IDEAS home Printed from https://ideas.repec.org/a/nat/natcom/v5y2014i1d10.1038_ncomms4309.html
   My bibliography  Save this article

Lipidic cubic phase injector facilitates membrane protein serial femtosecond crystallography

Author

Listed:
  • Uwe Weierstall

    (Arizona State University)

  • Daniel James

    (Arizona State University)

  • Chong Wang

    (The Scripps Research Institute)

  • Thomas A. White

    (Center for Free-Electron Laser Science, DESY, Notkestrasse 85)

  • Dingjie Wang

    (Arizona State University)

  • Wei Liu

    (The Scripps Research Institute)

  • John C. H. Spence

    (Arizona State University)

  • R. Bruce Doak

    (Arizona State University)

  • Garrett Nelson

    (Arizona State University)

  • Petra Fromme

    (Arizona State University)

  • Raimund Fromme

    (Arizona State University)

  • Ingo Grotjohann

    (Arizona State University)

  • Christopher Kupitz

    (Arizona State University)

  • Nadia A. Zatsepin

    (Arizona State University)

  • Haiguang Liu

    (Arizona State University)

  • Shibom Basu

    (Arizona State University)

  • Daniel Wacker

    (The Scripps Research Institute)

  • Gye Won Han

    (The Scripps Research Institute)

  • Vsevolod Katritch

    (The Scripps Research Institute)

  • Sébastien Boutet

    (SLAC National Accelerator Laboratory, 2575 Sand Hill Road)

  • Marc Messerschmidt

    (SLAC National Accelerator Laboratory, 2575 Sand Hill Road)

  • Garth J. Williams

    (SLAC National Accelerator Laboratory, 2575 Sand Hill Road)

  • Jason E. Koglin

    (SLAC National Accelerator Laboratory, 2575 Sand Hill Road)

  • M. Marvin Seibert

    (SLAC National Accelerator Laboratory, 2575 Sand Hill Road
    Laboratory of Molecular Biophysics, Uppsala University, Husargatan 3 (Box 596))

  • Markus Klinker

    (Center for Free-Electron Laser Science, DESY, Notkestrasse 85
    Universidad Autónoma de Madrid, Ciudad Universitaria de Cantoblanco)

  • Cornelius Gati

    (Center for Free-Electron Laser Science, DESY, Notkestrasse 85)

  • Robert L. Shoeman

    (Max-Planck-Institut für medizinische Forschung, Jahnstrasse 29)

  • Anton Barty

    (Center for Free-Electron Laser Science, DESY, Notkestrasse 85)

  • Henry N. Chapman

    (Center for Free-Electron Laser Science, DESY, Notkestrasse 85
    University of Hamburg
    Center for Ultrafast Imaging)

  • Richard A. Kirian

    (Arizona State University
    Center for Free-Electron Laser Science, DESY, Notkestrasse 85)

  • Kenneth R. Beyerlein

    (Center for Free-Electron Laser Science, DESY, Notkestrasse 85)

  • Raymond C. Stevens

    (The Scripps Research Institute)

  • Dianfan Li

    (School of Medicine and School of Biochemistry and Immunology, Trinity College)

  • Syed T. A. Shah

    (School of Medicine and School of Biochemistry and Immunology, Trinity College)

  • Nicole Howe

    (School of Medicine and School of Biochemistry and Immunology, Trinity College)

  • Martin Caffrey

    (School of Medicine and School of Biochemistry and Immunology, Trinity College)

  • Vadim Cherezov

    (The Scripps Research Institute)

Abstract

Lipidic cubic phase (LCP) crystallization has proven successful for high-resolution structure determination of challenging membrane proteins. Here we present a technique for extruding gel-like LCP with embedded membrane protein microcrystals, providing a continuously renewed source of material for serial femtosecond crystallography. Data collected from sub-10-μm-sized crystals produced with less than 0.5 mg of purified protein yield structural insights regarding cyclopamine binding to the Smoothened receptor.

