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A multi-state dynamic process confers mechano-adaptation to a biological nanomachine

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  • Navish Wadhwa

    (Arizona State University
    Arizona State University)

  • Alberto Sassi

    (IBM T. J. Watson Research Center)

  • Howard C. Berg

    (Harvard University)

  • Yuhai Tu

    (IBM T. J. Watson Research Center)

Abstract

Adaptation is a defining feature of living systems. The bacterial flagellar motor adapts to changes in the external mechanical load by adding or removing torque-generating (stator) units. But the molecular mechanism behind this mechano-adaptation remains unclear. Here, we combine single motor eletrorotation experiments and theoretical modeling to show that mechano-adaptation of the flagellar motor is enabled by multiple mechanosensitive internal states. Dwell time statistics from experiments suggest the existence of at least two bound states with a high and a low unbinding rate, respectively. A first-passage-time analysis of a four-state model quantitatively explains the experimental data and determines the transition rates among all four states. The torque generated by bound stator units controls their effective unbinding rate by modulating the transition between the bound states, possibly via a catch bond mechanism. Similar force-mediated feedback enabled by multiple internal states may apply to adaptation in other macromolecular complexes.

Suggested Citation

  • Navish Wadhwa & Alberto Sassi & Howard C. Berg & Yuhai Tu, 2022. "A multi-state dynamic process confers mechano-adaptation to a biological nanomachine," Nature Communications, Nature, vol. 13(1), pages 1-9, December.
  • Handle: RePEc:nat:natcom:v:13:y:2022:i:1:d:10.1038_s41467-022-33075-5
    DOI: 10.1038/s41467-022-33075-5
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    References listed on IDEAS

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    1. Haidai Hu & Philipp F. Popp & Mònica Santiveri & Aritz Roa-Eguiara & Yumeng Yan & Freddie J. O. Martin & Zheyi Liu & Navish Wadhwa & Yong Wang & Marc Erhardt & Nicholas M. I. Taylor, 2023. "Ion selectivity and rotor coupling of the Vibrio flagellar sodium-driven stator unit," Nature Communications, Nature, vol. 14(1), pages 1-14, December.

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