IDEAS home Printed from https://ideas.repec.org/a/nat/nature/v477y2011i7365d10.1038_nature10403.html
   My bibliography  Save this article

Synthetic chromosome arms function in yeast and generate phenotypic diversity by design

Author

Listed:
  • Jessica S. Dymond

    (High Throughput Biology Center, Johns Hopkins University School of Medicine, 733 North Broadway, Baltimore, Maryland 21205, USA
    Johns Hopkins University School of Medicine, 725 North Wolfe Street, Baltimore, Maryland 21205, USA
    Present addresses: Animal Biosciences and Biotechnology Laboratory, Agricultural Research Service, USDA, 10300 Baltimore Avenue, Beltsville, Maryland 20705, USA (J.S.D.) . GreenLight Biosciences, Inc., 196 Boston Avenue, Suite 2400, Medford, Massachusetts 02155, USA (W.J.B.) ; Battelle Memorial Institute, 2987 Clairmont Road NE, Atlanta, Georgia 30329, USA (J.W.S.) ; School of Life Sciences, Tsinghua University, Beijing 100084, China (J.D.) ; Agilent Laboratories, 5301 Stevens Creek Boulevard, Mailstop 53L-IB, Santa Clara, California 95051, USA (D.L.L.); Bowdoin College, 5000 College Station, Brunswick, Maine 04011, USA (A.C.B.).)

  • Sarah M. Richardson

    (High Throughput Biology Center, Johns Hopkins University School of Medicine, 733 North Broadway, Baltimore, Maryland 21205, USA
    McKusick-Nathans Institute of Genetic Medicine, Johns Hopkins University School of Medicine, 733 North Broadway, Baltimore, Maryland 21205, USA)

  • Candice E. Coombes

    (High Throughput Biology Center, Johns Hopkins University School of Medicine, 733 North Broadway, Baltimore, Maryland 21205, USA
    Johns Hopkins University School of Medicine, 725 North Wolfe Street, Baltimore, Maryland 21205, USA)

  • Timothy Babatz

    (High Throughput Biology Center, Johns Hopkins University School of Medicine, 733 North Broadway, Baltimore, Maryland 21205, USA
    McKusick-Nathans Institute of Genetic Medicine, Johns Hopkins University School of Medicine, 733 North Broadway, Baltimore, Maryland 21205, USA)

  • Héloïse Muller

    (Bloomberg School of Public Health, Johns Hopkins University, 615 North Wolfe Street, Baltimore, Maryland 21205, USA)

  • Narayana Annaluru

    (Bloomberg School of Public Health, Johns Hopkins University, 615 North Wolfe Street, Baltimore, Maryland 21205, USA)

  • William J. Blake

    (Codon Devices, 99 Erie Street, Cambridge, Massachusetts 02139, USA
    Present addresses: Animal Biosciences and Biotechnology Laboratory, Agricultural Research Service, USDA, 10300 Baltimore Avenue, Beltsville, Maryland 20705, USA (J.S.D.) . GreenLight Biosciences, Inc., 196 Boston Avenue, Suite 2400, Medford, Massachusetts 02155, USA (W.J.B.) ; Battelle Memorial Institute, 2987 Clairmont Road NE, Atlanta, Georgia 30329, USA (J.W.S.) ; School of Life Sciences, Tsinghua University, Beijing 100084, China (J.D.) ; Agilent Laboratories, 5301 Stevens Creek Boulevard, Mailstop 53L-IB, Santa Clara, California 95051, USA (D.L.L.); Bowdoin College, 5000 College Station, Brunswick, Maine 04011, USA (A.C.B.).)

  • Joy W. Schwerzmann

    (Bloomberg School of Public Health, Johns Hopkins University, 615 North Wolfe Street, Baltimore, Maryland 21205, USA
    Present addresses: Animal Biosciences and Biotechnology Laboratory, Agricultural Research Service, USDA, 10300 Baltimore Avenue, Beltsville, Maryland 20705, USA (J.S.D.) . GreenLight Biosciences, Inc., 196 Boston Avenue, Suite 2400, Medford, Massachusetts 02155, USA (W.J.B.) ; Battelle Memorial Institute, 2987 Clairmont Road NE, Atlanta, Georgia 30329, USA (J.W.S.) ; School of Life Sciences, Tsinghua University, Beijing 100084, China (J.D.) ; Agilent Laboratories, 5301 Stevens Creek Boulevard, Mailstop 53L-IB, Santa Clara, California 95051, USA (D.L.L.); Bowdoin College, 5000 College Station, Brunswick, Maine 04011, USA (A.C.B.).)

