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Functional and multiscale 3D structural investigation of brain tissue through correlative in vivo physiology, synchrotron microtomography and volume electron microscopy

Author

Listed:
  • Carles Bosch

    (The Francis Crick Institute)

  • Tobias Ackels

    (The Francis Crick Institute
    University College)

  • Alexandra Pacureanu

    (The Francis Crick Institute
    University College
    ESRF, The European Synchrotron)

  • Yuxin Zhang

    (The Francis Crick Institute
    University College)

  • Christopher J. Peddie

    (The Francis Crick Institute)

  • Manuel Berning

    (Max Planck Institute for Brain Research
    Scalable minds GmbH)

  • Norman Rzepka

    (Scalable minds GmbH)

  • Marie-Christine Zdora

    (University College London
    Diamond Light Source, Harwell Science and Innovation Campus
    University of Southampton)

  • Isabell Whiteley

    (The Francis Crick Institute
    University College)

  • Malte Storm

    (Diamond Light Source, Harwell Science and Innovation Campus
    Helmholtz-Zentrum Hereon)

  • Anne Bonnin

    (Paul Scherrer Institut)

  • Christoph Rau

    (Diamond Light Source, Harwell Science and Innovation Campus)

  • Troy Margrie

    (University College London)

  • Lucy Collinson

    (The Francis Crick Institute)

  • Andreas T. Schaefer

    (The Francis Crick Institute
    University College)

Abstract

Understanding the function of biological tissues requires a coordinated study of physiology and structure, exploring volumes that contain complete functional units at a detail that resolves the relevant features. Here, we introduce an approach to address this challenge: Mouse brain tissue sections containing a region where function was recorded using in vivo 2-photon calcium imaging were stained, dehydrated, resin-embedded and imaged with synchrotron X-ray computed tomography with propagation-based phase contrast (SXRT). SXRT provided context at subcellular detail, and could be followed by targeted acquisition of multiple volumes using serial block-face electron microscopy (SBEM). In the olfactory bulb, combining SXRT and SBEM enabled disambiguation of in vivo-assigned regions of interest. In the hippocampus, we found that superficial pyramidal neurons in CA1a displayed a larger density of spine apparati than deeper ones. Altogether, this approach can enable a functional and structural investigation of subcellular features in the context of cells and tissues.

Suggested Citation

  • Carles Bosch & Tobias Ackels & Alexandra Pacureanu & Yuxin Zhang & Christopher J. Peddie & Manuel Berning & Norman Rzepka & Marie-Christine Zdora & Isabell Whiteley & Malte Storm & Anne Bonnin & Chris, 2022. "Functional and multiscale 3D structural investigation of brain tissue through correlative in vivo physiology, synchrotron microtomography and volume electron microscopy," Nature Communications, Nature, vol. 13(1), pages 1-16, December.
    • Handle: RePEc:nat:natcom:v:13:y:2022:i:1:d:10.1038_s41467-022-30199-6
    DOI: 10.1038/s41467-022-30199-6
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    References listed on IDEAS

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