IDEAS home Printed from https://ideas.repec.org/a/nat/natcom/v15y2024i1d10.1038_s41467-024-46863-y.html
   My bibliography  Save this article

Thylakoid protein FPB1 synergistically cooperates with PAM68 to promote CP47 biogenesis and Photosystem II assembly

Author

Listed:
  • Lin Zhang

    (Shanghai Normal University)

  • Junxiang Ruan

    (Shanghai Normal University)

  • Fudan Gao

    (Shanghai Normal University)

  • Qiang Xin

    (Shanghai Normal University)

  • Li-Ping Che

    (Shanghai Normal University)

  • Lujuan Cai

    (Shanghai Normal University)

  • Zekun Liu

    (Shanghai Normal University)

  • Mengmeng Kong

    (Chinese Academy of Science)

  • Jean-David Rochaix

    (University of Geneva)

  • Hualing Mi

    (Chinese Academy of Science)

  • Lianwei Peng

    (Shanghai Normal University)

Abstract

In chloroplasts, insertion of proteins with multiple transmembrane domains (TMDs) into thylakoid membranes usually occurs in a co-translational manner. Here, we have characterized a thylakoid protein designated FPB1 (Facilitator of PsbB biogenesis1) which together with a previously reported factor PAM68 (Photosynthesis Affected Mutant68) is involved in assisting the biogenesis of CP47, a subunit of the Photosystem II (PSII) core. Analysis by ribosome profiling reveals increased ribosome stalling when the last TMD segment of CP47 emerges from the ribosomal tunnel in fpb1 and pam68. FPB1 interacts with PAM68 and both proteins coimmunoprecipitate with SecY/E and Alb3 as well as with some ribosomal components. Thus, our data indicate that, in coordination with the SecY/E translocon and the Alb3 integrase, FPB1 synergistically cooperates with PAM68 to facilitate the co-translational integration of the last two CP47 TMDs and the large loop between them into thylakoids and the PSII core complex.

Suggested Citation

  • Lin Zhang & Junxiang Ruan & Fudan Gao & Qiang Xin & Li-Ping Che & Lujuan Cai & Zekun Liu & Mengmeng Kong & Jean-David Rochaix & Hualing Mi & Lianwei Peng, 2024. "Thylakoid protein FPB1 synergistically cooperates with PAM68 to promote CP47 biogenesis and Photosystem II assembly," Nature Communications, Nature, vol. 15(1), pages 1-17, December.
    • Handle: RePEc:nat:natcom:v:15:y:2024:i:1:d:10.1038_s41467-024-46863-y
    DOI: 10.1038/s41467-024-46863-y
    as

    Download full text from publisher

    File URL: https://www.nature.com/articles/s41467-024-46863-y
    File Function: Abstract
    Download Restriction: no
    File URL: https://libkey.io/10.1038/s41467-024-46863-y?utm_source=ideas
    LibKey link: if access is restricted and if your library uses this service, LibKey will redirect you to where you can use your library subscription to access this item
    ---><---

    References listed on IDEAS

    as
    1. Yasufumi Umena & Keisuke Kawakami & Jian-Ren Shen & Nobuo Kamiya, 2011. "Crystal structure of oxygen-evolving photosystem II at a resolution of 1.9 Å," Nature, Nature, vol. 473(7345), pages 55-60, May.
    2. Ziyu Zhao & Irene Vercellino & Jana Knoppová & Roman Sobotka & James W. Murray & Peter J. Nixon & Leonid A. Sazanov & Josef Komenda, 2023. "The Ycf48 accessory factor occupies the site of the oxygen-evolving manganese cluster during photosystem II biogenesis," Nature Communications, Nature, vol. 14(1), pages 1-11, December.
    Full references (including those not matched with items on IDEAS)

