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Wheat powdery mildew resistance gene Pm13 encodes a mixed lineage kinase domain-like protein

Author

Listed:
  • Huanhuan Li

    (Henan Agricultural University)

  • Wenqiang Men

    (Henan Agricultural University)

  • Chao Ma

    (Henan Agricultural University)

  • Qianwen Liu

    (Henan Agricultural University)

  • Zhenjie Dong

    (Nanjing Agricultural University)

  • Xiubin Tian

    (Chinese Academy of Sciences)

  • Chaoli Wang

    (Henan Agricultural University)

  • Cheng Liu

    (Shandong Academy of Agricultural Sciences)

  • Harsimardeep S. Gill

    (South Dakota State University)

  • Pengtao Ma

    (Yantai University)

  • Zhibin Zhang

    (Northeast Normal University)

  • Bao Liu

    (Northeast Normal University)

  • Yue Zhao

    (Henan Agricultural University)

  • Sunish K. Sehgal

    (South Dakota State University)

  • Wenxuan Liu

    (Henan Agricultural University)

Abstract

Wheat powdery mildew is one of the most destructive diseases threatening global wheat production. The wild relatives of wheat constitute rich sources of diversity for powdery mildew resistance. Here, we report the map-based cloning of the powdery mildew resistance gene Pm13 from the wild wheat species Aegilops longissima. Pm13 encodes a mixed lineage kinase domain-like (MLKL) protein that contains an N-terminal-domain of MLKL (MLKL_NTD) domain in its N-terminus and a C-terminal serine/threonine kinase (STK) domain. The resistance function of Pm13 is validated by mutagenesis, gene silencing, transgenic assay, and allelic association analyses. The development of introgression lines with significantly reduced chromosome segments of Ae. longissima encompassing Pm13 enables widespread deployment of this gene into wheat cultivars. The cloning of Pm13 may provide valuable insights into the molecular mechanisms underlying Pm13-mediated powdery mildew resistance and highlight the important roles of kinase fusion proteins (KFPs) in wheat immunity.

Suggested Citation

  • Huanhuan Li & Wenqiang Men & Chao Ma & Qianwen Liu & Zhenjie Dong & Xiubin Tian & Chaoli Wang & Cheng Liu & Harsimardeep S. Gill & Pengtao Ma & Zhibin Zhang & Bao Liu & Yue Zhao & Sunish K. Sehgal & W, 2024. "Wheat powdery mildew resistance gene Pm13 encodes a mixed lineage kinase domain-like protein," Nature Communications, Nature, vol. 15(1), pages 1-13, December.
    • Handle: RePEc:nat:natcom:v:15:y:2024:i:1:d:10.1038_s41467-024-46814-7
    DOI: 10.1038/s41467-024-46814-7
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    1. Miaomiao Li & Huaizhi Zhang & Huixin Xiao & Keyu Zhu & Wenqi Shi & Dong Zhang & Yong Wang & Lijun Yang & Qiuhong Wu & Jingzhong Xie & Yongxing Chen & Dan Qiu & Guanghao Guo & Ping Lu & Beibei Li & Lei, 2024. "A membrane associated tandem kinase from wild emmer wheat confers broad-spectrum resistance to powdery mildew," Nature Communications, Nature, vol. 15(1), pages 1-14, December.

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