IDEAS home Printed from https://ideas.repec.org/a/nat/natcom/v11y2020i1d10.1038_s41467-020-18156-7.html
   My bibliography  Save this article

Segmented flow generator for serial crystallography at the European X-ray free electron laser

Author

Listed:
  • Austin Echelmeier

    (Arizona State University
    Arizona State University)

  • Jorvani Cruz Villarreal

    (Arizona State University
    Arizona State University)

  • Marc Messerschmidt

    (Arizona State University
    Arizona State University
    European XFEL)

  • Daihyun Kim

    (Arizona State University
    Arizona State University)

  • Jesse D. Coe

    (Arizona State University
    Arizona State University)

  • Darren Thifault

    (Arizona State University
    Arizona State University)

  • Sabine Botha

    (Arizona State University
    Arizona State University)

  • Ana Egatz-Gomez

    (Arizona State University
    Arizona State University)

  • Sahir Gandhi

    (Arizona State University
    Arizona State University)

  • Gerrit Brehm

    (University of Göttingen)

  • Chelsie E. Conrad

    (Arizona State University
    Arizona State University)

  • Debra T. Hansen

    (Arizona State University)

  • Caleb Madsen

    (Arizona State University
    Arizona State University)

  • Saša Bajt

    (Deutsches Elektronen-Synchrotron
    Universität Hamburg)

  • J. Domingo Meza-Aguilar

    (European XFEL)

  • Dominik Oberthür

    (Deutsches Elektronen-Synchrotron)

  • Max O. Wiedorn

    (Universität Hamburg
    Deutsches Elektronen-Synchrotron)

  • Holger Fleckenstein

    (Deutsches Elektronen-Synchrotron)

  • Derek Mendez

    (Arizona State University
    Arizona State University)

  • Juraj Knoška

    (Deutsches Elektronen-Synchrotron
    Universität Hamburg)

  • Jose M. Martin-Garcia

    (Arizona State University
    Arizona State University)

  • Hao Hu

    (Arizona State University
    Arizona State University)

  • Stella Lisova

    (Arizona State University
    Arizona State University)

  • Aschkan Allahgholi

    (Deutsches Elektronen-Synchrotron)

  • Yaroslav Gevorkov

    (Deutsches Elektronen-Synchrotron
    Hamburg University of Technology, Vision Systems E-2)

  • Kartik Ayyer

    (Deutsches Elektronen-Synchrotron)

  • Steve Aplin

    (Deutsches Elektronen-Synchrotron)

  • Helen Mary Ginn

    (University of Oxford
    Diamond Light Source Ltd)

  • Heinz Graafsma

    (Deutsches Elektronen-Synchrotron)

  • Andrew J. Morgan

    (Deutsches Elektronen-Synchrotron)

  • Dominic Greiffenberg

    (Paul Scherrer Institute)

  • Alexander Klujev

    (Deutsches Elektronen-Synchrotron)

  • Torsten Laurus

    (Deutsches Elektronen-Synchrotron)

  • Jennifer Poehlsen

    (Deutsches Elektronen-Synchrotron)

  • Ulrich Trunk

    (Deutsches Elektronen-Synchrotron)

  • Davide Mezza

    (Paul Scherrer Institute)

  • Bernd Schmidt

    (Paul Scherrer Institute)

  • Manuela Kuhn

    (Deutsches Elektronen-Synchrotron)

  • Raimund Fromme

    (Arizona State University
    Arizona State University)

  • Jolanta Sztuk-Dambietz

    (European XFEL)

  • Natascha Raab

    (European XFEL)

  • Steffen Hauf

    (European XFEL)

  • Alessandro Silenzi

    (European XFEL)

  • Thomas Michelat

    (European XFEL)

  • Chen Xu

    (European XFEL)

  • Cyril Danilevski

    (European XFEL)

  • Andrea Parenti

    (European XFEL)

  • Leonce Mekinda

    (European XFEL)

  • Britta Weinhausen

    (European XFEL)

  • Grant Mills

    (European XFEL)

  • Patrik Vagovic

    (European XFEL)

  • Yoonhee Kim

    (European XFEL)

  • Henry Kirkwood

    (European XFEL)

  • Richard Bean

    (European XFEL)

  • Johan Bielecki

    (European XFEL)

  • Stephan Stern

    (European XFEL
    Deutsches Elektronen-Synchrotron)