Suggested Citation

  • Uwe Weierstall & Daniel James & Chong Wang & Thomas A. White & Dingjie Wang & Wei Liu & John C. H. Spence & R. Bruce Doak & Garrett Nelson & Petra Fromme & Raimund Fromme & Ingo Grotjohann & Christoph, 2014. "Lipidic cubic phase injector facilitates membrane protein serial femtosecond crystallography," Nature Communications, Nature, vol. 5(1), pages 1-6, May.
    • Handle: RePEc:nat:natcom:v:5:y:2014:i:1:d:10.1038_ncomms4309
    DOI: 10.1038/ncomms4309
    as

    Download full text from publisher

    File URL: https://www.nature.com/articles/ncomms4309
    File Function: Abstract
    Download Restriction: no
    File URL: https://libkey.io/10.1038/ncomms4309?utm_source=ideas
    LibKey link: if access is restricted and if your library uses this service, LibKey will redirect you to where you can use your library subscription to access this item
    ---><---

    Citations

    Citations are extracted by the CitEc Project, subscribe to its RSS feed for this item.
    as


    Cited by:

    1. Susannah Holmes & Henry J. Kirkwood & Richard Bean & Klaus Giewekemeyer & Andrew V. Martin & Marjan Hadian-Jazi & Max O. Wiedorn & Dominik Oberthür & Hugh Marman & Luigi Adriano & Nasser Al-Qudami & S, 2022. "Megahertz pulse trains enable multi-hit serial femtosecond crystallography experiments at X-ray free electron lasers," Nature Communications, Nature, vol. 13(1), pages 1-13, December.
    2. Michael W. Martynowycz & Anna Shiriaeva & Max T. B. Clabbers & William J. Nicolas & Sara J. Weaver & Johan Hattne & Tamir Gonen, 2023. "A robust approach for MicroED sample preparation of lipidic cubic phase embedded membrane protein crystals," Nature Communications, Nature, vol. 14(1), pages 1-15, December.
    3. Kaihua Zhang & Hao Wu & Nicholas Hoppe & Aashish Manglik & Yifan Cheng, 2022. "Fusion protein strategies for cryo-EM study of G protein-coupled receptors," Nature Communications, Nature, vol. 13(1), pages 1-11, December.
    4. Maximilian Wranik & Michal W. Kepa & Emma V. Beale & Daniel James & Quentin Bertrand & Tobias Weinert & Antonia Furrer & Hannah Glover & Dardan Gashi & Melissa Carrillo & Yasushi Kondo & Robin T. Stip, 2023. "A multi-reservoir extruder for time-resolved serial protein crystallography and compound screening at X-ray free-electron lasers," Nature Communications, Nature, vol. 14(1), pages 1-12, December.
    5. Maximilian Wranik & Tobias Weinert & Chavdar Slavov & Tiziana Masini & Antonia Furrer & Natacha Gaillard & Dario Gioia & Marco Ferrarotti & Daniel James & Hannah Glover & Melissa Carrillo & Demet Keki, 2023. "Watching the release of a photopharmacological drug from tubulin using time-resolved serial crystallography," Nature Communications, Nature, vol. 14(1), pages 1-12, December.
    6. Elizaveta Lyapina & Egor Marin & Anastasiia Gusach & Philipp Orekhov & Andrey Gerasimov & Aleksandra Luginina & Daniil Vakhrameev & Margarita Ergasheva & Margarita Kovaleva & Georgii Khusainov & Polin, 2022. "Structural basis for receptor selectivity and inverse agonism in S1P5 receptors," Nature Communications, Nature, vol. 13(1), pages 1-14, December.

    More about this item

    Statistics

    Access and download statistics

    Corrections

    All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:nat:natcom:v:5:y:2014:i:1:d:10.1038_ncomms4309. See general information about how to correct material in RePEc.

    If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.

    We have no bibliographic references for this item. You can help adding them by using this form .

    If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.

    For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: Sonal Shukla or Springer Nature Abstracting and Indexing (email available below). General contact details of provider: http://www.nature.com .

    Please note that corrections may take a couple of weeks to filter through the various RePEc services.

    IDEAS is a RePEc service. RePEc uses bibliographic data supplied by the respective publishers.