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The barley leaf rust resistance gene Rph3 encodes a predicted membrane protein and is induced upon infection by avirulent pathotypes of Puccinia hordei

Author

Listed:
  • Hoan X. Dinh

    (The University of Sydney, Faculty of Science, Plant Breeding Institute)

  • Davinder Singh

    (The University of Sydney, Faculty of Science, Plant Breeding Institute)

  • Diana Gomez de la Cruz

    (The Sainsbury Laboratory, University of East Anglia, Norwich Research Park)

  • Goetz Hensel

    (Leibniz Institute of Plant Genetics and Crop Plant Research (IPK))

  • Jochen Kumlehn

    (Leibniz Institute of Plant Genetics and Crop Plant Research (IPK))

  • Martin Mascher

    (Leibniz Institute of Plant Genetics and Crop Plant Research (IPK)
    German Centre for Integrative Biodiversity Research (iDiv) Halle-Jena-Leipzig)

  • Nils Stein

    (Leibniz Institute of Plant Genetics and Crop Plant Research (IPK)
    Center of integrated Breeding Research (CiBreed), Department of Crop Sciences, Georg-August-University)

  • Dragan Perovic

    (Julius Kühn-Institut, Institute for Resistance Research and Stress Tolerance)

  • Michael Ayliffe

    (Commonwealth Scientific and Industrial Research Organisation)

  • Matthew J. Moscou

    (The Sainsbury Laboratory, University of East Anglia, Norwich Research Park)

  • Robert F. Park

    (The University of Sydney, Faculty of Science, Plant Breeding Institute)

  • Mohammad Pourkheirandish

    (The University of Melbourne, Faculty of Veterinary and Agriculture)

Abstract

Leaf rust, caused by Puccinia hordei, is an economically significant disease of barley, but only a few major resistance genes to P. hordei (Rph) have been cloned. In this study, gene Rph3 was isolated by positional cloning and confirmed by mutational analysis and transgenic complementation. The Rph3 gene, which originated from wild barley and was first introgressed into cultivated Egyptian germplasm, encodes a unique predicted transmembrane resistance protein that differs from all known plant disease resistance proteins at the amino acid sequence level. Genetic profiles of diverse accessions indicated limited genetic diversity in Rph3 in domesticated germplasm, and higher diversity in wild barley from the Eastern Mediterranean region. The Rph3 gene was expressed only in interactions with Rph3-avirulent P. hordei isolates, a phenomenon also observed for transcription activator-like effector-dependent genes known as executors conferring resistance to Xanthomonas spp. Like known transmembrane executors such as Bs3 and Xa7, heterologous expression of Rph3 in N. benthamiana induced a cell death response. The isolation of Rph3 highlights convergent evolutionary processes in diverse plant-pathogen interaction systems, where similar defence mechanisms evolved independently in monocots and dicots.

Suggested Citation

  • Hoan X. Dinh & Davinder Singh & Diana Gomez de la Cruz & Goetz Hensel & Jochen Kumlehn & Martin Mascher & Nils Stein & Dragan Perovic & Michael Ayliffe & Matthew J. Moscou & Robert F. Park & Mohammad , 2022. "The barley leaf rust resistance gene Rph3 encodes a predicted membrane protein and is induced upon infection by avirulent pathotypes of Puccinia hordei," Nature Communications, Nature, vol. 13(1), pages 1-13, December.
    • Handle: RePEc:nat:natcom:v:13:y:2022:i:1:d:10.1038_s41467-022-29840-1
    DOI: 10.1038/s41467-022-29840-1
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    References listed on IDEAS

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    Cited by:

    1. Miaomiao Li & Huaizhi Zhang & Huixin Xiao & Keyu Zhu & Wenqi Shi & Dong Zhang & Yong Wang & Lijun Yang & Qiuhong Wu & Jingzhong Xie & Yongxing Chen & Dan Qiu & Guanghao Guo & Ping Lu & Beibei Li & Lei, 2024. "A membrane associated tandem kinase from wild emmer wheat confers broad-spectrum resistance to powdery mildew," Nature Communications, Nature, vol. 15(1), pages 1-14, December.
    2. Chunhong Chen & Matthias Jost & Megan A. Outram & Dorian Friendship & Jian Chen & Aihua Wang & Sambasivam Periyannan & Jan Bartoš & Kateřina Holušová & Jaroslav Doležel & Peng Zhang & Dhara Bhatt & Da, 2023. "A pathogen-induced putative NAC transcription factor mediates leaf rust resistance in barley," Nature Communications, Nature, vol. 14(1), pages 1-11, December.

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