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Femtosecond X-ray protein nanocrystallography

Author

Listed:
  • Henry N. Chapman

    (Center for Free-Electron Laser Science, DESY, Notkestrasse 85, 22607 Hamburg, Germany
    University of Hamburg, Luruper Chaussee 149, 22761 Hamburg, Germany)

  • Petra Fromme

    (Arizona State University)

  • Anton Barty

    (Center for Free-Electron Laser Science, DESY, Notkestrasse 85, 22607 Hamburg, Germany)

  • Thomas A. White

    (Center for Free-Electron Laser Science, DESY, Notkestrasse 85, 22607 Hamburg, Germany)

  • Richard A. Kirian

    (Arizona State University)

  • Andrew Aquila

    (Center for Free-Electron Laser Science, DESY, Notkestrasse 85, 22607 Hamburg, Germany)

  • Mark S. Hunter

    (Arizona State University)

  • Joachim Schulz

    (Center for Free-Electron Laser Science, DESY, Notkestrasse 85, 22607 Hamburg, Germany)

  • Daniel P. DePonte

    (Center for Free-Electron Laser Science, DESY, Notkestrasse 85, 22607 Hamburg, Germany)

  • Uwe Weierstall

    (Arizona State University)

  • R. Bruce Doak

    (Arizona State University)

  • Filipe R. N. C. Maia

    (Laboratory of Molecular Biophysics, Uppsala University, Husargatan 3 (Box 596), SE-751 24 Uppsala, Sweden)

  • Andrew V. Martin

    (Center for Free-Electron Laser Science, DESY, Notkestrasse 85, 22607 Hamburg, Germany)

  • Ilme Schlichting

    (Max Planck Advanced Study Group, Center for Free-Electron Laser Science, Notkestrasse 85, 22607 Hamburg, Germany
    Max-Planck-Institut für Medizinische Forschung, Jahnstrasse 29, 69120 Heidelberg, Germany)

  • Lukas Lomb

    (Max-Planck-Institut für Medizinische Forschung, Jahnstrasse 29, 69120 Heidelberg, Germany)

  • Nicola Coppola

    (Center for Free-Electron Laser Science, DESY, Notkestrasse 85, 22607 Hamburg, Germany
    Present address: European XFEL GmbH, Notkestrasse 85, 22607 Hamburg, Germany)

  • Robert L. Shoeman

    (Max-Planck-Institut für Medizinische Forschung, Jahnstrasse 29, 69120 Heidelberg, Germany)

  • Sascha W. Epp

    (Max Planck Advanced Study Group, Center for Free-Electron Laser Science, Notkestrasse 85, 22607 Hamburg, Germany
    Max-Planck-Institut für Kernphysik, Saupfercheckweg 1, 69117 Heidelberg, Germany)

  • Robert Hartmann

    (PNSensor GmbH, Otto-Hahn-Ring 6, 81739 München, Germany)

  • Daniel Rolles

    (Max Planck Advanced Study Group, Center for Free-Electron Laser Science, Notkestrasse 85, 22607 Hamburg, Germany
    Max-Planck-Institut für Medizinische Forschung, Jahnstrasse 29, 69120 Heidelberg, Germany)

  • Artem Rudenko

    (Max Planck Advanced Study Group, Center for Free-Electron Laser Science, Notkestrasse 85, 22607 Hamburg, Germany
    Max-Planck-Institut für Kernphysik, Saupfercheckweg 1, 69117 Heidelberg, Germany)

  • Lutz Foucar

    (Max Planck Advanced Study Group, Center for Free-Electron Laser Science, Notkestrasse 85, 22607 Hamburg, Germany
    Max-Planck-Institut für Medizinische Forschung, Jahnstrasse 29, 69120 Heidelberg, Germany)

  • Nils Kimmel

    (Max-Planck-Institut Halbleiterlabor, Otto-Hahn-Ring 6, 81739 München, Germany)

  • Georg Weidenspointner

    (Max-Planck-Institut Halbleiterlabor, Otto-Hahn-Ring 6, 81739 München, Germany
    Max-Planck-Institut für Extraterrestrische Physik, Giessenbachstrasse, 85741 Garching, Germany)

  • Peter Holl

    (PNSensor GmbH, Otto-Hahn-Ring 6, 81739 München, Germany)

  • Mengning Liang

    (Center for Free-Electron Laser Science, DESY, Notkestrasse 85, 22607 Hamburg, Germany)

  • Miriam Barthelmess

    (Photon Science, DESY, Notkestrasse 85, 22607 Hamburg, Germany)

  • Carl Caleman

    (Center for Free-Electron Laser Science, DESY, Notkestrasse 85, 22607 Hamburg, Germany)

  • Sébastien Boutet

    (LCLS, SLAC National Accelerator Laboratory, 2575 Sand Hill Road, Menlo Park, California 94025, USA)

