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Megahertz pulse trains enable multi-hit serial femtosecond crystallography experiments at X-ray free electron lasers

Author

Listed:
  • Susannah Holmes

    (La Trobe University
    La Trobe University)

  • Henry J. Kirkwood

    (European XFEL)

  • Richard Bean

    (European XFEL)

  • Klaus Giewekemeyer

    (European XFEL)

  • Andrew V. Martin

    (RMIT University)

  • Marjan Hadian-Jazi

    (La Trobe University
    European XFEL
    Australian Nuclear Science and Technology Organisation (ANSTO))

  • Max O. Wiedorn

    (Deutsches Elektronen-Synchrotron DESY)

  • Dominik Oberthür

    (Deutsches Elektronen-Synchrotron DESY)

  • Hugh Marman

    (La Trobe University
    La Trobe University)

  • Luigi Adriano

    (Deutsches Elektronen-Synchrotron DESY)

  • Nasser Al-Qudami

    (European XFEL)

  • Saša Bajt

    (Deutsches Elektronen-Synchrotron DESY
    The Hamburg Centre for Ultrafast Imaging)

  • Imrich Barák

    (Institute of Molecular Biology, SAS)

  • Sadia Bari

    (Deutsches Elektronen-Synchrotron DESY)

  • Johan Bielecki

    (European XFEL)

  • Sandor Brockhauser

    (European XFEL)

  • Mathew A. Coleman

    (Lawrence Livermore National Laboratory)

  • Francisco Cruz-Mazo

    (Universidad de Sevilla
    Princeton University)

  • Cyril Danilevski

    (European XFEL)

  • Katerina Dörner

    (European XFEL)

  • Alfonso M. Gañán-Calvo

    (Universidad de Sevilla)

  • Rita Graceffa

    (European XFEL)

  • Hans Fanghor

    (European XFEL
    Max-Planck Institute for the Structure and Dynamics of Matter
    University of Southampton)

  • Michael Heymann

    (University of Stuttgart)

  • Matthias Frank

    (Lawrence Livermore National Laboratory)

  • Alexander Kaukher

    (European XFEL)

  • Yoonhee Kim

    (European XFEL)

  • Bostjan Kobe

    (University of Queensland)

  • Juraj Knoška

    (Deutsches Elektronen-Synchrotron DESY
    Universität Hamburg)

  • Torsten Laurus

    (Deutsches Elektronen-Synchrotron DESY)

  • Romain Letrun

    (European XFEL)

  • Luis Maia

    (European XFEL)

  • Marc Messerschmidt

    (Arizona State University)

  • Markus Metz

    (Deutsches Elektronen-Synchrotron DESY)

  • Thomas Michelat

    (European XFEL)

  • Grant Mills

    (European XFEL)

  • Serguei Molodtsov

    (European XFEL
    TU Bergakademie Freiberg
    ITMO University)

  • Diana C. F. Monteiro

    (The Hamburg Centre for Ultrafast Imaging
    Hauptman-Woodward Medical Research Institute)

  • Andrew J. Morgan

    (Deutsches Elektronen-Synchrotron DESY
    University of Melbourne)

  • Astrid Münnich

    (European XFEL)

  • Gisel E. Peña Murillo

    (Deutsches Elektronen-Synchrotron DESY)

  • Gianpietro Previtali

    (European XFEL)

  • Adam Round

    (European XFEL)

  • Tokushi Sato

    (European XFEL
    Deutsches Elektronen-Synchrotron DESY)

  • Robin Schubert

    (European XFEL)

  • Joachim Schulz

    (European XFEL)

  • Megan Shelby

    (Lawrence Livermore National Laboratory)

  • Carolin Seuring

    (Deutsches Elektronen-Synchrotron DESY
    The Hamburg Centre for Ultrafast Imaging)

  • Jonas A. Sellberg

    (AlbaNova University Center, KTH Royal Institute of Technology)

  • Marcin Sikorski

    (European XFEL)

  • Alessandro Silenzi

    (European XFEL)

  • Stephan Stern

    (European XFEL)

  • Jola Sztuk-Dambietz

    (European XFEL)

  • Janusz Szuba

    (European XFEL)

  • Martin Trebbin

    (TU Bergakademie Freiberg
    State University of New York at Buffalo)

  • Patrick Vagovic

    (European XFEL)

  • Thomas Ve

    (Griffith University)

  • Britta Weinhausen

    (European XFEL)

  • Krzysztof Wrona

    (European XFEL)

  • Paul Lourdu Xavier

    (European XFEL
    Deutsches Elektronen-Synchrotron DESY
    Max-Planck Institute for the Structure and Dynamics of Matter)

  • Chen Xu

    (European XFEL)

  • Oleksandr Yefanov

    (Deutsches Elektronen-Synchrotron DESY)

  • Keith A. Nugent

    (Australian National University)

  • Henry N. Chapman

    (Deutsches Elektronen-Synchrotron DESY
    The Hamburg Centre for Ultrafast Imaging
    Universität Hamburg)

  • Adrian P. Mancuso

    (European XFEL
    La Trobe University)

  • Anton Barty

    (Deutsches Elektronen-Synchrotron DESY)

  • Brian Abbey

    (La Trobe University
    La Trobe University)

  • Connie Darmanin

    (La Trobe University
    La Trobe University)

Abstract

The European X-ray Free Electron Laser (XFEL) and Linac Coherent Light Source (LCLS) II are extremely intense sources of X-rays capable of generating Serial Femtosecond Crystallography (SFX) data at megahertz (MHz) repetition rates. Previous work has shown that it is possible to use consecutive X-ray pulses to collect diffraction patterns from individual crystals. Here, we exploit the MHz pulse structure of the European XFEL to obtain two complete datasets from the same lysozyme crystal, first hit and the second hit, before it exits the beam. The two datasets, separated by

Suggested Citation

  • Susannah Holmes & Henry J. Kirkwood & Richard Bean & Klaus Giewekemeyer & Andrew V. Martin & Marjan Hadian-Jazi & Max O. Wiedorn & Dominik Oberthür & Hugh Marman & Luigi Adriano & Nasser Al-Qudami & S, 2022. "Megahertz pulse trains enable multi-hit serial femtosecond crystallography experiments at X-ray free electron lasers," Nature Communications, Nature, vol. 13(1), pages 1-13, December.
    • Handle: RePEc:nat:natcom:v:13:y:2022:i:1:d:10.1038_s41467-022-32434-6
    DOI: 10.1038/s41467-022-32434-6
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    References listed on IDEAS

    as
    1. Henry N. Chapman & Petra Fromme & Anton Barty & Thomas A. White & Richard A. Kirian & Andrew Aquila & Mark S. Hunter & Joachim Schulz & Daniel P. DePonte & Uwe Weierstall & R. Bruce Doak & Filipe R. N, 2011. "Femtosecond X-ray protein nanocrystallography," Nature, Nature, vol. 470(7332), pages 73-77, February.
    2. Karol Nass & Alexander Gorel & Malik M. Abdullah & Andrew V. Martin & Marco Kloos & Agostino Marinelli & Andrew Aquila & Thomas R. M. Barends & Franz-Josef Decker & R. Bruce Doak & Lutz Foucar & Elisa, 2020. "Structural dynamics in proteins induced by and probed with X-ray free-electron laser pulses," Nature Communications, Nature, vol. 11(1), pages 1-9, December.
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    Full references (including those not matched with items on IDEAS)

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