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Sequencing trait-associated mutations to clone wheat rust-resistance gene YrNAM

Author

Listed:
  • Fei Ni

    (Shandong Agricultural University)

  • Yanyan Zheng

    (Shandong Agricultural University
    Zhoucun District Agricultural Technology Service Center)

  • Xiaoke Liu

    (Shandong Agricultural University)

  • Yang Yu

    (Shandong Agricultural University)

  • Guangqiang Zhang

    (Shandong Agricultural University
    Heze University)

  • Lynn Epstein

    (University of California)

  • Xue Mao

    (Shandong Agricultural University)

  • Jingzheng Wu

    (Shandong Agricultural University
    Zhejiang Pharmaceutical University)

  • Cuiling Yuan

    (Shandong Agricultural University
    Shandong Peanut Research Institute)

  • Bo Lv

    (Shandong Agricultural University)

  • Haixia Yu

    (Shandong Agricultural University)

  • Jinlong Li

    (Shandong Agricultural University
    China Agricultural University)

  • Qi Zhao

    (Shandong Agricultural University)

  • Qiyu Yang

    (Shandong Agricultural University)

  • Jiajun Liu

    (Shandong Agricultural University)

  • Juan Qi

    (Shandong Agricultural University)

  • Daolin Fu

    (Shandong Agricultural University
    Spring Valley Agriscience Co., Ltd.)

  • Jiajie Wu

    (Shandong Agricultural University)

Abstract

Stripe (yellow) rust, caused by Puccinia striiformis f. sp. tritici (Pst), can significantly affect wheat production. Cloning resistance genes is critical for efficient and effective breeding of stripe rust resistant wheat cultivars. One resistance gene (Yr10CG) underlying the Pst resistance locus Yr10 has been cloned. However, following haplotype and linkage analyses indicate the presence of additional Pst resistance gene(s) underlying/near Yr10 locus. Here, we report the cloning of the Pst resistance gene YrNAM in this region using the method of sequencing trait-associated mutations (STAM). YrNAM encodes a non-canonical resistance protein with a NAM domain and a ZnF-BED domain. We show that both domains are required for resistance. Transgenic wheat harboring YrNAM gene driven by its endogenous promoter confers resistance to stripe rust races CYR32 and CYR33. YrNAM is an ancient gene and present in wild wheat species Aegilops longissima and Ae. sharonensis; however, it is absent in most wheat cultivars, which indicates its breeding value.

Suggested Citation

  • Fei Ni & Yanyan Zheng & Xiaoke Liu & Yang Yu & Guangqiang Zhang & Lynn Epstein & Xue Mao & Jingzheng Wu & Cuiling Yuan & Bo Lv & Haixia Yu & Jinlong Li & Qi Zhao & Qiyu Yang & Jiajun Liu & Juan Qi & D, 2023. "Sequencing trait-associated mutations to clone wheat rust-resistance gene YrNAM," Nature Communications, Nature, vol. 14(1), pages 1-8, December.
    • Handle: RePEc:nat:natcom:v:14:y:2023:i:1:d:10.1038_s41467-023-39993-2
    DOI: 10.1038/s41467-023-39993-2
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    References listed on IDEAS

    as
    1. Jianping Zhang & Timothy C. Hewitt & Willem H. P. Boshoff & Ian Dundas & Narayana Upadhyaya & Jianbo Li & Mehran Patpour & Sutha Chandramohan & Zacharias A. Pretorius & Mogens Hovmøller & Wendelin Sch, 2021. "A recombined Sr26 and Sr61 disease resistance gene stack in wheat encodes unrelated NLR genes," Nature Communications, Nature, vol. 12(1), pages 1-8, December.
    2. Guotai Yu & Oadi Matny & Nicolas Champouret & Burkhard Steuernagel & Matthew J. Moscou & Inmaculada Hernández-Pinzón & Phon Green & Sadiye Hayta & Mark Smedley & Wendy Harwood & Ngonidzashe Kangara & , 2022. "Aegilops sharonensis genome-assisted identification of stem rust resistance gene Sr62," Nature Communications, Nature, vol. 13(1), pages 1-13, December.
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    Cited by:

    1. Katherine Dibley & Matthias Jost & Robert McIntosh & Evans Lagudah & Peng Zhang, 2024. "The wheat stripe rust resistance gene YrNAM is Yr10," Nature Communications, Nature, vol. 15(1), pages 1-3, December.
    2. Chao Ma & Xiubin Tian & Zhenjie Dong & Huanhuan Li & Xuexue Chen & Wenxuan Liu & Guihong Yin & Shuyang Ma & Liwei Zhang & Aizhong Cao & Cheng Liu & Hongfei Yan & Sunish K. Sehgal & Zhibin Zhang & Bao , 2024. "An Aegilops longissima NLR protein with integrated CC-BED module mediates resistance to wheat powdery mildew," Nature Communications, Nature, vol. 15(1), pages 1-13, December.
    3. Huagang He & Zhaozhao Chen & Renchun Fan & Jie Zhang & Shanying Zhu & Jiale Wang & Qianyuan Zhang & Anli Gao & Shuangjun Gong & Lu Zhang & Yanan Li & Yitong Zhao & Simon G. Krattinger & Qian-Hua Shen , 2024. "A kinase fusion protein from Aegilops longissima confers resistance to wheat powdery mildew," Nature Communications, Nature, vol. 15(1), pages 1-13, December.
    4. Huanhuan Li & Wenqiang Men & Chao Ma & Qianwen Liu & Zhenjie Dong & Xiubin Tian & Chaoli Wang & Cheng Liu & Harsimardeep S. Gill & Pengtao Ma & Zhibin Zhang & Bao Liu & Yue Zhao & Sunish K. Sehgal & W, 2024. "Wheat powdery mildew resistance gene Pm13 encodes a mixed lineage kinase domain-like protein," Nature Communications, Nature, vol. 15(1), pages 1-13, December.

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