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One-step direct transesterification of wet yeast for biodiesel production catalyzed by magnetic nanoparticle-immobilized lipase

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  • Cao, Xiyue
  • Xu, Hui
  • Li, Fosheng
  • Zou, Yijun
  • Ran, Yulu
  • Ma, Xiaorui
  • Cao, Yu
  • Xu, Qingrui
  • Qiao, Dairong
  • Cao, Yi

Abstract

To develop a method for direct transesterification of wet yeast using immobilized lipase, the oleaginous yeast Saitozyma podzolica Zwy-2-3 and the lipase producing Burkholderia pyrrolica WZ10-3 were used as materials for production of biodiesel. Fe3O4@SiO2-CHO prepared by modifying Fe3O4 with TEOS, APTES and glutaraldehyde. The biocatalysts covalently cross-linked with WZ10-3 lipase by Fe3O4@SiO2-CHO were characterized by FTIR, XRD and TEM. When the enzyme dosage, glutaraldehyde concentration, temperature and time were 30.22 mL, 2.0%, 40 °C and 4 h, the immobilized lipase activity and immobilization rate reached 10038.0 U/g and 96.9%, respectively. The optimum temperatures for immobilized and free lipase were 60 °C and 40 °C. The immobilized enzyme still had 80% enzymatic activity after 48 d storage at 4 °C. The optimized conditions for the direct conversion of immobilized lipase to esterified wet yeast (one-step) were: enzyme dosage 2.5 g, reaction temperature 35 °C; water content 15%; and molar ratio of n-hexane to methanol 3: 1. The transesterification rates of one-step method for oil and biomass were 98.12% and 56.11%, respectively. In contrast, the two-step method was only 88.75% and 51.21%. The immobilized enzyme had 90% enzyme activity after 10 times of reuse.

Suggested Citation

  • Cao, Xiyue & Xu, Hui & Li, Fosheng & Zou, Yijun & Ran, Yulu & Ma, Xiaorui & Cao, Yu & Xu, Qingrui & Qiao, Dairong & Cao, Yi, 2021. "One-step direct transesterification of wet yeast for biodiesel production catalyzed by magnetic nanoparticle-immobilized lipase," Renewable Energy, Elsevier, vol. 171(C), pages 11-21.
    • Handle: RePEc:eee:renene:v:171:y:2021:i:c:p:11-21
    DOI: 10.1016/j.renene.2021.02.065
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    1. Aboelazayem, Omar & Gadalla, Mamdouh & Alhajri, Ibrahim & Saha, Basudeb, 2021. "Advanced process integration for supercritical production of biodiesel: Residual waste heat recovery via organic Rankine cycle (ORC)," Renewable Energy, Elsevier, vol. 164(C), pages 433-443.
    2. Gog, Adriana & Roman, Marius & Toşa, Monica & Paizs, Csaba & Irimie, Florin Dan, 2012. "Biodiesel production using enzymatic transesterification – Current state and perspectives," Renewable Energy, Elsevier, vol. 39(1), pages 10-16.
    3. Liu, Chien-Hung & Huang, Chien-Chang & Wang, Yao-Wen & Lee, Duu-Jong & Chang, Jo-Shu, 2012. "Biodiesel production by enzymatic transesterification catalyzed by Burkholderia lipase immobilized on hydrophobic magnetic particles," Applied Energy, Elsevier, vol. 100(C), pages 41-46.
    4. Goh, Brandon Han Hoe & Ong, Hwai Chyuan & Cheah, Mei Yee & Chen, Wei-Hsin & Yu, Kai Ling & Mahlia, Teuku Meurah Indra, 2019. "Sustainability of direct biodiesel synthesis from microalgae biomass: A critical review," Renewable and Sustainable Energy Reviews, Elsevier, vol. 107(C), pages 59-74.
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    1. Aghel, Babak & Biabani, Arash, 2024. "Using solar microreactors and photocatalysts to synthesize biodiesel," Renewable Energy, Elsevier, vol. 220(C).
    2. Guilherme, Ederson Paulo Xavier & Zanphorlin, Leticia Maria & Sousa, Amanda Silva & Miyamoto, Renan Yuji & Bruziquesi, Carlos Giovani Oliveira & Mesquita, Bruna Mara Aparecida de Carvalho & Santos, Se, 2022. "Simultaneous saccharification isomerization and Co-fermentation – SSICF: A new process concept for second-generation ethanol biorefineries combining immobilized recombinant enzymes and non-GMO Sacchar," Renewable Energy, Elsevier, vol. 182(C), pages 274-284.
    3. Wancura, João H.C. & Brondani, Michel & dos Santos, Maicon S.N. & Oro, Carolina E.D. & Wancura, Guilherme C. & Tres, Marcus V. & Oliveira, J. Vladimir, 2023. "Demystifying the enzymatic biodiesel: How lipases are contributing to its technological advances," Renewable Energy, Elsevier, vol. 216(C).

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