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Generation of a more efficient prime editor 2 by addition of the Rad51 DNA-binding domain

Author

Listed:
  • Myungjae Song

    (Yonsei University College of Medicine
    Yonsei University College of Medicine)

  • Jung Min Lim

    (Yonsei University College of Medicine)

  • Seonwoo Min

    (Seoul National University)

  • Jeong-Seok Oh

    (Korea University)

  • Dong Young Kim

    (Yonsei University College of Medicine
    Yonsei University College of Medicine)

  • Jae-Sung Woo

    (Korea University)

  • Hiroshi Nishimasu

    (The University of Tokyo)

  • Sung-Rae Cho

    (Yonsei University College of Medicine
    Yonsei University College of Medicine
    Yonsei University
    Yonsei University College of Medicine)

  • Sungroh Yoon

    (Seoul National University
    Seoul National University)

  • Hyongbum Henry Kim

    (Yonsei University College of Medicine
    Yonsei University College of Medicine
    Yonsei University
    Institute for Basic Science (IBS))

Abstract

Although prime editing is a promising genome editing method, the efficiency of prime editor 2 (PE2) is often insufficient. Here we generate a more efficient variant of PE2, named hyPE2, by adding the Rad51 DNA-binding domain. When tested at endogenous sites, hyPE2 shows a median of 1.5- or 1.4- fold (range, 0.99- to 2.6-fold) higher efficiencies than PE2; furthermore, at sites where PE2-induced prime editing is very inefficient (efficiency

Suggested Citation

  • Myungjae Song & Jung Min Lim & Seonwoo Min & Jeong-Seok Oh & Dong Young Kim & Jae-Sung Woo & Hiroshi Nishimasu & Sung-Rae Cho & Sungroh Yoon & Hyongbum Henry Kim, 2021. "Generation of a more efficient prime editor 2 by addition of the Rad51 DNA-binding domain," Nature Communications, Nature, vol. 12(1), pages 1-8, December.
    • Handle: RePEc:nat:natcom:v:12:y:2021:i:1:d:10.1038_s41467-021-25928-2
    DOI: 10.1038/s41467-021-25928-2
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    Cited by:

    1. Chunwei Zheng & Bin Liu & Xiaolong Dong & Nicholas Gaston & Erik J. Sontheimer & Wen Xue, 2023. "Template-jumping prime editing enables large insertion and exon rewriting in vivo," Nature Communications, Nature, vol. 14(1), pages 1-9, December.
    2. Jianli Tao & Daniel E. Bauer & Roberto Chiarle, 2023. "Assessing and advancing the safety of CRISPR-Cas tools: from DNA to RNA editing," Nature Communications, Nature, vol. 14(1), pages 1-16, December.
    3. Ronghao Chen & Yu Cao & Yajing Liu & Dongdong Zhao & Ju Li & Zhihui Cheng & Changhao Bi & Xueli Zhang, 2023. "Enhancement of a prime editing system via optimal recruitment of the pioneer transcription factor P65," Nature Communications, Nature, vol. 14(1), pages 1-8, December.
    4. Jeonghun Kwon & Minyoung Kim & Seungmin Bae & Anna Jo & Youngho Kim & Jungjoon K. Lee, 2022. "TAPE-seq is a cell-based method for predicting genome-wide off-target effects of prime editor," Nature Communications, Nature, vol. 13(1), pages 1-13, December.
    5. Xiangyang Li & Guiquan Zhang & Shisheng Huang & Yao Liu & Jin Tang & Mingtian Zhong & Xin Wang & Wenjun Sun & Yuan Yao & Quanjiang Ji & Xiaolong Wang & Jianghuai Liu & Shiqiang Zhu & Xingxu Huang, 2023. "Development of a versatile nuclease prime editor with upgraded precision," Nature Communications, Nature, vol. 14(1), pages 1-13, December.

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