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An Aegilops longissima NLR protein with integrated CC-BED module mediates resistance to wheat powdery mildew

Author

Listed:
  • Chao Ma

    (Henan Agricultural University)

  • Xiubin Tian

    (Chinese Academy of Sciences
    University of Chinese Academy of Sciences)

  • Zhenjie Dong

    (Nanjing Agricultural University)

  • Huanhuan Li

    (Henan Agricultural University)

  • Xuexue Chen

    (Chinese Academy of Sciences)

  • Wenxuan Liu

    (Henan Agricultural University)

  • Guihong Yin

    (Henan Agricultural University)

  • Shuyang Ma

    (Chinese Academy of Sciences
    University of Chinese Academy of Sciences)

  • Liwei Zhang

    (China Agricultural University)

  • Aizhong Cao

    (Nanjing Agricultural University)

  • Cheng Liu

    (Shandong Academy of Agricultural Sciences)

  • Hongfei Yan

    (Hebei Agricultural University)

  • Sunish K. Sehgal

    (South Dakota State University)

  • Zhibin Zhang

    (Northeast Normal University)

  • Bao Liu

    (Northeast Normal University)

  • Shiwei Wang

    (China Agricultural University)

  • Qianwen Liu

    (Henan Agricultural University)

  • Yusheng Zhao

    (Chinese Academy of Sciences)

  • Yue Zhao

    (Henan Agricultural University)

Abstract

Powdery mildew, caused by Blumeria graminis f. sp. tritici (Bgt), reduces wheat yields and grain quality, thus posing a significant threat to global food security. Wild relatives of wheat serve as valuable resources for resistance to powdery mildew. Here, the powdery mildew resistance gene Pm6Sl is cloned from the wild wheat species Aegilops longissima. It encodes a nucleotide-binding leucine-rich repeat (NLR) protein featuring a CC-BED module formed by a zinc finger BED (Znf-BED) domain integrated into the coiled-coil (CC) domain. The function of Pm6Sl is validated via mutagenesis, gene silencing, and transgenic assays. In addition, we develop a resistant germplasm harbouring Pm6Sl in a very small segment with no linkage drag along with the diagnostic gene marker pm6sl-1 to facilitate Pm6Sl deployment in wheat breeding programs. The cloning of Pm6Sl, a resistance gene with BED-NLR architecture, will increase our understanding of the molecular mechanisms underlying BED-NLR-mediated resistance to various pathogens.

Suggested Citation

  • Chao Ma & Xiubin Tian & Zhenjie Dong & Huanhuan Li & Xuexue Chen & Wenxuan Liu & Guihong Yin & Shuyang Ma & Liwei Zhang & Aizhong Cao & Cheng Liu & Hongfei Yan & Sunish K. Sehgal & Zhibin Zhang & Bao , 2024. "An Aegilops longissima NLR protein with integrated CC-BED module mediates resistance to wheat powdery mildew," Nature Communications, Nature, vol. 15(1), pages 1-13, December.
    • Handle: RePEc:nat:natcom:v:15:y:2024:i:1:d:10.1038_s41467-024-52670-2
    DOI: 10.1038/s41467-024-52670-2
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    References listed on IDEAS

    as
    1. Huanhuan Li & Wenqiang Men & Chao Ma & Qianwen Liu & Zhenjie Dong & Xiubin Tian & Chaoli Wang & Cheng Liu & Harsimardeep S. Gill & Pengtao Ma & Zhibin Zhang & Bao Liu & Yue Zhao & Sunish K. Sehgal & W, 2024. "Wheat powdery mildew resistance gene Pm13 encodes a mixed lineage kinase domain-like protein," Nature Communications, Nature, vol. 15(1), pages 1-13, December.
    2. Miaomiao Li & Huaizhi Zhang & Huixin Xiao & Keyu Zhu & Wenqi Shi & Dong Zhang & Yong Wang & Lijun Yang & Qiuhong Wu & Jingzhong Xie & Yongxing Chen & Dan Qiu & Guanghao Guo & Ping Lu & Beibei Li & Lei, 2024. "A membrane associated tandem kinase from wild emmer wheat confers broad-spectrum resistance to powdery mildew," Nature Communications, Nature, vol. 15(1), pages 1-14, December.
    3. Chuntian Lu & Jie Du & Heyu Chen & Shuangjun Gong & Yinyu Jin & Xiangru Meng & Ting Zhang & Bisheng Fu & István Molnár & Kateřina Holušová & Mahmoud Said & Liping Xing & Lingna Kong & Jaroslav Doležel, 2024. "Wheat Pm55 alleles exhibit distinct interactions with an inhibitor to cause different powdery mildew resistance," Nature Communications, Nature, vol. 15(1), pages 1-13, December.
    4. Yue Zhao & Zhenjie Dong & Jingnan Miao & Qianwen Liu & Chao Ma & Xiubin Tian & Jinqiu He & Huihui Bi & Wen Yao & Tao Li & Harsimardeep S. Gill & Zhibin Zhang & Aizhong Cao & Bao Liu & Huanhuan Li & Su, 2024. "Pm57 from Aegilops searsii encodes a tandem kinase protein and confers wheat powdery mildew resistance," Nature Communications, Nature, vol. 15(1), pages 1-13, December.
    5. Hongna Li & Lei Hua & Shuqing Zhao & Ming Hao & Rui Song & Shuyong Pang & Yanna Liu & Hong Chen & Wenjun Zhang & Tao Shen & Jin-Ying Gou & Hailiang Mao & Guiping Wang & Xiaohua Hao & Jian Li & Baoxing, 2023. "Cloning of the wheat leaf rust resistance gene Lr47 introgressed from Aegilops speltoides," Nature Communications, Nature, vol. 14(1), pages 1-15, December.
    6. Fei Ni & Yanyan Zheng & Xiaoke Liu & Yang Yu & Guangqiang Zhang & Lynn Epstein & Xue Mao & Jingzheng Wu & Cuiling Yuan & Bo Lv & Haixia Yu & Jinlong Li & Qi Zhao & Qiyu Yang & Jiajun Liu & Juan Qi & D, 2023. "Sequencing trait-associated mutations to clone wheat rust-resistance gene YrNAM," Nature Communications, Nature, vol. 14(1), pages 1-8, December.
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