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Development of cellulosic ethanol production process via co-culturing of artificial cellulosomal Bacillus and kefir yeast

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  • Ho, Cheng-Yu
  • Chang, Jui-Jen
  • Lee, Shih-Chi
  • Chin, Tsu-Yuan
  • Shih, Ming-Che
  • Li, Wen-Hsiung
  • Huang, Chieh-Chen

Abstract

A novel dual-microbe Bacillus/yeast co-culture system is developed for cellulosic bioethanol production. A recombinant cellulosomal Bacillus subtilis that carries eight cellulosomal genes of Clostridium thermocellum, including one scaffolding protein gene (cipA), one cell-surface anchor gene (sdbA), two exo-glucosidase genes (celK and celS), two endoglucanase genes (celA and celR), and two xylanase genes (xynC and xynZ) was constructed. The partner microbes for the dual-microbe combination are the wild type kefir yeast Kluyveromyces marxianus KY3, K. marxianus KY3-NpaBGS, which carries a β-glucosidase (NpaBGS) gene from rumen fungus, and the K. marxianus KR5 strain, that harbors endoglucanase (egIII), exo-glucanase (cbhI) and NpaBGS genes. All three Bacillus/yeast co-culture systems could achieve the cellulose saccharification and ethanol conversion simultaneously better than KR5 alone. The combination of Bacillus/KY3-NpaBGS outperformed that of Bacillus/KY3, as they could produce β-glucosidase enzyme for the system. Although, KR5 produces two more kinds of cellulases than KY3-NpaBGS. Bacillus/KR5 could not perform better than Bacillus/KY3-NpaBGS. Our results suggest that the dual-microbe Bacillus/yeast co-culturing system could leverage the advantages from both microbes and have a great potential for integrating into consolidated bioprocessing system.

Suggested Citation

  • Ho, Cheng-Yu & Chang, Jui-Jen & Lee, Shih-Chi & Chin, Tsu-Yuan & Shih, Ming-Che & Li, Wen-Hsiung & Huang, Chieh-Chen, 2012. "Development of cellulosic ethanol production process via co-culturing of artificial cellulosomal Bacillus and kefir yeast," Applied Energy, Elsevier, vol. 100(C), pages 27-32.
    • Handle: RePEc:eee:appene:v:100:y:2012:i:c:p:27-32
    DOI: 10.1016/j.apenergy.2012.03.016
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    2. Raele, Ricardo & Boaventura, João Mauricio Gama & Fischmann, Adalberto Américo & Sarturi, Greici, 2014. "Scenarios for the second generation ethanol in Brazil," Technological Forecasting and Social Change, Elsevier, vol. 87(C), pages 205-223.
    3. Chang, Jui-Jen & Ho, Cheng-Yu & Mao, Chi-Tang & Barham, Nathan & Huang, Yu-Rong & Ho, Feng-Ju & Wu, Yueh-Chin & Hou, Yu-Han & Shih, Ming-Che & Li, Wen-Hsiung & Huang, Chieh-Chen, 2014. "A thermo- and toxin-tolerant kefir yeast for biorefinery and biofuel production," Applied Energy, Elsevier, vol. 132(C), pages 465-474.
    4. Schneider, Willian Daniel Hahn & Fontana, Roselei Claudete & Baudel, Henrique Macedo & de Siqueira, Félix Gonçalves & Rencoret, Jorge & Gutiérrez, Ana & de Eugenio, Laura Isabel & Prieto, Alicia & Mar, 2020. "Lignin degradation and detoxification of eucalyptus wastes by on-site manufacturing fungal enzymes to enhance second-generation ethanol yield," Applied Energy, Elsevier, vol. 262(C).
    5. Singh, Shuchi & Khanna, Swati & Moholkar, Vijayanand S. & Goyal, Arun, 2014. "Screening and optimization of pretreatments for Parthenium hysterophorus as feedstock for alcoholic biofuels," Applied Energy, Elsevier, vol. 129(C), pages 195-206.

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