IDEAS home Printed from https://ideas.repec.org/a/nat/natcom/v13y2022i1d10.1038_s41467-022-29672-z.html
   My bibliography  Save this article

A single-cell nanocoating of probiotics for enhanced amelioration of antibiotic-associated diarrhea

Author

Listed:
  • Jiezhou Pan

    (BMI Center for Biomass Materials and Nanointerfaces, College of Biomass Science and Engineering, West China Second University Hospital, Sichuan University)

  • Guidong Gong

    (BMI Center for Biomass Materials and Nanointerfaces, College of Biomass Science and Engineering, West China Second University Hospital, Sichuan University)

  • Qin Wang

    (School of Pharmacy, Southwest Minzu University)

  • Jiaojiao Shang

    (BMI Center for Biomass Materials and Nanointerfaces, College of Biomass Science and Engineering, West China Second University Hospital, Sichuan University)

  • Yunxiang He

    (BMI Center for Biomass Materials and Nanointerfaces, College of Biomass Science and Engineering, West China Second University Hospital, Sichuan University)

  • Chelsea Catania

    (Massachusetts Institute of Technology)

  • Dan Birnbaum

    (Wyss Institute for Biologically Inspired Engineering, John A. Paulson School of Engineering and Applied Sciences, Harvard University)

  • Yifei Li

    (BMI Center for Biomass Materials and Nanointerfaces, College of Biomass Science and Engineering, West China Second University Hospital, Sichuan University
    Key Laboratory of Birth Defects and Related of Women and Children of Ministry of Education, Department of Pediatrics, The Reproductive Medical Center, Department of Obstetrics and Gynecology, West China Second University Hospital, Sichuan University)

  • Zhijun Jia

    (BMI Center for Biomass Materials and Nanointerfaces, College of Biomass Science and Engineering, West China Second University Hospital, Sichuan University
    Key Laboratory of Birth Defects and Related of Women and Children of Ministry of Education, Department of Pediatrics, The Reproductive Medical Center, Department of Obstetrics and Gynecology, West China Second University Hospital, Sichuan University
    West China School of Pharmacy, Sichuan University)

  • Yaoyao Zhang

    (BMI Center for Biomass Materials and Nanointerfaces, College of Biomass Science and Engineering, West China Second University Hospital, Sichuan University
    Key Laboratory of Birth Defects and Related of Women and Children of Ministry of Education, Department of Pediatrics, The Reproductive Medical Center, Department of Obstetrics and Gynecology, West China Second University Hospital, Sichuan University)

  • Neel S. Joshi

    (Wyss Institute for Biologically Inspired Engineering, John A. Paulson School of Engineering and Applied Sciences, Harvard University
    Northeastern University)

  • Junling Guo

    (BMI Center for Biomass Materials and Nanointerfaces, College of Biomass Science and Engineering, West China Second University Hospital, Sichuan University
    Wyss Institute for Biologically Inspired Engineering, John A. Paulson School of Engineering and Applied Sciences, Harvard University
    State Key Laboratory of Polymer Materials Engineering, Sichuan University)

Abstract

The gut microbiota represents a large community of microorganisms that play an important role in immune regulation and maintenance of homeostasis. Living bacteria receive increasing interest as potential therapeutics for gut disorders, because they inhibit the colonization of pathogens and positively regulate the composition of bacteria in gut. However, these treatments are often accompanied by antibiotic administration targeting pathogens. In these cases, the efficacy of therapeutic bacteria is compromised by their susceptibility to antibiotics. Here, we demonstrate that a single-cell coating composed of tannic acids and ferric ions, referred to as ‘nanoarmor’, can protect bacteria from the action of antibiotics. The nanoarmor protects both Gram-positive and Gram-negative bacteria against six clinically relevant antibiotics. The multiple interactions between the nanoarmor and antibiotic molecules allow the antibiotics to be effectively absorbed onto the nanoarmor. Armored probiotics have shown the ability to colonize inside the gastrointestinal tracts of levofloxacin-treated rats, which significantly reduced antibiotic-associated diarrhea (AAD) resulting from the levofloxacin-treatment and improved some of the pre-inflammatory symptoms caused by AAD. This nanoarmor strategy represents a robust platform to enhance the potency of therapeutic bacteria in the gastrointestinal tracts of patients receiving antibiotics and to avoid the negative effects of antibiotics in the gastrointestinal tract.

