IDEAS home Printed from https://ideas.repec.org/a/nat/natcom/v14y2023i1d10.1038_s41467-023-41021-2.html
   My bibliography  Save this article

A pathogen-induced putative NAC transcription factor mediates leaf rust resistance in barley

Author

Listed:
  • Chunhong Chen

    (Commonwealth Scientific and Industrial Research Organisation)

  • Matthias Jost

    (Commonwealth Scientific and Industrial Research Organisation)

  • Megan A. Outram

    (Commonwealth Scientific and Industrial Research Organisation)

  • Dorian Friendship

    (The University of Sydney, Faculty of Science, Plant Breeding Institute)

  • Jian Chen

    (Commonwealth Scientific and Industrial Research Organisation)

  • Aihua Wang

    (Commonwealth Scientific and Industrial Research Organisation)

  • Sambasivam Periyannan

    (Commonwealth Scientific and Industrial Research Organisation
    The University of Southern Queensland, School of Agriculture and Environmental Science, Centre for Crop Health)

  • Jan Bartoš

    (Centre of Plant Structural and Functional Genomics)

  • Kateřina Holušová

    (Centre of Plant Structural and Functional Genomics)

  • Jaroslav Doležel

    (Centre of Plant Structural and Functional Genomics)

  • Peng Zhang

    (The University of Sydney, Faculty of Science, Plant Breeding Institute)

  • Dhara Bhatt

    (Commonwealth Scientific and Industrial Research Organisation)

  • Davinder Singh

    (The University of Sydney, Faculty of Science, Plant Breeding Institute)

  • Evans Lagudah

    (Commonwealth Scientific and Industrial Research Organisation)

  • Robert F. Park

    (The University of Sydney, Faculty of Science, Plant Breeding Institute)

  • Peter M. Dracatos

    (The University of Sydney, Faculty of Science, Plant Breeding Institute
    La Trobe University)

Abstract

Leaf rust, caused by Puccinia hordei, is one of the most widespread and damaging foliar diseases affecting barley. The barley leaf rust resistance locus Rph7 has been shown to have unusually high sequence and haplotype divergence. In this study, we isolate the Rph7 gene using a fine mapping and RNA-Seq approach that is confirmed by mutational analysis and transgenic complementation. Rph7 is a pathogen-induced, non-canonical resistance gene encoding a protein that is distinct from other known plant disease resistance proteins in the Triticeae. Structural analysis using an AlphaFold2 protein model suggests that Rph7 encodes a putative NAC transcription factor with a zinc-finger BED domain with structural similarity to the N-terminal DNA-binding domain of the NAC transcription factor (ANAC019) from Arabidopsis. A global gene expression analysis suggests Rph7 mediates the activation and strength of the basal defence response. The isolation of Rph7 highlights the diversification of resistance mechanisms available for engineering disease control in crops.

Suggested Citation

  • Chunhong Chen & Matthias Jost & Megan A. Outram & Dorian Friendship & Jian Chen & Aihua Wang & Sambasivam Periyannan & Jan Bartoš & Kateřina Holušová & Jaroslav Doležel & Peng Zhang & Dhara Bhatt & Da, 2023. "A pathogen-induced putative NAC transcription factor mediates leaf rust resistance in barley," Nature Communications, Nature, vol. 14(1), pages 1-11, December.
    • Handle: RePEc:nat:natcom:v:14:y:2023:i:1:d:10.1038_s41467-023-41021-2
    DOI: 10.1038/s41467-023-41021-2
    as

    Download full text from publisher

    File URL: https://www.nature.com/articles/s41467-023-41021-2
    File Function: Abstract
    Download Restriction: no
    File URL: https://libkey.io/10.1038/s41467-023-41021-2?utm_source=ideas
    LibKey link: if access is restricted and if your library uses this service, LibKey will redirect you to where you can use your library subscription to access this item
    ---><---

    References listed on IDEAS

    as
    1. Sean Walkowiak & Liangliang Gao & Cecile Monat & Georg Haberer & Mulualem T. Kassa & Jemima Brinton & Ricardo H. Ramirez-Gonzalez & Markus C. Kolodziej & Emily Delorean & Dinushika Thambugala & Valent, 2020. "Multiple wheat genomes reveal global variation in modern breeding," Nature, Nature, vol. 588(7837), pages 277-283, December.
    2. Murukarthick Jayakodi & Sudharsan Padmarasu & Georg Haberer & Venkata Suresh Bonthala & Heidrun Gundlach & Cécile Monat & Thomas Lux & Nadia Kamal & Daniel Lang & Axel Himmelbach & Jennifer Ens & Xiao, 2020. "The barley pan-genome reveals the hidden legacy of mutation breeding," Nature, Nature, vol. 588(7837), pages 284-289, December.
    3. Hoan X. Dinh & Davinder Singh & Diana Gomez de la Cruz & Goetz Hensel & Jochen Kumlehn & Martin Mascher & Nils Stein & Dragan Perovic & Michael Ayliffe & Matthew J. Moscou & Robert F. Park & Mohammad , 2022. "The barley leaf rust resistance gene Rph3 encodes a predicted membrane protein and is induced upon infection by avirulent pathotypes of Puccinia hordei," Nature Communications, Nature, vol. 13(1), pages 1-13, December.
    Full references (including those not matched with items on IDEAS)

    Citations

    Citations are extracted by the CitEc Project, subscribe to its RSS feed for this item.
    as


    Cited by:

    1. Katherine Dibley & Matthias Jost & Robert McIntosh & Evans Lagudah & Peng Zhang, 2024. "The wheat stripe rust resistance gene YrNAM is Yr10," Nature Communications, Nature, vol. 15(1), pages 1-3, December.

