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Cryo-EM and femtosecond spectroscopic studies provide mechanistic insight into the energy transfer in CpcL-phycobilisomes

Author

Listed:
  • Lvqin Zheng

    (Peking University
    Peking University)

  • Zhengdong Zhang

    (Peking University
    Peking University)

  • Hongrui Wang

    (Peking University
    Peking University)

  • Zhenggao Zheng

    (Peking University
    Peking University)

  • Jiayu Wang

    (Chinese Academy of Sciences)

  • Heyuan Liu

    (Chinese Academy of Sciences)

  • Hailong Chen

    (Chinese Academy of Sciences)

  • Chunxia Dong

    (Peking University
    Peking University)

  • Guopeng Wang

    (Peking University)

  • Yuxiang Weng

    (Chinese Academy of Sciences)

  • Ning Gao

    (Peking University
    Peking University)

  • Jindong Zhao

    (Peking University
    Peking University)

Abstract

Phycobilisomes (PBS) are the major light harvesting complexes of photosynthesis in the cyanobacteria and red algae. CpcL-PBS is a type of small PBS in cyanobacteria that transfers energy directly to photosystem I without the core structure. Here we report the cryo-EM structure of the CpcL-PBS from the cyanobacterium Synechocystis sp. PCC 6803 at 2.6-Å resolution. The structure shows the CpcD domain of ferredoxin: NADP+ oxidoreductase is located at the distal end of CpcL-PBS, responsible for its attachment to PBS. With the evidence of ultrafast transient absorption and fluorescence spectroscopy, the roles of individual bilins in energy transfer are revealed. The bilin 1Iβ822 located near photosystem I has an enhanced planarity and is the red-bilin responsible for the direct energy transfer to photosystem I.

Suggested Citation

  • Lvqin Zheng & Zhengdong Zhang & Hongrui Wang & Zhenggao Zheng & Jiayu Wang & Heyuan Liu & Hailong Chen & Chunxia Dong & Guopeng Wang & Yuxiang Weng & Ning Gao & Jindong Zhao, 2023. "Cryo-EM and femtosecond spectroscopic studies provide mechanistic insight into the energy transfer in CpcL-phycobilisomes," Nature Communications, Nature, vol. 14(1), pages 1-10, December.
    • Handle: RePEc:nat:natcom:v:14:y:2023:i:1:d:10.1038_s41467-023-39689-7
    DOI: 10.1038/s41467-023-39689-7
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    1. Yu-Zhong Zhang & Kang Li & Bing-Yue Qin & Jian-Ping Guo & Quan-Bao Zhang & Dian-Li Zhao & Xiu-Lan Chen & Jun Gao & Lu-Ning Liu & Long-Sheng Zhao, 2024. "Structure of cryptophyte photosystem II–light-harvesting antennae supercomplex," Nature Communications, Nature, vol. 15(1), pages 1-12, December.
    2. Han-Wei Jiang & Hsiang-Yi Wu & Chun-Hsiung Wang & Cheng-Han Yang & Jui-Tse Ko & Han-Chen Ho & Ming-Daw Tsai & Donald A. Bryant & Fay-Wei Li & Meng-Chiao Ho & Ming-Yang Ho, 2023. "A structure of the relict phycobilisome from a thylakoid-free cyanobacterium," Nature Communications, Nature, vol. 14(1), pages 1-14, December.

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