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Wheat powdery mildew resistance gene Pm13 encodes a mixed lineage kinase domain-like protein

Author

Listed:
  • Huanhuan Li

    (Henan Agricultural University)

  • Wenqiang Men

    (Henan Agricultural University)

  • Chao Ma

    (Henan Agricultural University)

  • Qianwen Liu

    (Henan Agricultural University)

  • Zhenjie Dong

    (Nanjing Agricultural University)

  • Xiubin Tian

    (Chinese Academy of Sciences)

  • Chaoli Wang

    (Henan Agricultural University)

  • Cheng Liu

    (Shandong Academy of Agricultural Sciences)

  • Harsimardeep S. Gill

    (South Dakota State University)

  • Pengtao Ma

    (Yantai University)

  • Zhibin Zhang

    (Northeast Normal University)

  • Bao Liu

    (Northeast Normal University)

  • Yue Zhao

    (Henan Agricultural University)

  • Sunish K. Sehgal

    (South Dakota State University)

  • Wenxuan Liu

    (Henan Agricultural University)

Abstract

Wheat powdery mildew is one of the most destructive diseases threatening global wheat production. The wild relatives of wheat constitute rich sources of diversity for powdery mildew resistance. Here, we report the map-based cloning of the powdery mildew resistance gene Pm13 from the wild wheat species Aegilops longissima. Pm13 encodes a mixed lineage kinase domain-like (MLKL) protein that contains an N-terminal-domain of MLKL (MLKL_NTD) domain in its N-terminus and a C-terminal serine/threonine kinase (STK) domain. The resistance function of Pm13 is validated by mutagenesis, gene silencing, transgenic assay, and allelic association analyses. The development of introgression lines with significantly reduced chromosome segments of Ae. longissima encompassing Pm13 enables widespread deployment of this gene into wheat cultivars. The cloning of Pm13 may provide valuable insights into the molecular mechanisms underlying Pm13-mediated powdery mildew resistance and highlight the important roles of kinase fusion proteins (KFPs) in wheat immunity.

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  • Huanhuan Li & Wenqiang Men & Chao Ma & Qianwen Liu & Zhenjie Dong & Xiubin Tian & Chaoli Wang & Cheng Liu & Harsimardeep S. Gill & Pengtao Ma & Zhibin Zhang & Bao Liu & Yue Zhao & Sunish K. Sehgal & W, 2024. "Wheat powdery mildew resistance gene Pm13 encodes a mixed lineage kinase domain-like protein," Nature Communications, Nature, vol. 15(1), pages 1-13, December.
  • Handle: RePEc:nat:natcom:v:15:y:2024:i:1:d:10.1038_s41467-024-46814-7
    DOI: 10.1038/s41467-024-46814-7
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    as
    1. Sean Walkowiak & Liangliang Gao & Cecile Monat & Georg Haberer & Mulualem T. Kassa & Jemima Brinton & Ricardo H. Ramirez-Gonzalez & Markus C. Kolodziej & Emily Delorean & Dinushika Thambugala & Valent, 2020. "Multiple wheat genomes reveal global variation in modern breeding," Nature, Nature, vol. 588(7837), pages 277-283, December.
    2. Kathryn Tunyasuvunakool & Jonas Adler & Zachary Wu & Tim Green & Michal Zielinski & Augustin Žídek & Alex Bridgland & Andrew Cowie & Clemens Meyer & Agata Laydon & Sameer Velankar & Gerard J. Kleywegt, 2021. "Highly accurate protein structure prediction for the human proteome," Nature, Nature, vol. 596(7873), pages 590-596, August.
    3. Ping Lu & Li Guo & Zhenzhong Wang & Beibei Li & Jing Li & Yahui Li & Dan Qiu & Wenqi Shi & Lijun Yang & Ning Wang & Guanghao Guo & Jingzhong Xie & Qiuhong Wu & Yongxing Chen & Miaomiao Li & Huaizhi Zh, 2020. "A rare gain of function mutation in a wheat tandem kinase confers resistance to powdery mildew," Nature Communications, Nature, vol. 11(1), pages 1-11, December.
    4. John Jumper & Richard Evans & Alexander Pritzel & Tim Green & Michael Figurnov & Olaf Ronneberger & Kathryn Tunyasuvunakool & Russ Bates & Augustin Žídek & Anna Potapenko & Alex Bridgland & Clemens Me, 2021. "Highly accurate protein structure prediction with AlphaFold," Nature, Nature, vol. 596(7873), pages 583-589, August.
    5. Guotai Yu & Oadi Matny & Nicolas Champouret & Burkhard Steuernagel & Matthew J. Moscou & Inmaculada Hernández-Pinzón & Phon Green & Sadiye Hayta & Mark Smedley & Wendy Harwood & Ngonidzashe Kangara & , 2022. "Aegilops sharonensis genome-assisted identification of stem rust resistance gene Sr62," Nature Communications, Nature, vol. 13(1), pages 1-13, December.
    6. Valentina Klymiuk & Elitsur Yaniv & Lin Huang & Dina Raats & Andrii Fatiukha & Shisheng Chen & Lihua Feng & Zeev Frenkel & Tamar Krugman & Gabriel Lidzbarsky & Wei Chang & Marko J. Jääskeläinen & Chri, 2018. "Cloning of the wheat Yr15 resistance gene sheds light on the plant tandem kinase-pseudokinase family," Nature Communications, Nature, vol. 9(1), pages 1-12, December.
    7. Jonathan D. G. Jones & Jeffery L. Dangl, 2006. "The plant immune system," Nature, Nature, vol. 444(7117), pages 323-329, November.
    8. Fei Ni & Yanyan Zheng & Xiaoke Liu & Yang Yu & Guangqiang Zhang & Lynn Epstein & Xue Mao & Jingzheng Wu & Cuiling Yuan & Bo Lv & Haixia Yu & Jinlong Li & Qi Zhao & Qiyu Yang & Jiajun Liu & Juan Qi & D, 2023. "Sequencing trait-associated mutations to clone wheat rust-resistance gene YrNAM," Nature Communications, Nature, vol. 14(1), pages 1-8, December.
    9. Jianping Zhang & Timothy C. Hewitt & Willem H. P. Boshoff & Ian Dundas & Narayana Upadhyaya & Jianbo Li & Mehran Patpour & Sutha Chandramohan & Zacharias A. Pretorius & Mogens Hovmøller & Wendelin Sch, 2021. "A recombined Sr26 and Sr61 disease resistance gene stack in wheat encodes unrelated NLR genes," Nature Communications, Nature, vol. 12(1), pages 1-8, December.
    10. Hong-Qing Ling & Bin Ma & Xiaoli Shi & Hui Liu & Lingli Dong & Hua Sun & Yinghao Cao & Qiang Gao & Shusong Zheng & Ye Li & Ying Yu & Huilong Du & Ming Qi & Yan Li & Hongwei Lu & Hua Yu & Yan Cui & Nin, 2018. "Genome sequence of the progenitor of wheat A subgenome Triticum urartu," Nature, Nature, vol. 557(7705), pages 424-428, May.
    11. Hongna Li & Lei Hua & Shuqing Zhao & Ming Hao & Rui Song & Shuyong Pang & Yanna Liu & Hong Chen & Wenjun Zhang & Tao Shen & Jin-Ying Gou & Hailiang Mao & Guiping Wang & Xiaohua Hao & Jian Li & Baoxing, 2023. "Cloning of the wheat leaf rust resistance gene Lr47 introgressed from Aegilops speltoides," Nature Communications, Nature, vol. 14(1), pages 1-15, December.
    12. Jizeng Jia & Shancen Zhao & Xiuying Kong & Yingrui Li & Guangyao Zhao & Weiming He & Rudi Appels & Matthias Pfeifer & Yong Tao & Xueyong Zhang & Ruilian Jing & Chi Zhang & Youzhi Ma & Lifeng Gao & Chu, 2013. "Aegilops tauschii draft genome sequence reveals a gene repertoire for wheat adaptation," Nature, Nature, vol. 496(7443), pages 91-95, April.
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    Cited by:

