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A High Throughput Barley Stripe Mosaic Virus Vector for Virus Induced Gene Silencing in Monocots and Dicots

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  • Cheng Yuan
  • Cui Li
  • Lijie Yan
  • Andrew O Jackson
  • Zhiyong Liu
  • Chenggui Han
  • Jialin Yu
  • Dawei Li

Abstract

Barley stripe mosaic virus (BSMV) is a single-stranded RNA virus with three genome components designated alpha, beta, and gamma. BSMV vectors have previously been shown to be efficient virus induced gene silencing (VIGS) vehicles in barley and wheat and have provided important information about host genes functioning during pathogenesis as well as various aspects of genes functioning in development. To permit more effective use of BSMV VIGS for functional genomics experiments, we have developed an Agrobacterium delivery system for BSMV and have coupled this with a ligation independent cloning (LIC) strategy to mediate efficient cloning of host genes. Infiltrated Nicotiana benthamiana leaves provided excellent sources of virus for secondary BSMV infections and VIGS in cereals. The Agro/LIC BSMV VIGS vectors were able to function in high efficiency down regulation of phytoene desaturase (PDS), magnesium chelatase subunit H (ChlH), and plastid transketolase (TK) gene silencing in N. benthamiana and in the monocots, wheat, barley, and the model grass, Brachypodium distachyon. Suppression of an Arabidopsis orthologue cloned from wheat (TaPMR5) also interfered with wheat powdery mildew (Blumeria graminis f. sp. tritici) infections in a manner similar to that of the A. thaliana PMR5 loss-of-function allele. These results imply that the PMR5 gene has maintained similar functions across monocot and dicot families. Our BSMV VIGS system provides substantial advantages in expense, cloning efficiency, ease of manipulation and ability to apply VIGS for high throughput genomics studies.

Suggested Citation

  • Cheng Yuan & Cui Li & Lijie Yan & Andrew O Jackson & Zhiyong Liu & Chenggui Han & Jialin Yu & Dawei Li, 2011. "A High Throughput Barley Stripe Mosaic Virus Vector for Virus Induced Gene Silencing in Monocots and Dicots," PLOS ONE, Public Library of Science, vol. 6(10), pages 1-16, October.
    • Handle: RePEc:plo:pone00:0026468
    DOI: 10.1371/journal.pone.0026468
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    Cited by:

    1. Jian Jiao & Yichun Wang & Jonathan Nimal Selvaraj & Fuguo Xing & Yang Liu, 2015. "Barley Stripe Mosaic Virus (BSMV) Induced MicroRNA Silencing in Common Wheat (Triticum aestivum L.)," PLOS ONE, Public Library of Science, vol. 10(5), pages 1-13, May.
    2. Miaomiao Li & Huaizhi Zhang & Huixin Xiao & Keyu Zhu & Wenqi Shi & Dong Zhang & Yong Wang & Lijun Yang & Qiuhong Wu & Jingzhong Xie & Yongxing Chen & Dan Qiu & Guanghao Guo & Ping Lu & Beibei Li & Lei, 2024. "A membrane associated tandem kinase from wild emmer wheat confers broad-spectrum resistance to powdery mildew," Nature Communications, Nature, vol. 15(1), pages 1-14, December.
    3. Stuart D. Desjardins & James Simmonds & Inna Guterman & Kostya Kanyuka & Amanda J. Burridge & Andrew J. Tock & Eugenio Sanchez-Moran & F. Chris H. Franklin & Ian R. Henderson & Keith J. Edwards & Cris, 2022. "FANCM promotes class I interfering crossovers and suppresses class II non-interfering crossovers in wheat meiosis," Nature Communications, Nature, vol. 13(1), pages 1-12, December.
    4. Guifang Lin & Hui Chen & Bin Tian & Sunish K. Sehgal & Lovepreet Singh & Jingzhong Xie & Nidhi Rawat & Philomin Juliana & Narinder Singh & Sandesh Shrestha & Duane L. Wilson & Hannah Shult & Hyeonju L, 2022. "Cloning of the broadly effective wheat leaf rust resistance gene Lr42 transferred from Aegilops tauschii," Nature Communications, Nature, vol. 13(1), pages 1-12, December.

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