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Structure of spinach photosystem II–LHCII supercomplex at 3.2 Å resolution

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  • Xuepeng Wei

    (National Laboratory of Biomacromolecules, CAS Center for Excellence in Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences
    University of Chinese Academy of Sciences)

  • Xiaodong Su

    (National Laboratory of Biomacromolecules, CAS Center for Excellence in Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences)

  • Peng Cao

    (National Laboratory of Biomacromolecules, CAS Center for Excellence in Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences)

  • Xiuying Liu

    (National Laboratory of Biomacromolecules, CAS Center for Excellence in Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences
    University of Chinese Academy of Sciences)

  • Wenrui Chang

    (National Laboratory of Biomacromolecules, CAS Center for Excellence in Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences)

  • Mei Li

    (National Laboratory of Biomacromolecules, CAS Center for Excellence in Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences)

  • Xinzheng Zhang

    (National Laboratory of Biomacromolecules, CAS Center for Excellence in Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences)

  • Zhenfeng Liu

    (National Laboratory of Biomacromolecules, CAS Center for Excellence in Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences)

Abstract

During photosynthesis, the plant photosystem II core complex receives excitation energy from the peripheral light-harvesting complex II (LHCII). The pathways along which excitation energy is transferred between them, and their assembly mechanisms, remain to be deciphered through high-resolution structural studies. Here we report the structure of a 1.1-megadalton spinach photosystem II–LHCII supercomplex solved at 3.2 Å resolution through single-particle cryo-electron microscopy. The structure reveals a homodimeric supramolecular system in which each monomer contains 25 protein subunits, 105 chlorophylls, 28 carotenoids and other cofactors. Three extrinsic subunits (PsbO, PsbP and PsbQ), which are essential for optimal oxygen-evolving activity of photosystem II, form a triangular crown that shields the Mn4CaO5-binding domains of CP43 and D1. One major trimeric and two minor monomeric LHCIIs associate with each core-complex monomer, and the antenna–core interactions are reinforced by three small intrinsic subunits (PsbW, PsbH and PsbZ). By analysing the closely connected interfacial chlorophylls, we have obtained detailed insights into the energy-transfer pathways between the antenna and core complexes.

Suggested Citation

  • Xuepeng Wei & Xiaodong Su & Peng Cao & Xiuying Liu & Wenrui Chang & Mei Li & Xinzheng Zhang & Zhenfeng Liu, 2016. "Structure of spinach photosystem II–LHCII supercomplex at 3.2 Å resolution," Nature, Nature, vol. 534(7605), pages 69-74, June.
    • Handle: RePEc:nat:nature:v:534:y:2016:i:7605:d:10.1038_nature18020
    DOI: 10.1038/nature18020
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    Cited by:

    1. Hou, Jingxiang & Liu, Xuezhi & Zhang, Jiarui & Wei, Zhenhua & Ma, Yingying & Wan, Heng & Liu, Jie & Cui, Bingjing & Zong, Yuzheng & Chen, Yiting & Liang, Kehao & Liu, Fulai, 2023. "Combined application of biochar and partial root-zone drying irrigation improves water relations and water use efficiency of cotton plants under salt stress," Agricultural Water Management, Elsevier, vol. 290(C).
    2. Zhiyuan Mao & Xingyue Li & Zhenhua Li & Liangliang Shen & Xiaoyi Li & Yanyan Yang & Wenda Wang & Tingyun Kuang & Jian-Ren Shen & Guangye Han, 2024. "Structure and distinct supramolecular organization of a PSII-ACPII dimer from a cryptophyte alga Chroomonas placoidea," Nature Communications, Nature, vol. 15(1), pages 1-14, December.
    3. Yu-Zhong Zhang & Kang Li & Bing-Yue Qin & Jian-Ping Guo & Quan-Bao Zhang & Dian-Li Zhao & Xiu-Lan Chen & Jun Gao & Lu-Ning Liu & Long-Sheng Zhao, 2024. "Structure of cryptophyte photosystem II–light-harvesting antennae supercomplex," Nature Communications, Nature, vol. 15(1), pages 1-12, December.
    4. Makiko Kosugi & Masato Kawasaki & Yutaka Shibata & Kojiro Hara & Shinichi Takaichi & Toshio Moriya & Naruhiko Adachi & Yasuhiro Kamei & Yasuhiro Kashino & Sakae Kudoh & Hiroyuki Koike & Toshiya Senda, 2023. "Uphill energy transfer mechanism for photosynthesis in an Antarctic alga," Nature Communications, Nature, vol. 14(1), pages 1-14, December.
    5. Songhao Zhao & Lili Shen & Xiaoyi Li & Qiushuang Tao & Zhenhua Li & Caizhe Xu & Cuicui Zhou & Yanyan Yang & Min Sang & Guangye Han & Long-Jiang Yu & Tingyun Kuang & Jian-Ren Shen & Wenda Wang, 2023. "Structural insights into photosystem II supercomplex and trimeric FCP antennae of a centric diatom Cyclotella meneghiniana," Nature Communications, Nature, vol. 14(1), pages 1-15, December.
    6. Ryo Nagao & Koji Kato & Minoru Kumazawa & Kentaro Ifuku & Makio Yokono & Takehiro Suzuki & Naoshi Dohmae & Fusamichi Akita & Seiji Akimoto & Naoyuki Miyazaki & Jian-Ren Shen, 2022. "Structural basis for different types of hetero-tetrameric light-harvesting complexes in a diatom PSII-FCPII supercomplex," Nature Communications, Nature, vol. 13(1), pages 1-12, December.

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