IDEAS home Printed from https://ideas.repec.org/a/nat/nature/v408y2000i6812d10.1038_35046076.html
   My bibliography  Save this article

Analysis of calcium channels in single spines using optical fluctuation analysis

Author

Listed:
  • Bernardo L. Sabatini

    (Howard Hughes Medical Institute, Cold Spring Harbor Laboratory)

  • Karel Svoboda

    (Howard Hughes Medical Institute, Cold Spring Harbor Laboratory)

Abstract

Most synapses form on small, specialized postsynaptic structures known as dendritic spines1. The influx of Ca2+ ions into such spines—through synaptic receptors and voltage-sensitive Ca2+ channels (VSCCs)—triggers diverse processes that underlie synaptic plasticity2. Using two-photon laser scanning microscopy3, we imaged action-potential-induced transient changes in Ca2+ concentration in spines and dendrites of CA1 pyramidal neurons in rat hippocampal slices4. Through analysis of the large trial-to-trial fluctuations in these transients, we have determined the number and properties of VSCCs in single spines. Here we report that each spine contains 1–20 VSCCs, and that this number increases with spine volume. We are able to detect the opening of a single VSCC on a spine. In spines located on the proximal dendritic tree, VSCCs normally open with high probability (∼0.5) following dendritic action potentials. Activation of GABAB receptors reduced this probability in apical spines to ∼0.3 but had no effect on VSCCs in dendrites or basal spines. Our studies show that the spatial distribution of VSCC subtypes and their modulatory potential is regulated with submicrometre precision.

Suggested Citation

  • Bernardo L. Sabatini & Karel Svoboda, 2000. "Analysis of calcium channels in single spines using optical fluctuation analysis," Nature, Nature, vol. 408(6812), pages 589-593, November.
    • Handle: RePEc:nat:nature:v:408:y:2000:i:6812:d:10.1038_35046076
    DOI: 10.1038/35046076
    as

    Download full text from publisher

    File URL: https://www.nature.com/articles/35046076
    File Function: Abstract
    Download Restriction: Access to the full text of the articles in this series is restricted.
    File URL: https://libkey.io/10.1038/35046076?utm_source=ideas
    LibKey link: if access is restricted and if your library uses this service, LibKey will redirect you to where you can use your library subscription to access this item
    ---><---

    As the access to this document is restricted, you may want to search for a different version of it.

    Citations

    Citations are extracted by the CitEc Project, subscribe to its RSS feed for this item.
    as


    Cited by:

    1. Friedrich W Johenning & Anne-Kathrin Theis & Ulrike Pannasch & Martin Rückl & Sten Rüdiger & Dietmar Schmitz, 2015. "Ryanodine Receptor Activation Induces Long-Term Plasticity of Spine Calcium Dynamics," PLOS Biology, Public Library of Science, vol. 13(6), pages 1-29, June.
    2. Jung Ho Hyun & Kenichiro Nagahama & Ho Namkung & Neymi Mignocchi & Seung-Eon Roh & Patrick Hannan & Sarah Krüssel & Chuljung Kwak & Abigail McElroy & Bian Liu & Mingguang Cui & Seunghwan Lee & Dongmin, 2022. "Tagging active neurons by soma-targeted Cal-Light," Nature Communications, Nature, vol. 13(1), pages 1-16, December.

    More about this item

    Statistics

    Access and download statistics

    Corrections

    All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:nat:nature:v:408:y:2000:i:6812:d:10.1038_35046076. See general information about how to correct material in RePEc.

    If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.

    We have no bibliographic references for this item. You can help adding them by using this form .

    If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.

    For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: Sonal Shukla or Springer Nature Abstracting and Indexing (email available below). General contact details of provider: http://www.nature.com .

    Please note that corrections may take a couple of weeks to filter through the various RePEc services.

    IDEAS is a RePEc service. RePEc uses bibliographic data supplied by the respective publishers.