  • Klaus Giewekemeyer

    (European XFEL)

  • Adam R. Round

    (European XFEL
    Keele University)

  • Joachim Schulz

    (European XFEL)

  • Katerina Dörner

    (European XFEL)

  • Thomas D. Grant

    (SUNY University at Buffalo)

  • Valerio Mariani

    (Deutsches Elektronen-Synchrotron)

  • Anton Barty

    (Deutsches Elektronen-Synchrotron)

  • Adrian P. Mancuso

    (European XFEL
    La Trobe University)

  • Uwe Weierstall

    (Arizona State University
    Arizona State University)

  • John C. H. Spence

    (Arizona State University
    Arizona State University)

  • Henry N. Chapman

    (Universität Hamburg
    Deutsches Elektronen-Synchrotron
    Universität Hamburg)

  • Nadia Zatsepin

    (Arizona State University
    Arizona State University
    La Trobe University)

  • Petra Fromme

    (Arizona State University
    Arizona State University)

  • Richard A. Kirian

    (Arizona State University
    Arizona State University)

  • Alexandra Ros

    (Arizona State University
    Arizona State University)

Abstract

Serial femtosecond crystallography (SFX) with X-ray free electron lasers (XFELs) allows structure determination of membrane proteins and time-resolved crystallography. Common liquid sample delivery continuously jets the protein crystal suspension into the path of the XFEL, wasting a vast amount of sample due to the pulsed nature of all current XFEL sources. The European XFEL (EuXFEL) delivers femtosecond (fs) X-ray pulses in trains spaced 100 ms apart whereas pulses within trains are currently separated by 889 ns. Therefore, continuous sample delivery via fast jets wastes >99% of sample. Here, we introduce a microfluidic device delivering crystal laden droplets segmented with an immiscible oil reducing sample waste and demonstrate droplet injection at the EuXFEL compatible with high pressure liquid delivery of an SFX experiment. While achieving ~60% reduction in sample waste, we determine the structure of the enzyme 3-deoxy-D-manno-octulosonate-8-phosphate synthase from microcrystals delivered in droplets revealing distinct structural features not previously reported.

Suggested Citation

  • Austin Echelmeier & Jorvani Cruz Villarreal & Marc Messerschmidt & Daihyun Kim & Jesse D. Coe & Darren Thifault & Sabine Botha & Ana Egatz-Gomez & Sahir Gandhi & Gerrit Brehm & Chelsie E. Conrad & Deb, 2020. "Segmented flow generator for serial crystallography at the European X-ray free electron laser," Nature Communications, Nature, vol. 11(1), pages 1-10, December.
    • Handle: RePEc:nat:natcom:v:11:y:2020:i:1:d:10.1038_s41467-020-18156-7
    DOI: 10.1038/s41467-020-18156-7
    as

    Download full text from publisher

    File URL: https://www.nature.com/articles/s41467-020-18156-7
    File Function: Abstract
    Download Restriction: no
    File URL: https://libkey.io/10.1038/s41467-020-18156-7?utm_source=ideas
    LibKey link: if access is restricted and if your library uses this service, LibKey will redirect you to where you can use your library subscription to access this item
    ---><---

    Citations

    Citations are extracted by the CitEc Project, subscribe to its RSS feed for this item.
    as


    Cited by:

    1. Susannah Holmes & Henry J. Kirkwood & Richard Bean & Klaus Giewekemeyer & Andrew V. Martin & Marjan Hadian-Jazi & Max O. Wiedorn & Dominik Oberthür & Hugh Marman & Luigi Adriano & Nasser Al-Qudami & S, 2022. "Megahertz pulse trains enable multi-hit serial femtosecond crystallography experiments at X-ray free electron lasers," Nature Communications, Nature, vol. 13(1), pages 1-13, December.

    More about this item

    Statistics

    Access and download statistics

    Corrections

    All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:nat:natcom:v:11:y:2020:i:1:d:10.1038_s41467-020-18156-7. See general information about how to correct material in RePEc.

    If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.

    We have no bibliographic references for this item. You can help adding them by using this form .

    If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.

    For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: Sonal Shukla or Springer Nature Abstracting and Indexing (email available below). General contact details of provider: http://www.nature.com .

    Please note that corrections may take a couple of weeks to filter through the various RePEc services.

    IDEAS is a RePEc service. RePEc uses bibliographic data supplied by the respective publishers.