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A pathogen-induced putative NAC transcription factor mediates leaf rust resistance in barley

Author

Listed:
  • Chunhong Chen

    (Commonwealth Scientific and Industrial Research Organisation)

  • Matthias Jost

    (Commonwealth Scientific and Industrial Research Organisation)

  • Megan A. Outram

    (Commonwealth Scientific and Industrial Research Organisation)

  • Dorian Friendship

    (The University of Sydney, Faculty of Science, Plant Breeding Institute)

  • Jian Chen

    (Commonwealth Scientific and Industrial Research Organisation)

  • Aihua Wang

    (Commonwealth Scientific and Industrial Research Organisation)

  • Sambasivam Periyannan

    (Commonwealth Scientific and Industrial Research Organisation
    The University of Southern Queensland, School of Agriculture and Environmental Science, Centre for Crop Health)

  • Jan Bartoš

    (Centre of Plant Structural and Functional Genomics)

  • Kateřina Holušová

    (Centre of Plant Structural and Functional Genomics)

  • Jaroslav Doležel

    (Centre of Plant Structural and Functional Genomics)

  • Peng Zhang

    (The University of Sydney, Faculty of Science, Plant Breeding Institute)

  • Dhara Bhatt

    (Commonwealth Scientific and Industrial Research Organisation)

  • Davinder Singh

    (The University of Sydney, Faculty of Science, Plant Breeding Institute)

  • Evans Lagudah

    (Commonwealth Scientific and Industrial Research Organisation)

  • Robert F. Park

    (The University of Sydney, Faculty of Science, Plant Breeding Institute)

  • Peter M. Dracatos

    (The University of Sydney, Faculty of Science, Plant Breeding Institute
    La Trobe University)

Abstract

Leaf rust, caused by Puccinia hordei, is one of the most widespread and damaging foliar diseases affecting barley. The barley leaf rust resistance locus Rph7 has been shown to have unusually high sequence and haplotype divergence. In this study, we isolate the Rph7 gene using a fine mapping and RNA-Seq approach that is confirmed by mutational analysis and transgenic complementation. Rph7 is a pathogen-induced, non-canonical resistance gene encoding a protein that is distinct from other known plant disease resistance proteins in the Triticeae. Structural analysis using an AlphaFold2 protein model suggests that Rph7 encodes a putative NAC transcription factor with a zinc-finger BED domain with structural similarity to the N-terminal DNA-binding domain of the NAC transcription factor (ANAC019) from Arabidopsis. A global gene expression analysis suggests Rph7 mediates the activation and strength of the basal defence response. The isolation of Rph7 highlights the diversification of resistance mechanisms available for engineering disease control in crops.

Suggested Citation

  • Chunhong Chen & Matthias Jost & Megan A. Outram & Dorian Friendship & Jian Chen & Aihua Wang & Sambasivam Periyannan & Jan Bartoš & Kateřina Holušová & Jaroslav Doležel & Peng Zhang & Dhara Bhatt & Da, 2023. "A pathogen-induced putative NAC transcription factor mediates leaf rust resistance in barley," Nature Communications, Nature, vol. 14(1), pages 1-11, December.
    • Handle: RePEc:nat:natcom:v:14:y:2023:i:1:d:10.1038_s41467-023-41021-2
    DOI: 10.1038/s41467-023-41021-2
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    References listed on IDEAS

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    1. Sean Walkowiak & Liangliang Gao & Cecile Monat & Georg Haberer & Mulualem T. Kassa & Jemima Brinton & Ricardo H. Ramirez-Gonzalez & Markus C. Kolodziej & Emily Delorean & Dinushika Thambugala & Valent, 2020. "Multiple wheat genomes reveal global variation in modern breeding," Nature, Nature, vol. 588(7837), pages 277-283, December.
    2. Murukarthick Jayakodi & Sudharsan Padmarasu & Georg Haberer & Venkata Suresh Bonthala & Heidrun Gundlach & Cécile Monat & Thomas Lux & Nadia Kamal & Daniel Lang & Axel Himmelbach & Jennifer Ens & Xiao, 2020. "The barley pan-genome reveals the hidden legacy of mutation breeding," Nature, Nature, vol. 588(7837), pages 284-289, December.
    3. Hoan X. Dinh & Davinder Singh & Diana Gomez de la Cruz & Goetz Hensel & Jochen Kumlehn & Martin Mascher & Nils Stein & Dragan Perovic & Michael Ayliffe & Matthew J. Moscou & Robert F. Park & Mohammad , 2022. "The barley leaf rust resistance gene Rph3 encodes a predicted membrane protein and is induced upon infection by avirulent pathotypes of Puccinia hordei," Nature Communications, Nature, vol. 13(1), pages 1-13, December.
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    Cited by:

    1. Katherine Dibley & Matthias Jost & Robert McIntosh & Evans Lagudah & Peng Zhang, 2024. "The wheat stripe rust resistance gene YrNAM is Yr10," Nature Communications, Nature, vol. 15(1), pages 1-3, December.

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