IDEAS home Printed from https://ideas.repec.org/a/nat/natcom/v15y2024i1d10.1038_s41467-024-48128-0.html
   My bibliography  Save this article

Targeted delivery of the probiotic Saccharomyces boulardii to the extracellular matrix enhances gut residence time and recovery in murine colitis

Author

Listed:
  • Mairead K. Heavey

    (University of North Carolina at Chapel Hill)

  • Anthony Hazelton

    (University of North Carolina at Chapel Hill)

  • Yuyan Wang

    (University of North Carolina at Chapel Hill)

  • Mitzy Garner

    (University of North Carolina at Chapel Hill)

  • Aaron C. Anselmo

    (University of North Carolina at Chapel Hill
    VitaKey Incorporation)

  • Janelle C. Arthur

    (The University of North Carolina at Chapel Hill
    The University of North Carolina at Chapel Hill
    The University of North Carolina at Chapel Hill)

  • Juliane Nguyen

    (University of North Carolina at Chapel Hill
    The University of North Carolina at Chapel Hill)

Abstract

Probiotic and engineered microbe-based therapeutics are an emerging class of pharmaceutical agents. They represent a promising strategy for treating various chronic and inflammatory conditions by interacting with the host immune system and/or delivering therapeutic molecules. Here, we engineered a targeted probiotic yeast platform wherein Saccharomyces boulardii is designed to bind to abundant extracellular matrix proteins found within inflammatory lesions of the gastrointestinal tract through tunable antibody surface display. This approach enabled an additional 24–48 h of probiotic gut residence time compared to controls and 100-fold increased probiotic concentrations within the colon in preclinical models of ulcerative colitis in female mice. As a result, pharmacodynamic parameters including colon length, colonic cytokine expression profiles, and histological inflammation scores were robustly improved and restored back to healthy levels. Overall, these studies highlight the potential for targeted microbial therapeutics as a potential oral dosage form for the treatment of inflammatory bowel diseases.

Suggested Citation

  • Mairead K. Heavey & Anthony Hazelton & Yuyan Wang & Mitzy Garner & Aaron C. Anselmo & Janelle C. Arthur & Juliane Nguyen, 2024. "Targeted delivery of the probiotic Saccharomyces boulardii to the extracellular matrix enhances gut residence time and recovery in murine colitis," Nature Communications, Nature, vol. 15(1), pages 1-16, December.
    • Handle: RePEc:nat:natcom:v:15:y:2024:i:1:d:10.1038_s41467-024-48128-0
    DOI: 10.1038/s41467-024-48128-0
    as

    Download full text from publisher

    File URL: https://www.nature.com/articles/s41467-024-48128-0
    File Function: Abstract
    Download Restriction: no
    File URL: https://libkey.io/10.1038/s41467-024-48128-0?utm_source=ideas
    LibKey link: if access is restricted and if your library uses this service, LibKey will redirect you to where you can use your library subscription to access this item
    ---><---

    References listed on IDEAS

    as
    1. Zhenping Cao & Xinyue Wang & Yan Pang & Shanshan Cheng & Jinyao Liu, 2019. "Biointerfacial self-assembly generates lipid membrane coated bacteria for enhanced oral delivery and treatment," Nature Communications, Nature, vol. 10(1), pages 1-11, December.
    2. Fernando P. Canale & Camilla Basso & Gaia Antonini & Michela Perotti & Ning Li & Anna Sokolovska & Julia Neumann & Michael J. James & Stefania Geiger & Wenjie Jin & Jean-Philippe Theurillat & Kip A. W, 2021. "Metabolic modulation of tumours with engineered bacteria for immunotherapy," Nature, Nature, vol. 598(7882), pages 662-666, October.
    3. Elvin Koh & In Young Hwang & Hui Ling Lee & Ryan De Sotto & Jonathan Wei Jie Lee & Yung Seng Lee & John C. March & Matthew Wook Chang, 2022. "Engineering probiotics to inhibit Clostridioides difficile infection by dynamic regulation of intestinal metabolism," Nature Communications, Nature, vol. 13(1), pages 1-13, December.
    Full references (including those not matched with items on IDEAS)