  • Junbiao Dai

    (High Throughput Biology Center, Johns Hopkins University School of Medicine, 733 North Broadway, Baltimore, Maryland 21205, USA
    Johns Hopkins University School of Medicine, 725 North Wolfe Street, Baltimore, Maryland 21205, USA
    Present addresses: Animal Biosciences and Biotechnology Laboratory, Agricultural Research Service, USDA, 10300 Baltimore Avenue, Beltsville, Maryland 20705, USA (J.S.D.) . GreenLight Biosciences, Inc., 196 Boston Avenue, Suite 2400, Medford, Massachusetts 02155, USA (W.J.B.) ; Battelle Memorial Institute, 2987 Clairmont Road NE, Atlanta, Georgia 30329, USA (J.W.S.) ; School of Life Sciences, Tsinghua University, Beijing 100084, China (J.D.) ; Agilent Laboratories, 5301 Stevens Creek Boulevard, Mailstop 53L-IB, Santa Clara, California 95051, USA (D.L.L.); Bowdoin College, 5000 College Station, Brunswick, Maine 04011, USA (A.C.B.).)

  • Derek L. Lindstrom

    (Fred Hutchinson Cancer Research Center, Mailstop A3-025, PO Box 19024, Seattle, Washington 98109, USA
    Present addresses: Animal Biosciences and Biotechnology Laboratory, Agricultural Research Service, USDA, 10300 Baltimore Avenue, Beltsville, Maryland 20705, USA (J.S.D.) . GreenLight Biosciences, Inc., 196 Boston Avenue, Suite 2400, Medford, Massachusetts 02155, USA (W.J.B.) ; Battelle Memorial Institute, 2987 Clairmont Road NE, Atlanta, Georgia 30329, USA (J.W.S.) ; School of Life Sciences, Tsinghua University, Beijing 100084, China (J.D.) ; Agilent Laboratories, 5301 Stevens Creek Boulevard, Mailstop 53L-IB, Santa Clara, California 95051, USA (D.L.L.); Bowdoin College, 5000 College Station, Brunswick, Maine 04011, USA (A.C.B.).)

  • Annabel C. Boeke

    (High Throughput Biology Center, Johns Hopkins University School of Medicine, 733 North Broadway, Baltimore, Maryland 21205, USA
    Present addresses: Animal Biosciences and Biotechnology Laboratory, Agricultural Research Service, USDA, 10300 Baltimore Avenue, Beltsville, Maryland 20705, USA (J.S.D.) . GreenLight Biosciences, Inc., 196 Boston Avenue, Suite 2400, Medford, Massachusetts 02155, USA (W.J.B.) ; Battelle Memorial Institute, 2987 Clairmont Road NE, Atlanta, Georgia 30329, USA (J.W.S.) ; School of Life Sciences, Tsinghua University, Beijing 100084, China (J.D.) ; Agilent Laboratories, 5301 Stevens Creek Boulevard, Mailstop 53L-IB, Santa Clara, California 95051, USA (D.L.L.); Bowdoin College, 5000 College Station, Brunswick, Maine 04011, USA (A.C.B.).)

  • Daniel E. Gottschling

    (Fred Hutchinson Cancer Research Center, Mailstop A3-025, PO Box 19024, Seattle, Washington 98109, USA)

  • Srinivasan Chandrasegaran

    (Bloomberg School of Public Health, Johns Hopkins University, 615 North Wolfe Street, Baltimore, Maryland 21205, USA)

  • Joel S. Bader

    (High Throughput Biology Center, Johns Hopkins University School of Medicine, 733 North Broadway, Baltimore, Maryland 21205, USA
    Whiting School of Engineering, Johns Hopkins University, 3400 North Charles Street, Baltimore, Maryland 21218, USA)

  • Jef D. Boeke

    (High Throughput Biology Center, Johns Hopkins University School of Medicine, 733 North Broadway, Baltimore, Maryland 21205, USA
    Johns Hopkins University School of Medicine, 725 North Wolfe Street, Baltimore, Maryland 21205, USA)

Abstract

A part-synthetic yeast genome A milestone in biology was reached in 2010, with the production of a viable bacterium with a genome that had been reassembled artificially from synthetic DNA segments. Now Jef Boeke and colleagues report the production of the world's first synthetic eukaryotic chromosome arms, a first step in a project called Sc2.0, which aims to design and construct an entirely synthetic version of the Saccharomyces cerevisiae genome. In this initial phase, two custom-designed synthetic chromosome arms were incorporated into the yeast genome, replacing the endogenous sequence.

