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Multiplex base editing to convert TAG into TAA codons in the human genome

Author

Listed:
  • Yuting Chen

    (Harvard Medical School
    Wyss Institute for Biologically Inspired Engineering
    Chinese Academy of Sciences)

  • Eriona Hysolli

    (Harvard Medical School
    Wyss Institute for Biologically Inspired Engineering)

  • Anlu Chen

    (Harvard Medical School)

  • Stephen Casper

    (Harvard Medical School)

  • Songlei Liu

    (Harvard Medical School
    Wyss Institute for Biologically Inspired Engineering)

  • Kevin Yang

    (Harvard Medical School)

  • Chenli Liu

    (Chinese Academy of Sciences)

  • George Church

    (Harvard Medical School
    Wyss Institute for Biologically Inspired Engineering)

Abstract

Whole-genome recoding has been shown to enable nonstandard amino acids, biocontainment and viral resistance in bacteria. Here we take the first steps to extend this to human cells demonstrating exceptional base editing to convert TAG to TAA for 33 essential genes via a single transfection, and examine base-editing genome-wide (observing ~40 C-to-T off-target events in essential gene exons). We also introduce GRIT, a computational tool for recoding. This demonstrates the feasibility of recoding, and highly multiplex editing in mammalian cells.

Suggested Citation

  • Yuting Chen & Eriona Hysolli & Anlu Chen & Stephen Casper & Songlei Liu & Kevin Yang & Chenli Liu & George Church, 2022. "Multiplex base editing to convert TAG into TAA codons in the human genome," Nature Communications, Nature, vol. 13(1), pages 1-13, December.
    • Handle: RePEc:nat:natcom:v:13:y:2022:i:1:d:10.1038_s41467-022-31927-8
    DOI: 10.1038/s41467-022-31927-8
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    References listed on IDEAS

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