Suggested Citation

  • Jiezhou Pan & Guidong Gong & Qin Wang & Jiaojiao Shang & Yunxiang He & Chelsea Catania & Dan Birnbaum & Yifei Li & Zhijun Jia & Yaoyao Zhang & Neel S. Joshi & Junling Guo, 2022. "A single-cell nanocoating of probiotics for enhanced amelioration of antibiotic-associated diarrhea," Nature Communications, Nature, vol. 13(1), pages 1-11, December.
  • Handle: RePEc:nat:natcom:v:13:y:2022:i:1:d:10.1038_s41467-022-29672-z
    DOI: 10.1038/s41467-022-29672-z
    as

    Download full text from publisher

    File URL: https://www.nature.com/articles/s41467-022-29672-z
    File Function: Abstract
    Download Restriction: no

    File URL: https://libkey.io/10.1038/s41467-022-29672-z?utm_source=ideas
    LibKey link: if access is restricted and if your library uses this service, LibKey will redirect you to where you can use your library subscription to access this item
    ---><---

    References listed on IDEAS

    as
    1. Zhenping Cao & Xinyue Wang & Yan Pang & Shanshan Cheng & Jinyao Liu, 2019. "Biointerfacial self-assembly generates lipid membrane coated bacteria for enhanced oral delivery and treatment," Nature Communications, Nature, vol. 10(1), pages 1-11, December.
    2. Pipat Piewngam & Yue Zheng & Thuan H. Nguyen & Seth W. Dickey & Hwang-Soo Joo & Amer E. Villaruz & Kyle A. Glose & Emilie L. Fisher & Rachelle L. Hunt & Barry Li & Janice Chiou & Sujiraphong Pharkjaks, 2018. "Pathogen elimination by probiotic Bacillus via signalling interference," Nature, Nature, vol. 562(7728), pages 532-537, October.
    3. Rishi Drolia & Mary Anne Roshni Amalaradjou & Valerie Ryan & Shivendra Tenguria & Dongqi Liu & Xingjian Bai & Luping Xu & Atul K. Singh & Abigail D. Cox & Victor Bernal-Crespo & James A. Schaber & Bru, 2020. "Receptor-targeted engineered probiotics mitigate lethal Listeria infection," Nature Communications, Nature, vol. 11(1), pages 1-23, December.
    4. Eran Blacher & Stavros Bashiardes & Hagit Shapiro & Daphna Rothschild & Uria Mor & Mally Dori-Bachash & Christian Kleimeyer & Claudia Moresi & Yotam Harnik & Maya Zur & Michal Zabari & Rotem Ben-Zeev , 2019. "Potential roles of gut microbiome and metabolites in modulating ALS in mice," Nature, Nature, vol. 572(7770), pages 474-480, August.
    5. Hiroaki Konishi & Mikihiro Fujiya & Hiroki Tanaka & Nobuhiro Ueno & Kentaro Moriichi & Junpei Sasajima & Katsuya Ikuta & Hiroaki Akutsu & Hiroki Tanabe & Yutaka Kohgo, 2016. "Probiotic-derived ferrichrome inhibits colon cancer progression via JNK-mediated apoptosis," Nature Communications, Nature, vol. 7(1), pages 1-12, November.
    Full references (including those not matched with items on IDEAS)

