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Recoded organisms engineered to depend on synthetic amino acids

Author

Listed:
  • Alexis J. Rovner

    (Cellular and Developmental Biology, Yale University
    Systems Biology Institute, Yale University)

  • Adrian D. Haimovich

    (Cellular and Developmental Biology, Yale University
    Systems Biology Institute, Yale University)

  • Spencer R. Katz

    (Cellular and Developmental Biology, Yale University
    Systems Biology Institute, Yale University)

  • Zhe Li

    (Cellular and Developmental Biology, Yale University
    Systems Biology Institute, Yale University)

  • Michael W. Grome

    (Cellular and Developmental Biology, Yale University
    Systems Biology Institute, Yale University)

  • Brandon M. Gassaway

    (Systems Biology Institute, Yale University
    Yale University)

  • Miriam Amiram

    (Cellular and Developmental Biology, Yale University
    Systems Biology Institute, Yale University)

  • Jaymin R. Patel

    (Cellular and Developmental Biology, Yale University
    Systems Biology Institute, Yale University)

  • Ryan R. Gallagher

    (Cellular and Developmental Biology, Yale University
    Systems Biology Institute, Yale University)

  • Jesse Rinehart

    (Systems Biology Institute, Yale University
    Yale University)

  • Farren J. Isaacs

    (Cellular and Developmental Biology, Yale University
    Systems Biology Institute, Yale University)

Abstract

Construction of a series of genomically recoded organisms whose growth is restricted by the expression of essential genes dependent on exogenously supplied synthetic amino acids introduces novel orthogonal barriers between these engineered organisms and the environment, thereby creating safer genetically modified organisms.

Suggested Citation

  • Alexis J. Rovner & Adrian D. Haimovich & Spencer R. Katz & Zhe Li & Michael W. Grome & Brandon M. Gassaway & Miriam Amiram & Jaymin R. Patel & Ryan R. Gallagher & Jesse Rinehart & Farren J. Isaacs, 2015. "Recoded organisms engineered to depend on synthetic amino acids," Nature, Nature, vol. 518(7537), pages 89-93, February.
    • Handle: RePEc:nat:nature:v:518:y:2015:i:7537:d:10.1038_nature14095
    DOI: 10.1038/nature14095
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    Cited by:

    1. Stefan A. Hoffmann & Yizhi Cai, 2024. "Engineering stringent genetic biocontainment of yeast with a protein stability switch," Nature Communications, Nature, vol. 15(1), pages 1-11, December.
    2. Yuting Chen & Eriona Hysolli & Anlu Chen & Stephen Casper & Songlei Liu & Kevin Yang & Chenli Liu & George Church, 2022. "Multiplex base editing to convert TAG into TAA codons in the human genome," Nature Communications, Nature, vol. 13(1), pages 1-13, December.
    3. Michael Pigula & Yen-Chung Lai & Minseob Koh & Christian S. Diercks & Thomas F. Rogers & David A. Dik & Peter G. Schultz, 2024. "An unnatural amino acid dependent, conditional Pseudomonas vaccine prevents bacterial infection," Nature Communications, Nature, vol. 15(1), pages 1-9, December.
    4. Hongxia Zhao & Wenlong Ding & Jia Zang & Yang Yang & Chao Liu & Linzhen Hu & Yulin Chen & Guanglong Liu & Yu Fang & Ying Yuan & Shixian Lin, 2021. "Directed-evolution of translation system for efficient unnatural amino acids incorporation and generalizable synthetic auxotroph construction," Nature Communications, Nature, vol. 12(1), pages 1-12, December.
    5. Naoki Hayashi & Yong Lai & Jay Fuerte-Stone & Mark Mimee & Timothy K. Lu, 2024. "Cas9-assisted biological containment of a genetically engineered human commensal bacterium and genetic elements," Nature Communications, Nature, vol. 15(1), pages 1-17, December.
    6. Tiantian Chang & Weichao Ding & Shirui Yan & Yun Wang & Haoling Zhang & Yu Zhang & Zhi Ping & Huiming Zhang & Yijian Huang & Jiahui Zhang & Dan Wang & Wenwei Zhang & Xun Xu & Yue Shen & Xian Fu, 2023. "A robust yeast biocontainment system with two-layered regulation switch dependent on unnatural amino acid," Nature Communications, Nature, vol. 14(1), pages 1-13, December.

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