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Architecture of a mammalian glomerular domain revealed by novel volume electroporation using nanoengineered microelectrodes

Author

Listed:
  • D. Schwarz

    (Max Planck Institute for Medical Research
    Heidelberg University Hospital
    University of Heidelberg)

  • M. Kollo

    (Max Planck Institute for Medical Research
    The Francis Crick Institute
    University College London)

  • C. Bosch

    (The Francis Crick Institute
    University College London)

  • C. Feinauer

    (Max Planck Institute for Medical Research
    University of Heidelberg)

  • I. Whiteley

    (The Francis Crick Institute
    University College London)

  • T. W. Margrie

    (University College London)

  • T. Cutforth

    (Columbia University Medical Center)

  • A. T. Schaefer

    (Max Planck Institute for Medical Research
    University of Heidelberg
    The Francis Crick Institute
    University College London)

Abstract

Dense microcircuit reconstruction techniques have begun to provide ultrafine insight into the architecture of small-scale networks. However, identifying the totality of cells belonging to such neuronal modules, the “inputs” and “outputs,” remains a major challenge. Here, we present the development of nanoengineered electroporation microelectrodes (NEMs) for comprehensive manipulation of a substantial volume of neuronal tissue. Combining finite element modeling and focused ion beam milling, NEMs permit substantially higher stimulation intensities compared to conventional glass capillaries, allowing for larger volumes configurable to the geometry of the target circuit. We apply NEMs to achieve near-complete labeling of the neuronal network associated with a genetically identified olfactory glomerulus. This allows us to detect sparse higher-order features of the wiring architecture that are inaccessible to statistical labeling approaches. Thus, NEM labeling provides crucial complementary information to dense circuit reconstruction techniques. Relying solely on targeting an electrode to the region of interest and passive biophysical properties largely common across cell types, this can easily be employed anywhere in the CNS.

Suggested Citation

  • D. Schwarz & M. Kollo & C. Bosch & C. Feinauer & I. Whiteley & T. W. Margrie & T. Cutforth & A. T. Schaefer, 2018. "Architecture of a mammalian glomerular domain revealed by novel volume electroporation using nanoengineered microelectrodes," Nature Communications, Nature, vol. 9(1), pages 1-14, December.
  • Handle: RePEc:nat:natcom:v:9:y:2018:i:1:d:10.1038_s41467-017-02560-7
    DOI: 10.1038/s41467-017-02560-7
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    Cited by:

    1. Carles Bosch & Tobias Ackels & Alexandra Pacureanu & Yuxin Zhang & Christopher J. Peddie & Manuel Berning & Norman Rzepka & Marie-Christine Zdora & Isabell Whiteley & Malte Storm & Anne Bonnin & Chris, 2022. "Functional and multiscale 3D structural investigation of brain tissue through correlative in vivo physiology, synchrotron microtomography and volume electron microscopy," Nature Communications, Nature, vol. 13(1), pages 1-16, December.
    2. Thomas Carraro & Simon Dörsam & Stefan Frei & Daniel Schwarz, 2018. "An Adaptive Newton Algorithm for Optimal Control Problems with Application to Optimal Electrode Design," Journal of Optimization Theory and Applications, Springer, vol. 177(2), pages 498-534, May.

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