    Most related items

    These are the items that most often cite the same works as this one and are cited by the same works as this one.
    1. Monica Lopes-Ferreira & Adolfo Luis Almeida Maleski & Leticia Balan-Lima & Jefferson Thiago Gonçalves Bernardo & Lucas Marques Hipolito & Ana Carolina Seni-Silva & Joao Batista-Filho & Maria Alice Pim, 2022. "Impact of Pesticides on Human Health in the Last Six Years in Brazil," IJERPH, MDPI, vol. 19(6), pages 1-19, March.
    2. Mairead K. Heavey & Anthony Hazelton & Yuyan Wang & Mitzy Garner & Aaron C. Anselmo & Janelle C. Arthur & Juliane Nguyen, 2024. "Targeted delivery of the probiotic Saccharomyces boulardii to the extracellular matrix enhances gut residence time and recovery in murine colitis," Nature Communications, Nature, vol. 15(1), pages 1-16, December.
    3. Zhongmin Geng & Zhenping Cao & Rui Liu & Ke Liu & Jinyao Liu & Weihong Tan, 2021. "Aptamer-assisted tumor localization of bacteria for enhanced biotherapy," Nature Communications, Nature, vol. 12(1), pages 1-12, December.
    4. Huilong Luo & Yanmei Chen & Xiao Kuang & Xinyue Wang & Fengmin Yang & Zhenping Cao & Lu Wang & Sisi Lin & Feng Wu & Jinyao Liu, 2022. "Chemical reaction-mediated covalent localization of bacteria," Nature Communications, Nature, vol. 13(1), pages 1-13, December.
    5. Felipe Papa Pellizoni & Aline Zazeri Leite & Nathália de Campos Rodrigues & Marcelo Jordão Ubaiz & Marina Ignácio Gonzaga & Nauyta Naomi Campos Takaoka & Vânia Sammartino Mariano & Wellington Pine Omo, 2021. "Detection of Dysbiosis and Increased Intestinal Permeability in Brazilian Patients with Relapsing–Remitting Multiple Sclerosis," IJERPH, MDPI, vol. 18(9), pages 1-17, April.
    6. Xiaotu Ma & Xiaolong Liang & Yao Li & Qingqing Feng & Keman Cheng & Nana Ma & Fei Zhu & Xinjing Guo & Yale Yue & Guangna Liu & Tianjiao Zhang & Jie Liang & Lei Ren & Xiao Zhao & Guangjun Nie, 2023. "Modular-designed engineered bacteria for precision tumor immunotherapy via spatiotemporal manipulation by magnetic field," Nature Communications, Nature, vol. 14(1), pages 1-22, December.
    7. Jun Zhou & Maoyi Li & Qiufang Chen & Xinjie Li & Linfu Chen & Ziliang Dong & Wenjun Zhu & Yang Yang & Zhuang Liu & Qian Chen, 2022. "Programmable probiotics modulate inflammation and gut microbiota for inflammatory bowel disease treatment after effective oral delivery," Nature Communications, Nature, vol. 13(1), pages 1-14, December.
    8. Wenjing Jin & Xianfeng Lin & Haihua Pan & Chenchen Zhao & Pengcheng Qiu & Ruibo Zhao & Zihe Hu & Yanyan Zhou & Haiyan Wu & Xiao Chen & Hongwei Ouyang & Zhijian Xie & Ruikang Tang, 2021. "Engineered osteoclasts as living treatment materials for heterotopic ossification therapy," Nature Communications, Nature, vol. 12(1), pages 1-12, December.
    9. Irene Soffritti & Maria D’Accolti & Luca Lanzoni & Antonella Volta & Matteo Bisi & Sante Mazzacane & Elisabetta Caselli, 2019. "The Potential Use of Microorganisms as Restorative Agents: An Update," Sustainability, MDPI, vol. 11(14), pages 1-17, July.

    More about this item

    Statistics

    Access and download statistics

    Corrections

    All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:nat:natcom:v:13:y:2022:i:1:d:10.1038_s41467-022-29672-z. See general information about how to correct material in RePEc.

    If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.

    If CitEc recognized a bibliographic reference but did not link an item in RePEc to it, you can help with this form .

    If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.

    For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: Sonal Shukla or Springer Nature Abstracting and Indexing (email available below). General contact details of provider: http://www.nature.com .

    Please note that corrections may take a couple of weeks to filter through the various RePEc services.

    IDEAS is a RePEc service. RePEc uses bibliographic data supplied by the respective publishers.