    Most related items

    These are the items that most often cite the same works as this one and are cited by the same works as this one.
    1. Zhongmin Geng & Zhenping Cao & Rui Liu & Ke Liu & Jinyao Liu & Weihong Tan, 2021. "Aptamer-assisted tumor localization of bacteria for enhanced biotherapy," Nature Communications, Nature, vol. 12(1), pages 1-12, December.
    2. Huilong Luo & Yanmei Chen & Xiao Kuang & Xinyue Wang & Fengmin Yang & Zhenping Cao & Lu Wang & Sisi Lin & Feng Wu & Jinyao Liu, 2022. "Chemical reaction-mediated covalent localization of bacteria," Nature Communications, Nature, vol. 13(1), pages 1-13, December.
    3. Yi Zhang & Mingjie Wang & Ling Ye & Shengqi Shen & Yuxi Zhang & Xiaoyu Qian & Tong Zhang & Mengqiu Yuan & Zijian Ye & Jin Cai & Xiang Meng & Shiqiao Qiu & Shengzhi Liu & Rui Liu & Weidong Jia & Xianzh, 2024. "HKDC1 promotes tumor immune evasion in hepatocellular carcinoma by coupling cytoskeleton to STAT1 activation and PD-L1 expression," Nature Communications, Nature, vol. 15(1), pages 1-14, December.
    4. Xiaotu Ma & Xiaolong Liang & Yao Li & Qingqing Feng & Keman Cheng & Nana Ma & Fei Zhu & Xinjing Guo & Yale Yue & Guangna Liu & Tianjiao Zhang & Jie Liang & Lei Ren & Xiao Zhao & Guangjun Nie, 2023. "Modular-designed engineered bacteria for precision tumor immunotherapy via spatiotemporal manipulation by magnetic field," Nature Communications, Nature, vol. 14(1), pages 1-22, December.
    5. Ling Huang & Wei Tang & Lina He & Mengke Li & Xian Lin & Ao Hu & Xindi Huang & Zhouyu Wu & Zhiyong Wu & Shiyun Chen & Yangbo Hu, 2024. "Engineered probiotic Escherichia coli elicits immediate and long-term protection against influenza A virus in mice," Nature Communications, Nature, vol. 15(1), pages 1-16, December.
    6. Jae Sung Cho & Dongsoo Yang & Cindy Pricilia Surya Prabowo & Mohammad Rifqi Ghiffary & Taehee Han & Kyeong Rok Choi & Cheon Woo Moon & Hengrui Zhou & Jae Yong Ryu & Hyun Uk Kim & Sang Yup Lee, 2023. "Targeted and high-throughput gene knockdown in diverse bacteria using synthetic sRNAs," Nature Communications, Nature, vol. 14(1), pages 1-13, December.
    7. Jun Zhou & Maoyi Li & Qiufang Chen & Xinjie Li & Linfu Chen & Ziliang Dong & Wenjun Zhu & Yang Yang & Zhuang Liu & Qian Chen, 2022. "Programmable probiotics modulate inflammation and gut microbiota for inflammatory bowel disease treatment after effective oral delivery," Nature Communications, Nature, vol. 13(1), pages 1-14, December.
    8. Jiezhou Pan & Guidong Gong & Qin Wang & Jiaojiao Shang & Yunxiang He & Chelsea Catania & Dan Birnbaum & Yifei Li & Zhijun Jia & Yaoyao Zhang & Neel S. Joshi & Junling Guo, 2022. "A single-cell nanocoating of probiotics for enhanced amelioration of antibiotic-associated diarrhea," Nature Communications, Nature, vol. 13(1), pages 1-11, December.
    9. Dinh-Huy Nguyen & Ari Chong & Yeongjin Hong & Jung-Joon Min, 2023. "Bioengineering of bacteria for cancer immunotherapy," Nature Communications, Nature, vol. 14(1), pages 1-5, December.
    10. Wenjing Jin & Xianfeng Lin & Haihua Pan & Chenchen Zhao & Pengcheng Qiu & Ruibo Zhao & Zihe Hu & Yanyan Zhou & Haiyan Wu & Xiao Chen & Hongwei Ouyang & Zhijian Xie & Ruikang Tang, 2021. "Engineered osteoclasts as living treatment materials for heterotopic ossification therapy," Nature Communications, Nature, vol. 12(1), pages 1-12, December.

    More about this item

    Statistics

    Access and download statistics

    Corrections

    All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:nat:natcom:v:15:y:2024:i:1:d:10.1038_s41467-024-48128-0. See general information about how to correct material in RePEc.

    If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.

    If CitEc recognized a bibliographic reference but did not link an item in RePEc to it, you can help with this form .

    If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.

    For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: Sonal Shukla or Springer Nature Abstracting and Indexing (email available below). General contact details of provider: http://www.nature.com .

    Please note that corrections may take a couple of weeks to filter through the various RePEc services.

    IDEAS is a RePEc service. RePEc uses bibliographic data supplied by the respective publishers.