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Cloning of the broadly effective wheat leaf rust resistance gene Lr42 transferred from Aegilops tauschii

Author

Listed:
  • Guifang Lin

    (Kansas State University)

  • Hui Chen

    (Kansas State University)

  • Bin Tian

    (Kansas State University
    Research Triangle Park)

  • Sunish K. Sehgal

    (South Dakota State University)

  • Lovepreet Singh

    (University of Maryland)

  • Jingzhong Xie

    (Kansas State University
    Chinese Academy of Sciences)

  • Nidhi Rawat

    (University of Maryland)

  • Philomin Juliana

    (International Maize and Wheat Improvement Center (CIMMYT)
    Borlaug Institute for South Asia)

  • Narinder Singh

    (Kansas State University
    Bayer R&D Services LLC)

  • Sandesh Shrestha

    (Kansas State University)

  • Duane L. Wilson

    (Kansas State University)

  • Hannah Shult

    (Kansas State University)

  • Hyeonju Lee

    (Kansas State University)

  • Adam William Schoen

    (University of Maryland)

  • Vijay K. Tiwari

    (University of Maryland)

  • Ravi P. Singh

    (International Maize and Wheat Improvement Center (CIMMYT))

  • Mary J. Guttieri

    (Hard Winter Wheat Genetics Research Unit, USDA-ARS)

  • Harold N. Trick

    (Kansas State University)

  • Jesse Poland

    (Kansas State University
    King Abdullah University of Science and Technology (KAUST))

  • Robert L. Bowden

    (Hard Winter Wheat Genetics Research Unit, USDA-ARS)

  • Guihua Bai

    (Kansas State University
    Hard Winter Wheat Genetics Research Unit, USDA-ARS)

  • Bikram Gill

    (Kansas State University)

  • Sanzhen Liu

    (Kansas State University)

Abstract

The wheat wild relative Aegilops tauschii was previously used to transfer the Lr42 leaf rust resistance gene into bread wheat. Lr42 confers resistance at both seedling and adult stages, and it is broadly effective against all leaf rust races tested to date. Lr42 has been used extensively in the CIMMYT international wheat breeding program with resulting cultivars deployed in several countries. Here, using a bulked segregant RNA-Seq (BSR-Seq) mapping strategy, we identify three candidate genes for Lr42. Overexpression of a nucleotide-binding site leucine-rich repeat (NLR) gene AET1Gv20040300 induces strong resistance to leaf rust in wheat and a mutation of the gene disrupted the resistance. The Lr42 resistance allele is rare in Ae. tauschii and likely arose from ectopic recombination. Cloning of Lr42 provides diagnostic markers and over 1000 CIMMYT wheat lines carrying Lr42 have been developed documenting its widespread use and impact in crop improvement.

Suggested Citation

  • Guifang Lin & Hui Chen & Bin Tian & Sunish K. Sehgal & Lovepreet Singh & Jingzhong Xie & Nidhi Rawat & Philomin Juliana & Narinder Singh & Sandesh Shrestha & Duane L. Wilson & Hannah Shult & Hyeonju L, 2022. "Cloning of the broadly effective wheat leaf rust resistance gene Lr42 transferred from Aegilops tauschii," Nature Communications, Nature, vol. 13(1), pages 1-12, December.
    • Handle: RePEc:nat:natcom:v:13:y:2022:i:1:d:10.1038_s41467-022-30784-9
    DOI: 10.1038/s41467-022-30784-9
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    References listed on IDEAS

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    1. Hongna Li & Lei Hua & Shuqing Zhao & Ming Hao & Rui Song & Shuyong Pang & Yanna Liu & Hong Chen & Wenjun Zhang & Tao Shen & Jin-Ying Gou & Hailiang Mao & Guiping Wang & Xiaohua Hao & Jian Li & Baoxing, 2023. "Cloning of the wheat leaf rust resistance gene Lr47 introgressed from Aegilops speltoides," Nature Communications, Nature, vol. 14(1), pages 1-15, December.

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