  • Michael J. Bogan

    (PULSE Institute, SLAC National Accelerator Laboratory, 2575 Sand Hill Road, Menlo Park, California 94025, USA)

  • Jacek Krzywinski

    (LCLS, SLAC National Accelerator Laboratory, 2575 Sand Hill Road, Menlo Park, California 94025, USA)

  • Christoph Bostedt

    (LCLS, SLAC National Accelerator Laboratory, 2575 Sand Hill Road, Menlo Park, California 94025, USA)

  • Saša Bajt

    (Photon Science, DESY, Notkestrasse 85, 22607 Hamburg, Germany)

  • Lars Gumprecht

    (Center for Free-Electron Laser Science, DESY, Notkestrasse 85, 22607 Hamburg, Germany)

  • Benedikt Rudek

    (Max Planck Advanced Study Group, Center for Free-Electron Laser Science, Notkestrasse 85, 22607 Hamburg, Germany
    Max-Planck-Institut für Kernphysik, Saupfercheckweg 1, 69117 Heidelberg, Germany)

  • Benjamin Erk

    (Max Planck Advanced Study Group, Center for Free-Electron Laser Science, Notkestrasse 85, 22607 Hamburg, Germany
    Max-Planck-Institut für Kernphysik, Saupfercheckweg 1, 69117 Heidelberg, Germany)

  • Carlo Schmidt

    (Max Planck Advanced Study Group, Center for Free-Electron Laser Science, Notkestrasse 85, 22607 Hamburg, Germany
    Max-Planck-Institut für Kernphysik, Saupfercheckweg 1, 69117 Heidelberg, Germany)

  • André Hömke

    (Max Planck Advanced Study Group, Center for Free-Electron Laser Science, Notkestrasse 85, 22607 Hamburg, Germany
    Max-Planck-Institut für Kernphysik, Saupfercheckweg 1, 69117 Heidelberg, Germany)

  • Christian Reich

    (PNSensor GmbH, Otto-Hahn-Ring 6, 81739 München, Germany)

  • Daniel Pietschner

    (Max-Planck-Institut Halbleiterlabor, Otto-Hahn-Ring 6, 81739 München, Germany)

  • Lothar Strüder

    (Max Planck Advanced Study Group, Center for Free-Electron Laser Science, Notkestrasse 85, 22607 Hamburg, Germany
    Max-Planck-Institut Halbleiterlabor, Otto-Hahn-Ring 6, 81739 München, Germany)

  • Günter Hauser

    (Max-Planck-Institut Halbleiterlabor, Otto-Hahn-Ring 6, 81739 München, Germany)

  • Hubert Gorke

    (Forschungszentrum Jülich, Institut ZEL, 52425 Jülich, Germany)

  • Joachim Ullrich

    (Max Planck Advanced Study Group, Center for Free-Electron Laser Science, Notkestrasse 85, 22607 Hamburg, Germany
    Max-Planck-Institut für Kernphysik, Saupfercheckweg 1, 69117 Heidelberg, Germany)

  • Sven Herrmann

    (Max-Planck-Institut Halbleiterlabor, Otto-Hahn-Ring 6, 81739 München, Germany)

  • Gerhard Schaller

    (Max-Planck-Institut Halbleiterlabor, Otto-Hahn-Ring 6, 81739 München, Germany)

  • Florian Schopper

    (Max-Planck-Institut Halbleiterlabor, Otto-Hahn-Ring 6, 81739 München, Germany)

  • Heike Soltau

    (PNSensor GmbH, Otto-Hahn-Ring 6, 81739 München, Germany)

  • Kai-Uwe Kühnel

    (Max-Planck-Institut für Kernphysik, Saupfercheckweg 1, 69117 Heidelberg, Germany)

  • Marc Messerschmidt

    (LCLS, SLAC National Accelerator Laboratory, 2575 Sand Hill Road, Menlo Park, California 94025, USA)

  • John D. Bozek

    (LCLS, SLAC National Accelerator Laboratory, 2575 Sand Hill Road, Menlo Park, California 94025, USA)

  • Stefan P. Hau-Riege

    (Lawrence Livermore National Laboratory, 7000 East Avenue, Mail Stop L-211, Livermore, California 94551, USA)

  • Matthias Frank

    (Lawrence Livermore National Laboratory, 7000 East Avenue, Mail Stop L-211, Livermore, California 94551, USA)

  • Christina Y. Hampton

    (PULSE Institute, SLAC National Accelerator Laboratory, 2575 Sand Hill Road, Menlo Park, California 94025, USA)

  • Raymond G. Sierra

    (PULSE Institute, SLAC National Accelerator Laboratory, 2575 Sand Hill Road, Menlo Park, California 94025, USA)

