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Structure and distinct supramolecular organization of a PSII-ACPII dimer from a cryptophyte alga Chroomonas placoidea

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  • Zhiyuan Mao

    (Chinese Academy of Sciences
    University of Chinese Academy of Sciences)

  • Xingyue Li

    (Chinese Academy of Sciences
    University of Chinese Academy of Sciences)

  • Zhenhua Li

    (Chinese Academy of Sciences
    University of Chinese Academy of Sciences)

  • Liangliang Shen

    (Chinese Academy of Sciences
    Southern University of Science and Technology
    China National Botanical Garden)

  • Xiaoyi Li

    (Chinese Academy of Sciences
    China National Botanical Garden)

  • Yanyan Yang

    (Chinese Academy of Sciences
    China National Botanical Garden)

  • Wenda Wang

    (Chinese Academy of Sciences
    China National Botanical Garden
    National Center of Technology Innovation for Comprehensive Utilization of Saline-Alkali Land)

  • Tingyun Kuang

    (Chinese Academy of Sciences
    China National Botanical Garden
    National Center of Technology Innovation for Comprehensive Utilization of Saline-Alkali Land)

  • Jian-Ren Shen

    (Chinese Academy of Sciences
    China National Botanical Garden
    Okayama University)

  • Guangye Han

    (Chinese Academy of Sciences
    China National Botanical Garden
    National Center of Technology Innovation for Comprehensive Utilization of Saline-Alkali Land)

Abstract

Cryptophyte algae are an evolutionarily distinct and ecologically important group of photosynthetic unicellular eukaryotes. Photosystem II (PSII) of cryptophyte algae associates with alloxanthin chlorophyll a/c-binding proteins (ACPs) to act as the peripheral light-harvesting system, whose supramolecular organization is unknown. Here, we purify the PSII-ACPII supercomplex from a cryptophyte alga Chroomonas placoidea (C. placoidea), and analyze its structure at a resolution of 2.47 Å using cryo-electron microscopy. This structure reveals a dimeric organization of PSII-ACPII containing two PSII core monomers flanked by six symmetrically arranged ACPII subunits. The PSII core is conserved whereas the organization of ACPII subunits exhibits a distinct pattern, different from those observed so far in PSII of other algae and higher plants. Furthermore, we find a Chl a-binding antenna subunit, CCPII-S, which mediates interaction of ACPII with the PSII core. These results provide a structural basis for the assembly of antennas within the supercomplex and possible excitation energy transfer pathways in cryptophyte algal PSII, shedding light on the diversity of supramolecular organization of photosynthetic machinery.

Suggested Citation

  • Zhiyuan Mao & Xingyue Li & Zhenhua Li & Liangliang Shen & Xiaoyi Li & Yanyan Yang & Wenda Wang & Tingyun Kuang & Jian-Ren Shen & Guangye Han, 2024. "Structure and distinct supramolecular organization of a PSII-ACPII dimer from a cryptophyte alga Chroomonas placoidea," Nature Communications, Nature, vol. 15(1), pages 1-14, December.
  • Handle: RePEc:nat:natcom:v:15:y:2024:i:1:d:10.1038_s41467-024-48878-x
    DOI: 10.1038/s41467-024-48878-x
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    1. Long Si & Shumeng Zhang & Xiaodong Su & Mei Li, 2024. "Structural basis for the distinct core-antenna assembly of cryptophyte photosystem II," Nature Communications, Nature, vol. 15(1), pages 1-15, December.

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