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Simultaneous saccharification isomerization and Co-fermentation – SSICF: A new process concept for second-generation ethanol biorefineries combining immobilized recombinant enzymes and non-GMO Saccharomyces

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  • Guilherme, Ederson Paulo Xavier
  • Zanphorlin, Leticia Maria
  • Sousa, Amanda Silva
  • Miyamoto, Renan Yuji
  • Bruziquesi, Carlos Giovani Oliveira
  • Mesquita, Bruna Mara Aparecida de Carvalho
  • Santos, Sergio Henrique Sousa
  • Aguiar-Oliveira, Elizama
  • Cota, Junio

Abstract

Integrated bioprocess strategies may facilitate ethanol production from both C6 and C5 fractions of lignocellulosic feedstocks. We propose a new process concept, SSICF, where sugarcane bagasse is hydrolyzed simultaneously with xylose isomerization and the co-fermentation of C6 and C5 sugars. A commercial cocktail was supplemented with our multi-enzymatic system composed of three recombinant enzymes immobilized in Feroxyhyte magnetic nanoparticles: β-glucosidase, β-xylosidase and xylose isomerase. SSICF was performed using non-GMO Saccharomyces at pH 6.0 and 35 °C for 72 h in a synthetic medium containing cellobiose and xylose, and another medium containing pretreated sugarcane bagasse (PSB). The results of ethanol global yields in SSICF were 77.67% and 73.24% for the synthetic medium and PSB, respectively. In a nutshell, this is the first report of a successful proof-of-concept of SSICF with four rounds of enzyme recycling and a non-GMO yeast, an innovative process with high potential for industrial use.

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  • Guilherme, Ederson Paulo Xavier & Zanphorlin, Leticia Maria & Sousa, Amanda Silva & Miyamoto, Renan Yuji & Bruziquesi, Carlos Giovani Oliveira & Mesquita, Bruna Mara Aparecida de Carvalho & Santos, Se, 2022. "Simultaneous saccharification isomerization and Co-fermentation – SSICF: A new process concept for second-generation ethanol biorefineries combining immobilized recombinant enzymes and non-GMO Sacchar," Renewable Energy, Elsevier, vol. 182(C), pages 274-284.
    • Handle: RePEc:eee:renene:v:182:y:2022:i:c:p:274-284
    DOI: 10.1016/j.renene.2021.10.023
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    1. Cao, Xiyue & Xu, Hui & Li, Fosheng & Zou, Yijun & Ran, Yulu & Ma, Xiaorui & Cao, Yu & Xu, Qingrui & Qiao, Dairong & Cao, Yi, 2021. "One-step direct transesterification of wet yeast for biodiesel production catalyzed by magnetic nanoparticle-immobilized lipase," Renewable Energy, Elsevier, vol. 171(C), pages 11-21.
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    1. Kavitha, S. & Gajendran, T. & Saranya, K. & Selvakumar, P. & Manivasagan, V. & Jeevitha, S., 2022. "An insight - A statistical investigation of consolidated bioprocessing of Allium ascalonicum leaves to ethanol using Hangateiclostridium thermocellum KSMK1203 and synthetic consortium," Renewable Energy, Elsevier, vol. 187(C), pages 403-416.

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