    1. Miaomiao Li & Huaizhi Zhang & Huixin Xiao & Keyu Zhu & Wenqi Shi & Dong Zhang & Yong Wang & Lijun Yang & Qiuhong Wu & Jingzhong Xie & Yongxing Chen & Dan Qiu & Guanghao Guo & Ping Lu & Beibei Li & Lei, 2024. "A membrane associated tandem kinase from wild emmer wheat confers broad-spectrum resistance to powdery mildew," Nature Communications, Nature, vol. 15(1), pages 1-14, December.
    2. Yue Zhao & Zhenjie Dong & Jingnan Miao & Qianwen Liu & Chao Ma & Xiubin Tian & Jinqiu He & Huihui Bi & Wen Yao & Tao Li & Harsimardeep S. Gill & Zhibin Zhang & Aizhong Cao & Bao Liu & Huanhuan Li & Su, 2024. "Pm57 from Aegilops searsii encodes a tandem kinase protein and confers wheat powdery mildew resistance," Nature Communications, Nature, vol. 15(1), pages 1-13, December.
    3. Chao Ma & Xiubin Tian & Zhenjie Dong & Huanhuan Li & Xuexue Chen & Wenxuan Liu & Guihong Yin & Shuyang Ma & Liwei Zhang & Aizhong Cao & Cheng Liu & Hongfei Yan & Sunish K. Sehgal & Zhibin Zhang & Bao , 2024. "An Aegilops longissima NLR protein with integrated CC-BED module mediates resistance to wheat powdery mildew," Nature Communications, Nature, vol. 15(1), pages 1-13, December.
    4. Huagang He & Zhaozhao Chen & Renchun Fan & Jie Zhang & Shanying Zhu & Jiale Wang & Qianyuan Zhang & Anli Gao & Shuangjun Gong & Lu Zhang & Yanan Li & Yitong Zhao & Simon G. Krattinger & Qian-Hua Shen , 2024. "A kinase fusion protein from Aegilops longissima confers resistance to wheat powdery mildew," Nature Communications, Nature, vol. 15(1), pages 1-13, December.
    5. Davinder Sharma & Raz Avni & Juan Gutierrez-Gonzalez & Rakesh Kumar & Hanan Sela & Manas Ranjan Prusty & Arava Shatil-Cohen & István Molnár & Kateřina Holušová & Mahmoud Said & Jaroslav Doležel & Eita, 2024. "A single NLR gene confers resistance to leaf and stripe rust in wheat," Nature Communications, Nature, vol. 15(1), pages 1-16, December.

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