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Resolving molecular diffusion and aggregation of antibody proteins with megahertz X-ray free-electron laser pulses

Author

Listed:
  • Mario Reiser

    (AlbaNova University Center, Stockholm University)

  • Anita Girelli

    (Institut für Angewandte Physik, Universität Tübingen)

  • Anastasia Ragulskaya

    (Institut für Angewandte Physik, Universität Tübingen)

  • Sudipta Das

    (AlbaNova University Center, Stockholm University)

  • Sharon Berkowicz

    (AlbaNova University Center, Stockholm University)

  • Maddalena Bin

    (AlbaNova University Center, Stockholm University)

  • Marjorie Ladd-Parada

    (AlbaNova University Center, Stockholm University)

  • Mariia Filianina

    (AlbaNova University Center, Stockholm University)

  • Hanna-Friederike Poggemann

    (AlbaNova University Center, Stockholm University
    Institut für Angewandte Physik, Universität Tübingen)

  • Nafisa Begam

    (Institut für Angewandte Physik, Universität Tübingen)

  • Mohammad Sayed Akhundzadeh

    (Universität Siegen)

  • Sonja Timmermann

    (Universität Siegen)

  • Lisa Randolph

    (Universität Siegen)

  • Yuriy Chushkin

    (ESRF - The European Synchrotron)

  • Tilo Seydel

    (Institut Laue-Langevin)

  • Ulrike Boesenberg

    (European X-Ray Free-Electron Laser Facility)

  • Jörg Hallmann

    (European X-Ray Free-Electron Laser Facility)

  • Johannes Möller

    (European X-Ray Free-Electron Laser Facility)

  • Angel Rodriguez-Fernandez

    (European X-Ray Free-Electron Laser Facility)

  • Robert Rosca

    (European X-Ray Free-Electron Laser Facility)

  • Robert Schaffer

    (European X-Ray Free-Electron Laser Facility)

  • Markus Scholz

    (European X-Ray Free-Electron Laser Facility)

  • Roman Shayduk

    (European X-Ray Free-Electron Laser Facility)

  • Alexey Zozulya

    (European X-Ray Free-Electron Laser Facility)

  • Anders Madsen

    (European X-Ray Free-Electron Laser Facility)

  • Frank Schreiber

    (Institut für Angewandte Physik, Universität Tübingen)

  • Fajun Zhang

    (Institut für Angewandte Physik, Universität Tübingen)

  • Fivos Perakis

    (AlbaNova University Center, Stockholm University)

  • Christian Gutt

    (Universität Siegen)

Abstract

X-ray free-electron lasers (XFELs) with megahertz repetition rate can provide novel insights into structural dynamics of biological macromolecule solutions. However, very high dose rates can lead to beam-induced dynamics and structural changes due to radiation damage. Here, we probe the dynamics of dense antibody protein (Ig-PEG) solutions using megahertz X-ray photon correlation spectroscopy (MHz-XPCS) at the European XFEL. By varying the total dose and dose rate, we identify a regime for measuring the motion of proteins in their first coordination shell, quantify XFEL-induced effects such as driven motion, and map out the extent of agglomeration dynamics. The results indicate that for average dose rates below 1.06 kGy μs−1 in a time window up to 10 μs, it is possible to capture the protein dynamics before the onset of beam induced aggregation. We refer to this approach as correlation before aggregation and demonstrate that MHz-XPCS bridges an important spatio-temporal gap in measurement techniques for biological samples.

Suggested Citation

  • Mario Reiser & Anita Girelli & Anastasia Ragulskaya & Sudipta Das & Sharon Berkowicz & Maddalena Bin & Marjorie Ladd-Parada & Mariia Filianina & Hanna-Friederike Poggemann & Nafisa Begam & Mohammad Sa, 2022. "Resolving molecular diffusion and aggregation of antibody proteins with megahertz X-ray free-electron laser pulses," Nature Communications, Nature, vol. 13(1), pages 1-10, December.
    • Handle: RePEc:nat:natcom:v:13:y:2022:i:1:d:10.1038_s41467-022-33154-7
    DOI: 10.1038/s41467-022-33154-7
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    References listed on IDEAS

    as
    1. Henry N. Chapman & Petra Fromme & Anton Barty & Thomas A. White & Richard A. Kirian & Andrew Aquila & Mark S. Hunter & Joachim Schulz & Daniel P. DePonte & Uwe Weierstall & R. Bruce Doak & Filipe R. N, 2011. "Femtosecond X-ray protein nanocrystallography," Nature, Nature, vol. 470(7332), pages 73-77, February.
    2. Richard Neutze & Remco Wouts & David van der Spoel & Edgar Weckert & Janos Hajdu, 2000. "Potential for biomolecular imaging with femtosecond X-ray pulses," Nature, Nature, vol. 406(6797), pages 752-757, August.
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    1. Nimmi Das Anthuparambil & Anita Girelli & Sonja Timmermann & Marvin Kowalski & Mohammad Sayed Akhundzadeh & Sebastian Retzbach & Maximilian D. Senft & Michelle Dargasz & Dennis Gutmüller & Anusha Hire, 2023. "Exploring non-equilibrium processes and spatio-temporal scaling laws in heated egg yolk using coherent X-rays," Nature Communications, Nature, vol. 14(1), pages 1-12, December.

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