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A membrane associated tandem kinase from wild emmer wheat confers broad-spectrum resistance to powdery mildew

Author

Listed:
  • Miaomiao Li

    (Chinese Academy of Sciences)

  • Huaizhi Zhang

    (Chinese Academy of Sciences)

  • Huixin Xiao

    (Chinese Academy of Sciences)

  • Keyu Zhu

    (Chinese Academy of Sciences
    University of Chinese Academy of Sciences)

  • Wenqi Shi

    (Hubei Academy of Agricultural Sciences)

  • Dong Zhang

    (Beijing PlantTech Biotechnology Co., Ltd.)

  • Yong Wang

    (Chinese Academy of Agricultural Sciences)

  • Lijun Yang

    (Hubei Academy of Agricultural Sciences)

  • Qiuhong Wu

    (Chinese Academy of Sciences)

  • Jingzhong Xie

    (Chinese Academy of Sciences)

  • Yongxing Chen

    (Chinese Academy of Sciences
    University of Chinese Academy of Sciences)

  • Dan Qiu

    (Chinese Academy of Sciences)

  • Guanghao Guo

    (Chinese Academy of Sciences)

  • Ping Lu

    (Chinese Academy of Sciences)

  • Beibei Li

    (Chinese Academy of Sciences
    University of Chinese Academy of Sciences)

  • Lei Dong

    (Chinese Academy of Sciences
    University of Chinese Academy of Sciences)

  • Wenling Li

    (Chinese Academy of Sciences
    University of Chinese Academy of Sciences)

  • Xuejia Cui

    (Chinese Academy of Sciences
    University of Chinese Academy of Sciences)

  • Lingchuan Li

    (Chinese Academy of Sciences
    University of Chinese Academy of Sciences)

  • Xiubin Tian

    (Chinese Academy of Sciences)

  • Chengguo Yuan

    (Hebei Gaoyi Seeds Farm)

  • Yiwen Li

    (Chinese Academy of Sciences)

  • Dazhao Yu

    (Hubei Academy of Agricultural Sciences)

  • Eviatar Nevo

    (University of Haifa)

  • Tzion Fahima

    (University of Haifa)

  • Hongjie Li

    (Chinese Academy of Agricultural Sciences)

  • Lingli Dong

    (Chinese Academy of Sciences)

  • Yusheng Zhao

    (Chinese Academy of Sciences
    University of Chinese Academy of Sciences)

  • Zhiyong Liu

    (Chinese Academy of Sciences
    University of Chinese Academy of Sciences
    Hainan Seed Industry Laboratory)

Abstract

Crop wild relatives offer natural variations of disease resistance for crop improvement. Here, we report the isolation of broad-spectrum powdery mildew resistance gene Pm36, originated from wild emmer wheat, that encodes a tandem kinase with a transmembrane domain (WTK7-TM) through the combination of map-based cloning, PacBio SMRT long-read genome sequencing, mutagenesis, and transformation. Mutagenesis assay reveals that the two kinase domains and the transmembrane domain of WTK7-TM are critical for the powdery mildew resistance function. Consistently, in vitro phosphorylation assay shows that two kinase domains are indispensable for the kinase activity of WTK7-TM. Haplotype analysis uncovers that Pm36 is an orphan gene only present in a few wild emmer wheat, indicating its single ancient origin and potential contribution to the current wheat gene pool. Overall, our findings not only provide a powdery mildew resistance gene with great potential in wheat breeding but also sheds light into the mechanism underlying broad-spectrum resistance.

Suggested Citation

  • Miaomiao Li & Huaizhi Zhang & Huixin Xiao & Keyu Zhu & Wenqi Shi & Dong Zhang & Yong Wang & Lijun Yang & Qiuhong Wu & Jingzhong Xie & Yongxing Chen & Dan Qiu & Guanghao Guo & Ping Lu & Beibei Li & Lei, 2024. "A membrane associated tandem kinase from wild emmer wheat confers broad-spectrum resistance to powdery mildew," Nature Communications, Nature, vol. 15(1), pages 1-14, December.
    • Handle: RePEc:nat:natcom:v:15:y:2024:i:1:d:10.1038_s41467-024-47497-w
    DOI: 10.1038/s41467-024-47497-w
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    2. Chao Ma & Xiubin Tian & Zhenjie Dong & Huanhuan Li & Xuexue Chen & Wenxuan Liu & Guihong Yin & Shuyang Ma & Liwei Zhang & Aizhong Cao & Cheng Liu & Hongfei Yan & Sunish K. Sehgal & Zhibin Zhang & Bao , 2024. "An Aegilops longissima NLR protein with integrated CC-BED module mediates resistance to wheat powdery mildew," Nature Communications, Nature, vol. 15(1), pages 1-13, December.
    3. Huagang He & Zhaozhao Chen & Renchun Fan & Jie Zhang & Shanying Zhu & Jiale Wang & Qianyuan Zhang & Anli Gao & Shuangjun Gong & Lu Zhang & Yanan Li & Yitong Zhao & Simon G. Krattinger & Qian-Hua Shen , 2024. "A kinase fusion protein from Aegilops longissima confers resistance to wheat powdery mildew," Nature Communications, Nature, vol. 15(1), pages 1-13, December.

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