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Cellulosic ethanol production performance with SSF and SHF processes using immobilized Zymomonas mobilis

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  • Wirawan, Ferdian
  • Cheng, Chieh-Lun
  • Kao, Wei-Chen
  • Lee, Duu-Jong
  • Chang, Jo-Shu

Abstract

Bioethanol converted from lignocellulosic feedstock, such as agricultural waste, is considered one of the most promising biofuels being developed. The raw materials usually need to be pretreated and hydrolyzed by cellulolytic enzymes to produce sugars for subsequent ethanol fermentation. Immobilized bacteria or yeasts have been frequently used for batch or continuous ethanol fermentation due to their feasibility for repeated use with high biomass retention during the process. In this study, Zymomonas mobilis cells immobilized in calcium alginate (CA) and polyvinyl alcohol (PVA) were used to produce ethanol from cellulosic feedstock using SSF (simultaneous saccharification and fermentation) and SHF (separate hydrolysis and fermentation) processes. The performance based on different immobilized cells and different processes was compared. The results show that PVA immobilized cells with the SHF process gave the highest ethanol concentration of 6.24g/L, with an ethanol yield of 79.09% and a maximum ethanol productivity of 3.04g/L/h. In contrast, the performance of CA-immobilized cells with SHF was poorer, with the highest ethanol concentration, ethanol yield, and maximum ethanol productivity of 5.52g/L, 69.96% and 2.37g/L/h, respectively. For the SSF process, the maximum ethanol concentration, ethanol yield, and maximum ethanol productivity were 5.53g/L, 70.09%, and of 1.31g/L/h, respectively, for the PVA immobilized Z. mobilis, while they were 5.44g/L, 68.95%, and 1.27g/L/h for CA immobilized cells. The comparison with suspended cells shows that the immobilized cells of Z. mobilis are feasible for ethanol production via SSF and SHF.

Suggested Citation

  • Wirawan, Ferdian & Cheng, Chieh-Lun & Kao, Wei-Chen & Lee, Duu-Jong & Chang, Jo-Shu, 2012. "Cellulosic ethanol production performance with SSF and SHF processes using immobilized Zymomonas mobilis," Applied Energy, Elsevier, vol. 100(C), pages 19-26.
    • Handle: RePEc:eee:appene:v:100:y:2012:i:c:p:19-26
    DOI: 10.1016/j.apenergy.2012.04.032
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    1. Monte, J.R. & Brienzo, M. & Milagres, A.M.F., 2011. "Utilization of pineapple stem juice to enhance enzyme-hydrolytic efficiency for sugarcane bagasse after an optimized pre-treatment with alkaline peroxide," Applied Energy, Elsevier, vol. 88(1), pages 403-408, January.
    2. Zheng, Yi & Yu, Chaowei & Cheng, Yu-Shen & Lee, Christopher & Simmons, Christopher W. & Dooley, Todd M. & Zhang, Ruihong & Jenkins, Bryan M. & VanderGheynst, Jean S., 2012. "Integrating sugar beet pulp storage, hydrolysis and fermentation for fuel ethanol production," Applied Energy, Elsevier, vol. 93(C), pages 168-175.
    3. Li, Shi-Zhong & Chan-Halbrendt, Catherine, 2009. "Ethanol production in (the) People's Republic of China: Potential and technologies," Applied Energy, Elsevier, vol. 86(Supplemen), pages 162-169, November.
    4. Behera, Shuvashish & Mohanty, Rama Chandra & Ray, Ramesh Chandra, 2010. "Comparative study of bio-ethanol production from mahula (Madhuca latifolia L.) flowers by Saccharomyces cerevisiae and Zymomonas mobilis," Applied Energy, Elsevier, vol. 87(7), pages 2352-2355, July.
    5. Zheng, Yi & Pan, Zhongli & Zhang, Ruihong & Wang, Donghai, 2009. "Enzymatic saccharification of dilute acid pretreated saline crops for fermentable sugar production," Applied Energy, Elsevier, vol. 86(11), pages 2459-2465, November.
    6. Starfelt, Fredrik & Daianova, Lilia & Yan, Jinyue & Thorin, Eva & Dotzauer, Erik, 2012. "The impact of lignocellulosic ethanol yields in polygeneration with district heating – A case study," Applied Energy, Elsevier, vol. 92(C), pages 791-799.
    7. Eriksson, Gunnar & Kjellström, Björn, 2010. "Assessment of combined heat and power (CHP) integrated with wood-based ethanol production," Applied Energy, Elsevier, vol. 87(12), pages 3632-3641, December.
    8. Behera, Shuvashish & Kar, Shaktimay & Mohanty, Rama Chandra & Ray, Ramesh Chandra, 2010. "Comparative study of bio-ethanol production from mahula (Madhuca latifolia L.) flowers by Saccharomyces cerevisiae cells immobilized in agar agar and Ca-alginate matrices," Applied Energy, Elsevier, vol. 87(1), pages 96-100, January.
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    2. Lin, Yu-Sheng & Lee, Wen-Chien & Duan, Kow-Jen & Lin, Yen-Han, 2013. "Ethanol production by simultaneous saccharification and fermentation in rotary drum reactor using thermotolerant Kluveromyces marxianus," Applied Energy, Elsevier, vol. 105(C), pages 389-394.
    3. Wang, Pixiang & Chen, Yong Mei & Wang, Yifen & Lee, Yoon Y. & Zong, Wenming & Taylor, Steven & McDonald, Timothy & Wang, Yi, 2019. "Towards comprehensive lignocellulosic biomass utilization for bioenergy production: Efficient biobutanol production from acetic acid pretreated switchgrass with Clostridium saccharoperbutylacetonicum ," Applied Energy, Elsevier, vol. 236(C), pages 551-559.
    4. Wirawan, Ferdian & Cheng, Chieh-Lun & Lo, Yung-Chung & Chen, Chun-Yen & Chang, Jo-Shu & Leu, Shao-Yuan & Lee, Duu-Jong, 2020. "Continuous cellulosic bioethanol co-fermentation by immobilized Zymomonas mobilis and suspended Pichia stipitis in a two-stage process," Applied Energy, Elsevier, vol. 266(C).
    5. Karagoz, Pınar & Bill, Roslyn M. & Ozkan, Melek, 2019. "Lignocellulosic ethanol production: Evaluation of new approaches, cell immobilization and reactor configurations," Renewable Energy, Elsevier, vol. 143(C), pages 741-752.
    6. Cheah, Wai Yan & Ling, Tau Chuan & Show, Pau Loke & Juan, Joon Ching & Chang, Jo-Shu & Lee, Duu-Jong, 2016. "Cultivation in wastewaters for energy: A microalgae platform," Applied Energy, Elsevier, vol. 179(C), pages 609-625.
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    8. Kuo, Yen-Ting & Chen, Ju-Shiou & Yang, Tzu-Yueh & Wan, Hou-Peng, 2018. "Technical and Economic approach of bioethanol production from nanofiltration of biomass chemical hydrolysis solutions," Applied Energy, Elsevier, vol. 215(C), pages 426-436.

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