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Tagging active neurons by soma-targeted Cal-Light

Author

Listed:
  • Jung Ho Hyun

    (Johns Hopkins School of Medicine
    Max Planck Florida Institute for Neuroscience
    Department of Brain Sciences, DGIST)

  • Kenichiro Nagahama

    (Johns Hopkins School of Medicine)

  • Ho Namkung

    (Johns Hopkins School of Medicine)

  • Neymi Mignocchi

    (Max Planck Florida Institute for Neuroscience)

  • Seung-Eon Roh

    (Johns Hopkins School of Medicine)

  • Patrick Hannan

    (Johns Hopkins School of Medicine
    Max Planck Florida Institute for Neuroscience)

  • Sarah Krüssel

    (Johns Hopkins School of Medicine
    Max Planck Florida Institute for Neuroscience)

  • Chuljung Kwak

    (Johns Hopkins School of Medicine)

  • Abigail McElroy

    (Johns Hopkins School of Medicine)

  • Bian Liu

    (Johns Hopkins School of Medicine)

  • Mingguang Cui

    (Korea University College of Medicine
    Korea University College of Medicine)

  • Seunghwan Lee

    (Korea University College of Medicine
    Korea University College of Medicine)

  • Dongmin Lee

    (Korea University College of Medicine
    Korea University College of Medicine)

  • Richard L. Huganir

    (Johns Hopkins School of Medicine)

  • Paul F. Worley

    (Johns Hopkins School of Medicine)

  • Akira Sawa

    (Johns Hopkins School of Medicine
    Johns Hopkins School of Medicine
    Johns Hopkins School of Medicine
    Johns Hopkins School of Medicine)

  • Hyung-Bae Kwon

    (Johns Hopkins School of Medicine
    Max Planck Florida Institute for Neuroscience
    Johns Hopkins School of Medicine)

Abstract

Verifying causal effects of neural circuits is essential for proving a direct circuit-behavior relationship. However, techniques for tagging only active neurons with high spatiotemporal precision remain at the beginning stages. Here we develop the soma-targeted Cal-Light (ST-Cal-Light) which selectively converts somatic calcium rise triggered by action potentials into gene expression. Such modification simultaneously increases the signal-to-noise ratio of reporter gene expression and reduces the light requirement for successful labeling. Because of the enhanced efficacy, the ST-Cal-Light enables the tagging of functionally engaged neurons in various forms of behaviors, including context-dependent fear conditioning, lever-pressing choice behavior, and social interaction behaviors. We also target kainic acid-sensitive neuronal populations in the hippocampus which subsequently suppress seizure symptoms, suggesting ST-Cal-Light’s applicability in controlling disease-related neurons. Furthermore, the generation of a conditional ST-Cal-Light knock-in mouse provides an opportunity to tag active neurons in a region- or cell-type specific manner via crossing with other Cre-driver lines. Thus, the versatile ST-Cal-Light system links somatic action potentials to behaviors with high temporal precision, and ultimately allows functional circuit dissection at a single cell resolution.

Suggested Citation

  • Jung Ho Hyun & Kenichiro Nagahama & Ho Namkung & Neymi Mignocchi & Seung-Eon Roh & Patrick Hannan & Sarah Krüssel & Chuljung Kwak & Abigail McElroy & Bian Liu & Mingguang Cui & Seunghwan Lee & Dongmin, 2022. "Tagging active neurons by soma-targeted Cal-Light," Nature Communications, Nature, vol. 13(1), pages 1-16, December.
  • Handle: RePEc:nat:natcom:v:13:y:2022:i:1:d:10.1038_s41467-022-35406-y
    DOI: 10.1038/s41467-022-35406-y
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    References listed on IDEAS

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