  • Dmitri Starodub

    (PULSE Institute, SLAC National Accelerator Laboratory, 2575 Sand Hill Road, Menlo Park, California 94025, USA)

  • Garth J. Williams

    (LCLS, SLAC National Accelerator Laboratory, 2575 Sand Hill Road, Menlo Park, California 94025, USA)

  • Janos Hajdu

    (Laboratory of Molecular Biophysics, Uppsala University, Husargatan 3 (Box 596), SE-751 24 Uppsala, Sweden)

  • Nicusor Timneanu

    (Laboratory of Molecular Biophysics, Uppsala University, Husargatan 3 (Box 596), SE-751 24 Uppsala, Sweden)

  • M. Marvin Seibert

    (Laboratory of Molecular Biophysics, Uppsala University, Husargatan 3 (Box 596), SE-751 24 Uppsala, Sweden
    Present address LCLS, SLAC National Accelerator Laboratory, 2575 Sand Hill Road, Menlo Park, California 94025, USA)

  • Jakob Andreasson

    (Laboratory of Molecular Biophysics, Uppsala University, Husargatan 3 (Box 596), SE-751 24 Uppsala, Sweden)

  • Andrea Rocker

    (Laboratory of Molecular Biophysics, Uppsala University, Husargatan 3 (Box 596), SE-751 24 Uppsala, Sweden)

  • Olof Jönsson

    (Laboratory of Molecular Biophysics, Uppsala University, Husargatan 3 (Box 596), SE-751 24 Uppsala, Sweden)

  • Martin Svenda

    (Laboratory of Molecular Biophysics, Uppsala University, Husargatan 3 (Box 596), SE-751 24 Uppsala, Sweden)

  • Stephan Stern

    (Center for Free-Electron Laser Science, DESY, Notkestrasse 85, 22607 Hamburg, Germany)

  • Karol Nass

    (University of Hamburg, Luruper Chaussee 149, 22761 Hamburg, Germany)

  • Robert Andritschke

    (Max-Planck-Institut Halbleiterlabor, Otto-Hahn-Ring 6, 81739 München, Germany)

  • Claus-Dieter Schröter

    (Max-Planck-Institut für Kernphysik, Saupfercheckweg 1, 69117 Heidelberg, Germany)

  • Faton Krasniqi

    (Max Planck Advanced Study Group, Center for Free-Electron Laser Science, Notkestrasse 85, 22607 Hamburg, Germany
    Max-Planck-Institut für Medizinische Forschung, Jahnstrasse 29, 69120 Heidelberg, Germany)

  • Mario Bott

    (Max-Planck-Institut für Medizinische Forschung, Jahnstrasse 29, 69120 Heidelberg, Germany)

  • Kevin E. Schmidt

    (Arizona State University)

  • Xiaoyu Wang

    (Arizona State University)

  • Ingo Grotjohann

    (Arizona State University)

  • James M. Holton

    (Advanced Light Source, Lawrence Berkeley National Laboratory)

  • Thomas R. M. Barends

    (Max-Planck-Institut für Medizinische Forschung, Jahnstrasse 29, 69120 Heidelberg, Germany)

  • Richard Neutze

    (Biochemistry and Biophysics, University of Gothenburg, SE-405 30 Gothenburg, Sweden)

  • Stefano Marchesini

    (Advanced Light Source, Lawrence Berkeley National Laboratory)

  • Raimund Fromme

    (Arizona State University)

  • Sebastian Schorb

    (Institut für Optik und Atomare Physik, Technische Universität Berlin, Hardenbergstrasse 36, 10623 Berlin, Germany)

  • Daniela Rupp

    (Institut für Optik und Atomare Physik, Technische Universität Berlin, Hardenbergstrasse 36, 10623 Berlin, Germany)

  • Marcus Adolph

    (Institut für Optik und Atomare Physik, Technische Universität Berlin, Hardenbergstrasse 36, 10623 Berlin, Germany)

  • Tais Gorkhover

    (Institut für Optik und Atomare Physik, Technische Universität Berlin, Hardenbergstrasse 36, 10623 Berlin, Germany)

  • Inger Andersson

    (Swedish University of Agricultural Sciences, Uppsala Biomedical Centre, Box 590, S-751 24 Uppsala, Sweden)

  • Helmut Hirsemann

    (Photon Science, DESY, Notkestrasse 85, 22607 Hamburg, Germany)

  • Guillaume Potdevin

    (Photon Science, DESY, Notkestrasse 85, 22607 Hamburg, Germany)

  • Heinz Graafsma

    (Photon Science, DESY, Notkestrasse 85, 22607 Hamburg, Germany)

  • Björn Nilsson

    (Photon Science, DESY, Notkestrasse 85, 22607 Hamburg, Germany)

  • John C. H. Spence

    (Arizona State University)