Suggested Citation

  • Jessica S. Dymond & Sarah M. Richardson & Candice E. Coombes & Timothy Babatz & Héloïse Muller & Narayana Annaluru & William J. Blake & Joy W. Schwerzmann & Junbiao Dai & Derek L. Lindstrom & Annabel , 2011. "Synthetic chromosome arms function in yeast and generate phenotypic diversity by design," Nature, Nature, vol. 477(7365), pages 471-476, September.
    • Handle: RePEc:nat:nature:v:477:y:2011:i:7365:d:10.1038_nature10403
    DOI: 10.1038/nature10403
    as

    Download full text from publisher

    File URL: https://www.nature.com/articles/nature10403
    File Function: Abstract
    Download Restriction: Access to the full text of the articles in this series is restricted.
    File URL: https://libkey.io/10.1038/nature10403?utm_source=ideas
    LibKey link: if access is restricted and if your library uses this service, LibKey will redirect you to where you can use your library subscription to access this item
    ---><---

    As the access to this document is restricted, you may want to search for a different version of it.

    Citations

    Citations are extracted by the CitEc Project, subscribe to its RSS feed for this item.
    as


    Cited by:

    1. Dariusz R. Kutyna & Cristobal A. Onetto & Thomas C. Williams & Hugh D. Goold & Ian T. Paulsen & Isak S. Pretorius & Daniel L. Johnson & Anthony R. Borneman, 2022. "Construction of a synthetic Saccharomyces cerevisiae pan-genome neo-chromosome," Nature Communications, Nature, vol. 13(1), pages 1-9, December.
    2. Charlotte Cautereels & Jolien Smets & Peter Bircham & Dries De Ruysscher & Anna Zimmermann & Peter De Rijk & Jan Steensels & Anton Gorkovskiy & Joleen Masschelein & Kevin J. Verstrepen, 2024. "Combinatorial optimization of gene expression through recombinase-mediated promoter and terminator shuffling in yeast," Nature Communications, Nature, vol. 15(1), pages 1-17, December.
    3. Yuting Chen & Eriona Hysolli & Anlu Chen & Stephen Casper & Songlei Liu & Kevin Yang & Chenli Liu & George Church, 2022. "Multiplex base editing to convert TAG into TAA codons in the human genome," Nature Communications, Nature, vol. 13(1), pages 1-13, December.
    4. Hideto Mori & Nozomu Yachie, 2022. "A framework to efficiently describe and share reproducible DNA materials and construction protocols," Nature Communications, Nature, vol. 13(1), pages 1-9, December.
    5. Huiming Zhang & Xian Fu & Xuemei Gong & Yun Wang & Haolin Zhang & Yu Zhao & Yue Shen, 2022. "Systematic dissection of key factors governing recombination outcomes by GCE-SCRaMbLE," Nature Communications, Nature, vol. 13(1), pages 1-11, December.
    6. Shuangying Jiang & Zhouqing Luo & Jie Wu & Kang Yu & Shijun Zhao & Zelin Cai & Wenfei Yu & Hui Wang & Li Cheng & Zhenzhen Liang & Hui Gao & Marco Monti & Daniel Schindler & Linsen Huang & Cheng Zeng &, 2023. "Building a eukaryotic chromosome arm by de novo design and synthesis," Nature Communications, Nature, vol. 14(1), pages 1-17, December.
    7. Li Cheng & Shijun Zhao & Tianyi Li & Sha Hou & Zhouqing Luo & Jinsheng Xu & Wenfei Yu & Shuangying Jiang & Marco Monti & Daniel Schindler & Weimin Zhang & Chunhui Hou & Yingxin Ma & Yizhi Cai & Jef D., 2024. "Large-scale genomic rearrangements boost SCRaMbLE in Saccharomyces cerevisiae," Nature Communications, Nature, vol. 15(1), pages 1-15, December.
    8. Alessandro L. V. Coradini & Christopher Ne Ville & Zachary A. Krieger & Joshua Roemer & Cara Hull & Shawn Yang & Daniel T. Lusk & Ian M. Ehrenreich, 2023. "Building synthetic chromosomes from natural DNA," Nature Communications, Nature, vol. 14(1), pages 1-12, December.
    9. Tiantian Chang & Weichao Ding & Shirui Yan & Yun Wang & Haoling Zhang & Yu Zhang & Zhi Ping & Huiming Zhang & Yijian Huang & Jiahui Zhang & Dan Wang & Wenwei Zhang & Xun Xu & Yue Shen & Xian Fu, 2023. "A robust yeast biocontainment system with two-layered regulation switch dependent on unnatural amino acid," Nature Communications, Nature, vol. 14(1), pages 1-13, December.

    More about this item

    Statistics

    Access and download statistics

    Corrections

    All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:nat:nature:v:477:y:2011:i:7365:d:10.1038_nature10403. See general information about how to correct material in RePEc.

    If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.

    We have no bibliographic references for this item. You can help adding them by using this form .

    If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.

    For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: Sonal Shukla or Springer Nature Abstracting and Indexing (email available below). General contact details of provider: http://www.nature.com .

    Please note that corrections may take a couple of weeks to filter through the various RePEc services.

    IDEAS is a RePEc service. RePEc uses bibliographic data supplied by the respective publishers.