    Most related items

    These are the items that most often cite the same works as this one and are cited by the same works as this one.
    1. Miaomiao Li & Huaizhi Zhang & Huixin Xiao & Keyu Zhu & Wenqi Shi & Dong Zhang & Yong Wang & Lijun Yang & Qiuhong Wu & Jingzhong Xie & Yongxing Chen & Dan Qiu & Guanghao Guo & Ping Lu & Beibei Li & Lei, 2024. "A membrane associated tandem kinase from wild emmer wheat confers broad-spectrum resistance to powdery mildew," Nature Communications, Nature, vol. 15(1), pages 1-14, December.
    2. Sylvain Aubry, 2023. "Genebanking plant genetic resources in the postgenomic era," Agriculture and Human Values, Springer;The Agriculture, Food, & Human Values Society (AFHVS), vol. 40(3), pages 961-971, September.
    3. Dongya Wu & Enhui Shen & Bowen Jiang & Yu Feng & Wei Tang & Sangting Lao & Lei Jia & Han-Yang Lin & Lingjuan Xie & Xifang Weng & Chenfeng Dong & Qinghong Qian & Feng Lin & Haiming Xu & Huabing Lu & Lu, 2022. "Genomic insights into the evolution of Echinochloa species as weed and orphan crop," Nature Communications, Nature, vol. 13(1), pages 1-16, December.
    4. Huanhuan Li & Wenqiang Men & Chao Ma & Qianwen Liu & Zhenjie Dong & Xiubin Tian & Chaoli Wang & Cheng Liu & Harsimardeep S. Gill & Pengtao Ma & Zhibin Zhang & Bao Liu & Yue Zhao & Sunish K. Sehgal & W, 2024. "Wheat powdery mildew resistance gene Pm13 encodes a mixed lineage kinase domain-like protein," Nature Communications, Nature, vol. 15(1), pages 1-13, December.
    5. Heiner Kuhl & Kang Du & Manfred Schartl & Lukáš Kalous & Matthias Stöck & Dunja K. Lamatsch, 2022. "Equilibrated evolution of the mixed auto-/allopolyploid haplotype-resolved genome of the invasive hexaploid Prussian carp," Nature Communications, Nature, vol. 13(1), pages 1-11, December.
    6. Zihao Wang & Wenxi Wang & Xiaoming Xie & Yongfa Wang & Zhengzhao Yang & Huiru Peng & Mingming Xin & Yingyin Yao & Zhaorong Hu & Jie Liu & Zhenqi Su & Chaojie Xie & Baoyun Li & Zhongfu Ni & Qixin Sun &, 2022. "Dispersed emergence and protracted domestication of polyploid wheat uncovered by mosaic ancestral haploblock inference," Nature Communications, Nature, vol. 13(1), pages 1-14, December.
    7. Linsan Liu & Sarah B. Jose & Chiara Campoli & Micha M. Bayer & Miguel A. Sánchez-Diaz & Trisha McAllister & Yichun Zhou & Mhmoud Eskan & Linda Milne & Miriam Schreiber & Thomas Batstone & Ian D. Bull , 2022. "Conserved signalling components coordinate epidermal patterning and cuticle deposition in barley," Nature Communications, Nature, vol. 13(1), pages 1-18, December.
    8. Fei He & Wei Wang & William B. Rutter & Katherine W. Jordan & Jie Ren & Ellie Taagen & Noah DeWitt & Deepmala Sehgal & Sivakumar Sukumaran & Susanne Dreisigacker & Matthew Reynolds & Jyotirmoy Halder , 2022. "Genomic variants affecting homoeologous gene expression dosage contribute to agronomic trait variation in allopolyploid wheat," Nature Communications, Nature, vol. 13(1), pages 1-15, December.
    9. Jianping Zhang & Jayaveeramuthu Nirmala & Shisheng Chen & Matthias Jost & Burkhard Steuernagel & Mirka Karafiatova & Tim Hewitt & Hongna Li & Erena Edae & Keshav Sharma & Sami Hoxha & Dhara Bhatt & Re, 2023. "Single amino acid change alters specificity of the multi-allelic wheat stem rust resistance locus SR9," Nature Communications, Nature, vol. 14(1), pages 1-12, December.
    10. Xiaohua Yao & Leping Su & Youhua Yao & Likun An & Yixiong Bai & Xin Li & Kunlun Wu, 2022. "Isolation, sequencing of the HvnHID gene and its role in the purple-grain colour development in Tibetan hulless barley," Czech Journal of Genetics and Plant Breeding, Czech Academy of Agricultural Sciences, vol. 58(1), pages 1-9.
    11. Habteab Goitom Gebremedhin & Yahui Li & Jinghuang Hu & Dan Qiu & Qiuhong Wu & Hongjun Zhang & Li Yang & Yang Zhou & Yijun Zhou & Zhiyong Liu & Peng Zhang & Hongjie Li, 2022. "Development of KASP and SSR Markers for PmQ , a Recessive Gene Conferring Powdery Mildew Resistance in Wheat Landrace Qingxinmai," Agriculture, MDPI, vol. 12(9), pages 1-10, August.