Abstract

Biological imaging with the LCLS X-ray laser The start-up of the Linac Coherent Light Source (LCLS), the new femtosecond hard X-ray laser facility in Stanford, California, has brought high expectations of a new era for biological imaging. The intense, ultrashort X-ray pulses allow diffraction imaging of small structures before radiation damage occurs. Two papers in this issue of Nature present proof-of-concept experiments showing the LCLS in action. Chapman et al. tackle structure determination from nanocrystals of macromolecules that cannot be grown in large crystals. They obtain more than three million diffraction patterns from a stream of nanocrystals of the membrane protein photosystem I, and assemble a three-dimensional data set for this protein. Seibert et al. obtain images of a non-crystalline biological sample, mimivirus, by injecting a beam of cooled mimivirus particles into the X-ray beam.

Suggested Citation

  • Henry N. Chapman & Petra Fromme & Anton Barty & Thomas A. White & Richard A. Kirian & Andrew Aquila & Mark S. Hunter & Joachim Schulz & Daniel P. DePonte & Uwe Weierstall & R. Bruce Doak & Filipe R. N, 2011. "Femtosecond X-ray protein nanocrystallography," Nature, Nature, vol. 470(7332), pages 73-77, February.
    • Handle: RePEc:nat:nature:v:470:y:2011:i:7332:d:10.1038_nature09750
    DOI: 10.1038/nature09750
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    2. Maximilian Wranik & Michal W. Kepa & Emma V. Beale & Daniel James & Quentin Bertrand & Tobias Weinert & Antonia Furrer & Hannah Glover & Dardan Gashi & Melissa Carrillo & Yasushi Kondo & Robin T. Stip, 2023. "A multi-reservoir extruder for time-resolved serial protein crystallography and compound screening at X-ray free-electron lasers," Nature Communications, Nature, vol. 14(1), pages 1-12, December.
    3. Guillaume Tetreau & Michael R. Sawaya & Elke Zitter & Elena A. Andreeva & Anne-Sophie Banneville & Natalie A. Schibrowsky & Nicolas Coquelle & Aaron S. Brewster & Marie Luise Grünbein & Gabriela Nass , 2022. "De novo determination of mosquitocidal Cry11Aa and Cry11Ba structures from naturally-occurring nanocrystals," Nature Communications, Nature, vol. 13(1), pages 1-18, December.
    4. Basudev Maity & Mitsuo Shoji & Fangjia Luo & Takanori Nakane & Satoshi Abe & Shigeki Owada & Jungmin Kang & Kensuke Tono & Rie Tanaka & Thuc Toan Pham & Mariko Kojima & Yuki Hishikawa & Junko Tanaka &, 2024. "Real-time observation of a metal complex-driven reaction intermediate using a porous protein crystal and serial femtosecond crystallography," Nature Communications, Nature, vol. 15(1), pages 1-12, December.
    5. Mario Reiser & Anita Girelli & Anastasia Ragulskaya & Sudipta Das & Sharon Berkowicz & Maddalena Bin & Marjorie Ladd-Parada & Mariia Filianina & Hanna-Friederike Poggemann & Nafisa Begam & Mohammad Sa, 2022. "Resolving molecular diffusion and aggregation of antibody proteins with megahertz X-ray free-electron laser pulses," Nature Communications, Nature, vol. 13(1), pages 1-10, December.
    6. Lin Zhang & Beibei Sun & Fei Shu & Ying Huang, 2022. "Comparing paper level classifications across different methods and systems: an investigation of Nature publications," Scientometrics, Springer;Akadémiai Kiadó, vol. 127(12), pages 7633-7651, December.
    7. M. Wilamowski & D. A. Sherrell & Y. Kim & A. Lavens & R. W. Henning & K. Lazarski & A. Shigemoto & M. Endres & N. Maltseva & G. Babnigg & S. C. Burdette & V. Srajer & A. Joachimiak, 2022. "Time-resolved β-lactam cleavage by L1 metallo-β-lactamase," Nature Communications, Nature, vol. 13(1), pages 1-12, December.
    8. Da Liu & Yichu Zheng & Xin Yuan Sui & Xue Feng Wu & Can Zou & Yu Peng & Xinyi Liu & Miaoyu Lin & Zhanpeng Wei & Hang Zhou & Ye-Feng Yao & Sheng Dai & Haiyang Yuan & Hua Gui Yang & Shuang Yang & Yu Hou, 2024. "Universal growth of perovskite thin monocrystals from high solute flux for sensitive self-driven X-ray detection," Nature Communications, Nature, vol. 15(1), pages 1-10, December.
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    10. Kevin M. Dalton & Jack B. Greisman & Doeke R. Hekstra, 2022. "A unifying Bayesian framework for merging X-ray diffraction data," Nature Communications, Nature, vol. 13(1), pages 1-13, December.
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