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Chemical reaction-mediated covalent localization of bacteria

Author

Listed:
  • Huilong Luo

    (Shanghai Jiao Tong University)

  • Yanmei Chen

    (Shanghai Jiao Tong University)

  • Xiao Kuang

    (Shanghai Jiao Tong University)

  • Xinyue Wang

    (Shanghai Jiao Tong University)

  • Fengmin Yang

    (Shanghai Jiao Tong University)

  • Zhenping Cao

    (Shanghai Jiao Tong University)

  • Lu Wang

    (Shanghai Jiao Tong University)

  • Sisi Lin

    (Shanghai Jiao Tong University)

  • Feng Wu

    (Shanghai Jiao Tong University)

  • Jinyao Liu

    (Shanghai Jiao Tong University)

Abstract

Methods capable of manipulating bacterial colonization are of great significance for modulating host-microbiota relationships. Here, we describe a strategy of in-situ chemical reaction-mediated covalent localization of bacteria. Through a simple one-step imidoester reaction, primary amino groups on bacterial surface can be converted to free thiols under cytocompatible conditions. Surface thiolation is applicable to modify diverse strains and the number of introduced thiols per bacterium can be easily tuned by varying feed ratios. These chemically reactive bacteria are able to spontaneously bond with mucous layer by catalyst-free thiol-disulfide exchange between mucin-associated disulfides and newly converted thiols on bacterial surface and show thiolation level-dependent attachment. Bacteria optimized with 9.3 × 107 thiols per cell achieve 170-fold higher attachment in mucin-enriched jejunum, a challenging location for gut microbiota to colonize. As a proof-of-concept application for microbiota transplantation, covalent bonding-assisted localization of an oral probiotic in the jejunum generates an improved remission of jejunal mucositis. Our findings demonstrate that transforming bacteria with a reactive surface provides an approach to chemically control bacterial localization, which is highly desirable for developing next-generation bacterial living bioagents.

Suggested Citation

  • Huilong Luo & Yanmei Chen & Xiao Kuang & Xinyue Wang & Fengmin Yang & Zhenping Cao & Lu Wang & Sisi Lin & Feng Wu & Jinyao Liu, 2022. "Chemical reaction-mediated covalent localization of bacteria," Nature Communications, Nature, vol. 13(1), pages 1-13, December.
    • Handle: RePEc:nat:natcom:v:13:y:2022:i:1:d:10.1038_s41467-022-35579-6
    DOI: 10.1038/s41467-022-35579-6
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    References listed on IDEAS

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    1. Maximilian M. Sauer & Roman P. Jakob & Jonathan Eras & Sefer Baday & Deniz Eriş & Giulio Navarra & Simon Bernèche & Beat Ernst & Timm Maier & Rudi Glockshuber, 2016. "Catch-bond mechanism of the bacterial adhesin FimH," Nature Communications, Nature, vol. 7(1), pages 1-13, April.
    2. Zhenping Cao & Xinyue Wang & Yan Pang & Shanshan Cheng & Jinyao Liu, 2019. "Biointerfacial self-assembly generates lipid membrane coated bacteria for enhanced oral delivery and treatment," Nature Communications, Nature, vol. 10(1), pages 1-11, December.
    3. Andrea Canal-Martín & Ruth Pérez-Fernández, 2021. "Biomimetic selenocystine based dynamic combinatorial chemistry for thiol-disulfide exchange," Nature Communications, Nature, vol. 12(1), pages 1-12, December.
    4. Minsoo Kim & Michinaga Ogawa & Yukihiro Fujita & Yuko Yoshikawa & Takeshi Nagai & Tomohiro Koyama & Shinya Nagai & Anika Lange & Reinhard Fässler & Chihiro Sasakawa, 2009. "Bacteria hijack integrin-linked kinase to stabilize focal adhesions and block cell detachment," Nature, Nature, vol. 459(7246), pages 578-582, May.
    5. Elisabeth Serger & Lucia Luengo-Gutierrez & Jessica S. Chadwick & Guiping Kong & Luming Zhou & Greg Crawford & Matt C. Danzi & Antonis Myridakis & Alexander Brandis & Adesola Temitope Bello & Franzisk, 2022. "The gut metabolite indole-3 propionate promotes nerve regeneration and repair," Nature, Nature, vol. 607(7919), pages 585-592, July.
    6. Zhongmin Geng & Zhenping Cao & Rui Liu & Ke Liu & Jinyao Liu & Weihong Tan, 2021. "Aptamer-assisted tumor localization of bacteria for enhanced biotherapy," Nature Communications, Nature, vol. 12(1), pages 1-12, December.
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