    12. Junhui Yuan & Sanjie Jiang & Jianbo Jian & Mingyu Liu & Zhen Yue & Jiabao Xu & Juan Li & Chunyan Xu & Lihong Lin & Yi Jing & Xiaoxiao Zhang & Haixin Chen & Linjuan Zhang & Tao Fu & Shuiyan Yu & Zhangy, 2022. "Genomic basis of the giga-chromosomes and giga-genome of tree peony Paeonia ostii," Nature Communications, Nature, vol. 13(1), pages 1-16, December.
    13. Ting Wang & Shiyao Duan & Chen Xu & Yi Wang & Xinzhong Zhang & Xuefeng Xu & Liyang Chen & Zhenhai Han & Ting Wu, 2023. "Pan-genome analysis of 13 Malus accessions reveals structural and sequence variations associated with fruit traits," Nature Communications, Nature, vol. 14(1), pages 1-15, December.
    14. Tuomas Hämälä & Christopher Moore & Laura Cowan & Matthew Carlile & David Gopaulchan & Marie K. Brandrud & Siri Birkeland & Matthew Loose & Filip Kolář & Marcus A. Koch & Levi Yant, 2024. "Impact of whole-genome duplications on structural variant evolution in Cochlearia," Nature Communications, Nature, vol. 15(1), pages 1-13, December.
    15. Hoan X. Dinh & Davinder Singh & Diana Gomez de la Cruz & Goetz Hensel & Jochen Kumlehn & Martin Mascher & Nils Stein & Dragan Perovic & Michael Ayliffe & Matthew J. Moscou & Robert F. Park & Mohammad , 2022. "The barley leaf rust resistance gene Rph3 encodes a predicted membrane protein and is induced upon infection by avirulent pathotypes of Puccinia hordei," Nature Communications, Nature, vol. 13(1), pages 1-13, December.
    16. Salma O. M. Osman & Abu Sefyan I. Saad & Shota Tadano & Yoshiki Takeda & Yuji Yamasaki & Izzat S. A. Tahir & Hisashi Tsujimoto & Kinya Akashi, 2022. "Probing Differential Metabolome Responses among Wheat Genotypes to Heat Stress Using Fourier Transform Infrared-Based Chemical Fingerprinting," Agriculture, MDPI, vol. 12(6), pages 1-14, May.
    17. Gaia Cortinovis & Leonardo Vincenzi & Robyn Anderson & Giovanni Marturano & Jacob Ian Marsh & Philipp Emanuel Bayer & Lorenzo Rocchetti & Giulia Frascarelli & Giovanna Lanzavecchia & Alice Pieri & And, 2024. "Adaptive gene loss in the common bean pan-genome during range expansion and domestication," Nature Communications, Nature, vol. 15(1), pages 1-15, December.
    18. Liqiang Song & Ruihui Wang & Xueju Yang & Aimin Zhang & Dongcheng Liu, 2023. "Molecular Markers and Their Applications in Marker-Assisted Selection (MAS) in Bread Wheat ( Triticum aestivum L.)," Agriculture, MDPI, vol. 13(3), pages 1-18, March.
    19. Carmen Escudero-Martinez & Max Coulter & Rodrigo Alegria Terrazas & Alexandre Foito & Rumana Kapadia & Laura Pietrangelo & Mauro Maver & Rajiv Sharma & Alessio Aprile & Jenny Morris & Pete E. Hedley &, 2022. "Identifying plant genes shaping microbiota composition in the barley rhizosphere," Nature Communications, Nature, vol. 13(1), pages 1-14, December.

    More about this item

    Statistics

    Access and download statistics

    Corrections

    All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:nat:natcom:v:14:y:2023:i:1:d:10.1038_s41467-023-41021-2. See general information about how to correct material in RePEc.

    If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.

    If CitEc recognized a bibliographic reference but did not link an item in RePEc to it, you can help with this form .

    If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.

    For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: Sonal Shukla or Springer Nature Abstracting and Indexing (email available below). General contact details of provider: http://www.nature.com .

    Please note that corrections may take a couple of weeks to filter through the various RePEc services.

    IDEAS is a RePEc service. RePEc uses bibliographic data supplied by the respective publishers.