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Structural basis for different types of hetero-tetrameric light-harvesting complexes in a diatom PSII-FCPII supercomplex

Author

Listed:
  • Ryo Nagao

    (Okayama University)

  • Koji Kato

    (Okayama University)

  • Minoru Kumazawa

    (Kyoto University)

  • Kentaro Ifuku

    (Kyoto University)

  • Makio Yokono

    (Hokkaido University)

  • Takehiro Suzuki

    (Biomolecular Characterization Unit, RIKEN Center for Sustainable Resource Science)

  • Naoshi Dohmae

    (Biomolecular Characterization Unit, RIKEN Center for Sustainable Resource Science)

  • Fusamichi Akita

    (Okayama University)

  • Seiji Akimoto

    (Kobe University)

  • Naoyuki Miyazaki

    (University of Tsukuba)

  • Jian-Ren Shen

    (Okayama University)

Abstract

Fucoxanthin chlorophyll (Chl) a/c-binding proteins (FCPs) function as light harvesters in diatoms. The structure of a diatom photosystem II-FCPII (PSII-FCPII) supercomplex have been solved by cryo-electron microscopy (cryo-EM) previously; however, the FCPII subunits that constitute the FCPII tetramers and monomers are not identified individually due to their low resolutions. Here, we report a 2.5 Å resolution structure of the PSII-FCPII supercomplex using cryo-EM. Two types of tetrameric FCPs, S-tetramer, and M-tetramer, are identified as different types of hetero-tetrameric complexes. In addition, three FCP monomers, m1, m2, and m3, are assigned to different gene products of FCP. The present structure also identifies the positions of most Chls c and diadinoxanthins, which form a complicated pigment network. Excitation-energy transfer from FCPII to PSII is revealed by time-resolved fluorescence spectroscopy. These structural and spectroscopic findings provide insights into an assembly model of FCPII and its excitation-energy transfer and quenching processes.

Suggested Citation

  • Ryo Nagao & Koji Kato & Minoru Kumazawa & Kentaro Ifuku & Makio Yokono & Takehiro Suzuki & Naoshi Dohmae & Fusamichi Akita & Seiji Akimoto & Naoyuki Miyazaki & Jian-Ren Shen, 2022. "Structural basis for different types of hetero-tetrameric light-harvesting complexes in a diatom PSII-FCPII supercomplex," Nature Communications, Nature, vol. 13(1), pages 1-12, December.
    • Handle: RePEc:nat:natcom:v:13:y:2022:i:1:d:10.1038_s41467-022-29294-5
    DOI: 10.1038/s41467-022-29294-5
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    References listed on IDEAS

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    1. Xuepeng Wei & Xiaodong Su & Peng Cao & Xiuying Liu & Wenrui Chang & Mei Li & Xinzheng Zhang & Zhenfeng Liu, 2016. "Structure of spinach photosystem II–LHCII supercomplex at 3.2 Å resolution," Nature, Nature, vol. 534(7605), pages 69-74, June.
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    Cited by:

    1. Zhiyuan Mao & Xingyue Li & Zhenhua Li & Liangliang Shen & Xiaoyi Li & Yanyan Yang & Wenda Wang & Tingyun Kuang & Jian-Ren Shen & Guangye Han, 2024. "Structure and distinct supramolecular organization of a PSII-ACPII dimer from a cryptophyte alga Chroomonas placoidea," Nature Communications, Nature, vol. 15(1), pages 1-14, December.
    2. Yu-Zhong Zhang & Kang Li & Bing-Yue Qin & Jian-Ping Guo & Quan-Bao Zhang & Dian-Li Zhao & Xiu-Lan Chen & Jun Gao & Lu-Ning Liu & Long-Sheng Zhao, 2024. "Structure of cryptophyte photosystem II–light-harvesting antennae supercomplex," Nature Communications, Nature, vol. 15(1), pages 1-12, December.
    3. Songhao Zhao & Lili Shen & Xiaoyi Li & Qiushuang Tao & Zhenhua Li & Caizhe Xu & Cuicui Zhou & Yanyan Yang & Min Sang & Guangye Han & Long-Jiang Yu & Tingyun Kuang & Jian-Ren Shen & Wenda Wang, 2023. "Structural insights into photosystem II supercomplex and trimeric FCP antennae of a centric diatom Cyclotella meneghiniana," Nature Communications, Nature, vol. 14(1), pages 1-15, December.

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