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Conformational Dynamics of Response Regulator RegX3 from Mycobacterium tuberculosis

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Listed:
  • Ashfaq Ahmad
  • Yongfei Cai
  • Xingqiang Chen
  • Jianwei Shuai
  • Aidong Han

Abstract

Two-component signal transduction systems (TCS) are vital for adaptive responses to various environmental stresses in bacteria, fungi and even plants. A TCS typically comprises of a sensor histidine kinase (SK) with its cognate response regulator (RR), which often has two domains—N terminal receiver domain (RD) and C terminal effector domain (ED). The histidine kinase phosphorylates the RD to activate the ED by promoting dimerization. However, despite significant progress on structural studies, how RR transmits activation signal from RD to ED remains elusive. Here we analyzed active to inactive transition process of OmpR/PhoB family using an active conformation of RegX3 from Mycobacterium tuberculosis as a model system by computational approaches. An inactive state of RegX3 generated from 150 ns molecular dynamic simulation has rotameric conformations of Thr79 and Tyr98 that are generally conserved in inactive RRs. Arg81 in loop β4α4 acts synergistically with loop β1α1 to change its interaction partners during active to inactive transition, potentially leading to the N-terminal movement of RegX3 helix α1. Global conformational dynamics of RegX3 is mainly dependent on α4β5 region, in particular seven ‘hot-spot’ residues (Tyr98 to Ser104), adjacent to which several coevolved residues at dimeric interface, including Ile76-Asp96, Asp97-Arg111 and Glu24-Arg113 pairs, are critical for signal transduction. Taken together, our computational analyses suggest a molecular linkage between Asp phosphorylation, proximal loops and α4β5α5 dimeric interface during RR active to inactive state transition, which is not often evidently defined from static crystal structures.

Suggested Citation

  • Ashfaq Ahmad & Yongfei Cai & Xingqiang Chen & Jianwei Shuai & Aidong Han, 2015. "Conformational Dynamics of Response Regulator RegX3 from Mycobacterium tuberculosis," PLOS ONE, Public Library of Science, vol. 10(7), pages 1-18, July.
  • Handle: RePEc:plo:pone00:0133389
    DOI: 10.1371/journal.pone.0133389
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    References listed on IDEAS

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    1. Victoria A. Feher & John Cavanagh, 1999. "Millisecond-timescale motions contribute to the function of the bacterial response regulator protein Spo0F," Nature, Nature, vol. 400(6741), pages 289-293, July.
    2. Dorothee Kern & Brian F. Volkman & Peter Luginbühl & Michael J. Nohaile & Sydney Kustu & David E. Wemmer, 1999. "Structure of a transiently phosphorylated switch in bacterial signal transduction," Nature, Nature, vol. 402(6764), pages 894-898, December.
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