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Fast imaging of millimeter-scale areas with beam deflection transmission electron microscopy

Author

Listed:
  • Zhihao Zheng

    (Princeton University)

  • Christopher S. Own

    (Voxa)

  • Adrian A. Wanner

    (Princeton University
    Paul Scherrer Institute)

  • Randal A. Koene

    (Voxa)

  • Eric W. Hammerschmith

    (Princeton University)

  • William M. Silversmith

    (Princeton University)

  • Nico Kemnitz

    (Princeton University)

  • Ran Lu

    (Princeton University)

  • David W. Tank

    (Princeton University)

  • H. Sebastian Seung

    (Princeton University)

Abstract

Serial section transmission electron microscopy (TEM) has proven to be one of the leading methods for millimeter-scale 3D imaging of brain tissues at nanoscale resolution. It is important to further improve imaging efficiency to acquire larger and more brain volumes. We report here a threefold increase in the speed of TEM by using a beam deflecting mechanism to enable highly efficient acquisition of multiple image tiles (nine) for each motion of the mechanical stage. For millimeter-scale areas, the duty cycle of imaging doubles to more than 30%, yielding a net average imaging rate of 0.3 gigapixels per second. If fully utilized, an array of four beam deflection TEMs should be capable of imaging a dataset of cubic millimeter scale in five weeks.

Suggested Citation

  • Zhihao Zheng & Christopher S. Own & Adrian A. Wanner & Randal A. Koene & Eric W. Hammerschmith & William M. Silversmith & Nico Kemnitz & Ran Lu & David W. Tank & H. Sebastian Seung, 2024. "Fast imaging of millimeter-scale areas with beam deflection transmission electron microscopy," Nature Communications, Nature, vol. 15(1), pages 1-11, December.
    • Handle: RePEc:nat:natcom:v:15:y:2024:i:1:d:10.1038_s41467-024-50846-4
    DOI: 10.1038/s41467-024-50846-4
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