-11, December.
    4. Feiyu Zhao & Tao Zhang & Xiaodi Sun & Xiyun Zhang & Letong Chen & Hejun Wang & Jinze Li & Peng Fan & Liangxue Lai & Tingting Sui & Zhanjun Li, 2023. "A strategy for Cas13 miniaturization based on the structure and AlphaFold," Nature Communications, Nature, vol. 14(1), pages 1-13, December.
    5. David N. Fiflis & Nicolas A. Rey & Harshitha Venugopal-Lavanya & Beatrice Sewell & Aaron Mitchell-Dick & Katie N. Clements & Sydney Milo & Abigail R. Benkert & Alan Rosales & Sophia Fergione & Aravind, 2024. "Repurposing CRISPR-Cas13 systems for robust mRNA trans-splicing," Nature Communications, Nature, vol. 15(1), pages 1-14, December.
    6. Pei Liu & Josquin Foiret & Yinglin Situ & Nisi Zhang & Aris J. Kare & Bo Wu & Marina N. Raie & Katherine W. Ferrara & Lei S. Qi, 2023. "Sonogenetic control of multiplexed genome regulation and base editing," Nature Communications, Nature, vol. 14(1), pages 1-11, December.
    7. Evan A. Schwartz & Jack P. K. Bravo & Mohd Ahsan & Luis A. Macias & Caitlyn L. McCafferty & Tyler L. Dangerfield & Jada N. Walker & Jennifer S. Brodbelt & Giulia Palermo & Peter C. Fineran & Robert D., 2024. "RNA targeting and cleavage by the type III-Dv CRISPR effector complex," Nature Communications, Nature, vol. 15(1), pages 1-14, December.
    8. Zhifang Li & Ruochen Guo & Xiaozhi Sun & Guoling Li & Zhuang Shao & Xiaona Huo & Rongrong Yang & Xinyu Liu & Xi Cao & Hainan Zhang & Weihong Zhang & Xiaoyin Zhang & Shuangyu Ma & Meiling Zhang & Yuanh, 2024. "Engineering a transposon-associated TnpB-ωRNA system for efficient gene editing and phenotypic correction of a tyrosinaemia mouse model," Nature Communications, Nature, vol. 15(1), pages 1-11, December.
    9. Xiangyu Deng & Emmanuel Osikpa & Jie Yang & Seye J. Oladeji & Jamie Smith & Xue Gao & Yang Gao, 2023. "Structural basis for the activation of a compact CRISPR-Cas13 nuclease," Nature Communications, Nature, vol. 14(1), pages 1-12, December.
    10. Breanna DiAndreth & Noreen Wauford & Eileen Hu & Sebastian Palacios & Ron Weiss, 2022. "PERSIST platform provides programmable RNA regulation using CRISPR endoRNases," Nature Communications, Nature, vol. 13(1), pages 1-11, December.
    11. Jianli Tao & Daniel E. Bauer & Roberto Chiarle, 2023. "Assessing and advancing the safety of CRISPR-Cas tools: from DNA to RNA editing," Nature Communications, Nature, vol. 14(1), pages 1-16, December.
    12. Ying Liang & Haiyue Xu & Tao Cheng & Yujuan Fu & Hanwei Huang & Wenchang Qian & Junyan Wang & Yuenan Zhou & Pengxu Qian & Yafei Yin & Pengfei Xu & Wei Zou & Baohui Chen, 2022. "Gene activation guided by nascent RNA-bound transcription factors," Nature Communications, Nature, vol. 13(1), pages 1-16, December.
    13. Huaxia Shi & Ying Xu & Na Tian & Ming Yang & Fu-Sen Liang, 2022. "Inducible and reversible RNA N6-methyladenosine editing," Nature Communications, Nature, vol. 13(1), pages 1-10, December.
    14. Yage Ding & Cristina Tous & Jaehoon Choi & Jingyao Chen & Wilson W. Wong, 2024. "Orthogonal inducible control of Cas13 circuits enables programmable RNA regulation in mammalian cells," Nature Communications, Nature, vol. 15(1), pages 1-16, December.
    15. Xiaolong Cheng & Zexu Li & Ruocheng Shan & Zihan Li & Shengnan Wang & Wenchang Zhao & Han Zhang & Lumen Chao & Jian Peng & Teng Fei & Wei Li, 2023. "Modeling CRISPR-Cas13d on-target and off-target effects using machine learning approaches," Nature Communications, Nature, vol. 14(1), pages 1-14, December.
    16. Jeonghye Yu & Jongpil Shin & Jihwan Yu & Jihye Kim & Daseuli Yu & Won Do Heo, 2024. "Programmable RNA base editing with photoactivatable CRISPR-Cas13," Nature Communications, Nature, vol. 15(1), pages 1-14, December.
    17. Yuehan Yang & Mara Grace C. Kessler & M. Raquel Marchán-Rivadeneira & Yuxi Zhou & Yong Han, 2024. "Adeno-Associated Virus-Mediated CRISPR-Cas13 Knockdown of Papain-like Protease from SARS-CoV-2 Virus," J, MDPI, vol. 7(3), pages 1-13, September.
    18. Antonios Apostolopoulos & Naohiro Kawamoto & Siu Yu A. Chow & Hitomi Tsuiji & Yoshiho Ikeuchi & Yuichi Shichino & Shintaro Iwasaki, 2024. "dCas13-mediated translational repression for accurate gene silencing in mammalian cells," Nature Communications, Nature, vol. 15(1), pages 1-18, December.
    19. Yocelyn Recinos & Dmytro Ustianenko & Yow-Tyng Yeh & Xiaojian Wang & Martin Jacko & Lekha V. Yesantharao & Qiyang Wu & Chaolin Zhang, 2024. "CRISPR-dCas13d-based deep screening of proximal and distal splicing-regulatory elements," Nature Communications, Nature, vol. 15(1), pages 1-15, December.
    20. Amanda R. Krysler & Christopher R. Cromwell & Tommy Tu & Juan Jovel & Basil P. Hubbard, 2022. "Guide RNAs containing universal bases enable Cas9/Cas12a recognition of polymorphic sequences," Nature Communications, Nature, vol. 13(1), pages 1-13, December.
    21. Duško Lainšček & Vida Forstnerič & Veronika Mikolič & Špela Malenšek & Peter Pečan & Mojca Benčina & Matjaž Sever & Helena Podgornik & Roman Jerala, 2022. "Coiled-coil heterodimer-based recruitment of an exonuclease to CRISPR/Cas for enhanced gene editing," Nature Communications, Nature, vol. 13(1), pages 1-12, December.
    22. Mingyu Yao & Zhenhai Zeng & Siheng Li & Zhilin Zou & Zhongxing Chen & Xinyi Chen & Qingyi Gao & Guoli Zhao & Aodong Chen & Zheng Li & Yiran Wang & Rui Ning & Colm McAlinden & Xingtao Zhou & Jinhai Hua, 2024. "CRISPR-CasRx-mediated disruption of Aqp1/Adrb2/Rock1/Rock2 genes reduces intraocular pressure and retinal ganglion cell damage in mice," Nature Communications, Nature, vol. 15(1), pages 1-15, December.

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