    Most related items

    These are the items that most often cite the same works as this one and are cited by the same works as this one.
    1. Rana Hussein & Mohamed Ibrahim & Asmit Bhowmick & Philipp S. Simon & Ruchira Chatterjee & Louise Lassalle & Margaret Doyle & Isabel Bogacz & In-Sik Kim & Mun Hon Cheah & Sheraz Gul & Casper Lichtenber, 2021. "Structural dynamics in the water and proton channels of photosystem II during the S2 to S3 transition," Nature Communications, Nature, vol. 12(1), pages 1-16, December.
    2. Ziyu Zhao & Irene Vercellino & Jana Knoppová & Roman Sobotka & James W. Murray & Peter J. Nixon & Leonid A. Sazanov & Josef Komenda, 2023. "The Ycf48 accessory factor occupies the site of the oxygen-evolving manganese cluster during photosystem II biogenesis," Nature Communications, Nature, vol. 14(1), pages 1-11, December.
    3. Xuelei Pan & Mengyu Yan & Qian Liu & Xunbiao Zhou & Xiaobin Liao & Congli Sun & Jiexin Zhu & Callum McAleese & Pierre Couture & Matthew K. Sharpe & Richard Smith & Nianhua Peng & Jonathan England & Sh, 2024. "Electric-field-assisted proton coupling enhanced oxygen evolution reaction," Nature Communications, Nature, vol. 15(1), pages 1-11, December.
    4. Ryo Nagao & Koji Kato & Tasuku Hamaguchi & Yoshifumi Ueno & Naoki Tsuboshita & Shota Shimizu & Miyu Furutani & Shigeki Ehira & Yoshiki Nakajima & Keisuke Kawakami & Takehiro Suzuki & Naoshi Dohmae & S, 2023. "Structure of a monomeric photosystem I core associated with iron-stress-induced-A proteins from Anabaena sp. PCC 7120," Nature Communications, Nature, vol. 14(1), pages 1-13, December.
    5. Yusuke Yoneda & Eric A. Arsenault & Shiun-Jr Yang & Kaydren Orcutt & Masakazu Iwai & Graham R. Fleming, 2022. "The initial charge separation step in oxygenic photosynthesis," Nature Communications, Nature, vol. 13(1), pages 1-9, December.
    6. Koji Kato & Ryo Nagao & Yoshifumi Ueno & Makio Yokono & Takehiro Suzuki & Tian-Yi Jiang & Naoshi Dohmae & Fusamichi Akita & Seiji Akimoto & Naoyuki Miyazaki & Jian-Ren Shen, 2022. "Structure of a tetrameric photosystem I from a glaucophyte alga Cyanophora paradoxa," Nature Communications, Nature, vol. 13(1), pages 1-11, December.
    7. Shishang Dong & Guoqiang Huang & Changhui Wang & Jiajia Wang & Sen-Fang Sui & Xiaochun Qin, 2022. "Structure of the Acidobacteria homodimeric reaction center bound with cytochrome c," Nature Communications, Nature, vol. 13(1), pages 1-13, December.
    8. Ganesh, Ibram, 2015. "Solar fuels vis-à-vis electricity generation from sunlight: The current state-of-the-art (a review)," Renewable and Sustainable Energy Reviews, Elsevier, vol. 44(C), pages 904-932.
    9. Joseph T Snow & Despo Polyviou & Paul Skipp & Nathan A M Chrismas & Andrew Hitchcock & Richard Geider & C Mark Moore & Thomas S Bibby, 2015. "Quantifying Integrated Proteomic Responses to Iron Stress in the Globally Important Marine Diazotroph Trichodesmium," PLOS ONE, Public Library of Science, vol. 10(11), pages 1-24, November.
    10. Futing Zhang & Zuozhu Wen & Shanlin Wang & Weiyi Tang & Ya-Wei Luo & Sven A. Kranz & Haizheng Hong & Dalin Shi, 2022. "Phosphate limitation intensifies negative effects of ocean acidification on globally important nitrogen fixing cyanobacterium," Nature Communications, Nature, vol. 13(1), pages 1-13, December.
    11. Xiaojing Lin & Zhaojie Wang & Shoufu Cao & Yuying Hu & Siyuan Liu & Xiaodong Chen & Hongyu Chen & Xingheng Zhang & Shuxian Wei & Hui Xu & Zhi Cheng & Qi Hou & Daofeng Sun & Xiaoqing Lu, 2023. "Bioinspired trimesic acid anchored electrocatalysts with unique static and dynamic compatibility for enhanced water oxidation," Nature Communications, Nature, vol. 14(1), pages 1-10, December.
    12. Vincenzo Mascoli & Ahmad Farhan Bhatti & Luca Bersanini & Herbert Amerongen & Roberta Croce, 2022. "The antenna of far-red absorbing cyanobacteria increases both absorption and quantum efficiency of Photosystem II," Nature Communications, Nature, vol. 13(1), pages 1-8, December.
    13. Ryan Puskar & Chloe Truong & Kyle Swain & Saborni Chowdhury & Ka-Yi Chan & Shan Li & Kai-Wen Cheng & Ting Yu Wang & Yu-Ping Poh & Yuval Mazor & Haijun Liu & Tsui-Fen Chou & Brent L. Nannenga & Po-Lin , 2022. "Molecular asymmetry of a photosynthetic supercomplex from green sulfur bacteria," Nature Communications, Nature, vol. 13(1), pages 1-12, December.

    More about this item

    Statistics

    Access and download statistics

    Corrections

    All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:nat:natcom:v:15:y:2024:i:1:d:10.1038_s41467-024-46863-y. See general information about how to correct material in RePEc.

    If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.

    If CitEc recognized a bibliographic reference but did not link an item in RePEc to it, you can help with this form .

    If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.

    For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: Sonal Shukla or Springer Nature Abstracting and Indexing (email available below). General contact details of provider: http://www.nature.com .

    Please note that corrections may take a couple of weeks to filter through the various RePEc services.

    IDEAS is a RePEc service. RePEc uses bibliographic data supplied by the respective publishers.