IDEAS home Printed from https://ideas.repec.org/a/eee/appene/v100y2012icp19-26.html
   My bibliography  Save this article

Cellulosic ethanol production performance with SSF and SHF processes using immobilized Zymomonas mobilis

Author

Listed:
  • Wirawan, Ferdian
  • Cheng, Chieh-Lun
  • Kao, Wei-Chen
  • Lee, Duu-Jong
  • Chang, Jo-Shu

Abstract

Bioethanol converted from lignocellulosic feedstock, such as agricultural waste, is considered one of the most promising biofuels being developed. The raw materials usually need to be pretreated and hydrolyzed by cellulolytic enzymes to produce sugars for subsequent ethanol fermentation. Immobilized bacteria or yeasts have been frequently used for batch or continuous ethanol fermentation due to their feasibility for repeated use with high biomass retention during the process. In this study, Zymomonas mobilis cells immobilized in calcium alginate (CA) and polyvinyl alcohol (PVA) were used to produce ethanol from cellulosic feedstock using SSF (simultaneous saccharification and fermentation) and SHF (separate hydrolysis and fermentation) processes. The performance based on different immobilized cells and different processes was compared. The results show that PVA immobilized cells with the SHF process gave the highest ethanol concentration of 6.24g/L, with an ethanol yield of 79.09% and a maximum ethanol productivity of 3.04g/L/h. In contrast, the performance of CA-immobilized cells with SHF was poorer, with the highest ethanol concentration, ethanol yield, and maximum ethanol productivity of 5.52g/L, 69.96% and 2.37g/L/h, respectively. For the SSF process, the maximum ethanol concentration, ethanol yield, and maximum ethanol productivity were 5.53g/L, 70.09%, and of 1.31g/L/h, respectively, for the PVA immobilized Z. mobilis, while they were 5.44g/L, 68.95%, and 1.27g/L/h for CA immobilized cells. The comparison with suspended cells shows that the immobilized cells of Z. mobilis are feasible for ethanol production via SSF and SHF.

Suggested Citation

  • Wirawan, Ferdian & Cheng, Chieh-Lun & Kao, Wei-Chen & Lee, Duu-Jong & Chang, Jo-Shu, 2012. "Cellulosic ethanol production performance with SSF and SHF processes using immobilized Zymomonas mobilis," Applied Energy, Elsevier, vol. 100(C), pages 19-26.
    • Handle: RePEc:eee:appene:v:100:y:2012:i:c:p:19-26
    DOI: 10.1016/j.apenergy.2012.04.032
    as

    Download full text from publisher

    File URL: http://www.sciencedirect.com/science/article/pii/S0306261912003182
    Download Restriction: Full text for ScienceDirect subscribers only
    File URL: https://libkey.io/10.1016/j.apenergy.2012.04.032?utm_source=ideas
    LibKey link: if access is restricted and if your library uses this service, LibKey will redirect you to where you can use your library subscription to access this item
    ---><---

    As the access to this document is restricted, you may want to search for a different version of it.

    References listed on IDEAS

    as
    1. Behera, Shuvashish & Mohanty, Rama Chandra & Ray, Ramesh Chandra, 2010. "Comparative study of bio-ethanol production from mahula (Madhuca latifolia L.) flowers by Saccharomyces cerevisiae and Zymomonas mobilis," Applied Energy, Elsevier, vol. 87(7), pages 2352-2355, July.
    2. Behera, Shuvashish & Kar, Shaktimay & Mohanty, Rama Chandra & Ray, Ramesh Chandra, 2010. "Comparative study of bio-ethanol production from mahula (Madhuca latifolia L.) flowers by Saccharomyces cerevisiae cells immobilized in agar agar and Ca-alginate matrices," Applied Energy, Elsevier, vol. 87(1), pages 96-100, January.
    3. Zheng, Yi & Yu, Chaowei & Cheng, Yu-Shen & Lee, Christopher & Simmons, Christopher W. & Dooley, Todd M. & Zhang, Ruihong & Jenkins, Bryan M. & VanderGheynst, Jean S., 2012. "Integrating sugar beet pulp storage, hydrolysis and fermentation for fuel ethanol production," Applied Energy, Elsevier, vol. 93(C), pages 168-175.
    4. Monte, J.R. & Brienzo, M. & Milagres, A.M.F., 2011. "Utilization of pineapple stem juice to enhance enzyme-hydrolytic efficiency for sugarcane bagasse after an optimized pre-treatment with alkaline peroxide," Applied Energy, Elsevier, vol. 88(1), pages 403-408, January.
    5. Li, Shi-Zhong & Chan-Halbrendt, Catherine, 2009. "Ethanol production in (the) People's Republic of China: Potential and technologies," Applied Energy, Elsevier, vol. 86(Supplemen), pages 162-169, November.
    6. Zheng, Yi & Pan, Zhongli & Zhang, Ruihong & Wang, Donghai, 2009. "Enzymatic saccharification of dilute acid pretreated saline crops for fermentable sugar production," Applied Energy, Elsevier, vol. 86(11), pages 2459-2465, November.
    7. Starfelt, Fredrik & Daianova, Lilia & Yan, Jinyue & Thorin, Eva & Dotzauer, Erik, 2012. "The impact of lignocellulosic ethanol yields in polygeneration with district heating – A case study," Applied Energy, Elsevier, vol. 92(C), pages 791-799.
    8. Eriksson, Gunnar & Kjellström, Björn, 2010. "Assessment of combined heat and power (CHP) integrated with wood-based ethanol production," Applied Energy, Elsevier, vol. 87(12), pages 3632-3641, December.
    Full references (including those not matched with items on IDEAS)

    Citations

    Citations are extracted by the CitEc Project, subscribe to its RSS feed for this item.
    as


    Cited by:

    1. Wirawan, Ferdian & Cheng, Chieh-Lun & Lo, Yung-Chung & Chen, Chun-Yen & Chang, Jo-Shu & Leu, Shao-Yuan & Lee, Duu-Jong, 2020. "Continuous cellulosic bioethanol co-fermentation by immobilized Zymomonas mobilis and suspended Pichia stipitis in a two-stage process," Applied Energy, Elsevier, vol. 266(C).
    2. Karagoz, Pınar & Bill, Roslyn M. & Ozkan, Melek, 2019. "Lignocellulosic ethanol production: Evaluation of new approaches, cell immobilization and reactor configurations," Renewable Energy, Elsevier, vol. 143(C), pages 741-752.
    3. Gianluca Cavalaglio & Mattia Gelosia & Silvia D’Antonio & Andrea Nicolini & Anna Laura Pisello & Marco Barbanera & Franco Cotana, 2016. "Lignocellulosic Ethanol Production from the Recovery of Stranded Driftwood Residues," Energies, MDPI, vol. 9(8), pages 1-10, August.
    4. Lin, Yu-Sheng & Lee, Wen-Chien & Duan, Kow-Jen & Lin, Yen-Han, 2013. "Ethanol production by simultaneous saccharification and fermentation in rotary drum reactor using thermotolerant Kluveromyces marxianus," Applied Energy, Elsevier, vol. 105(C), pages 389-394.
    5. Wang, Pixiang & Chen, Yong Mei & Wang, Yifen & Lee, Yoon Y. & Zong, Wenming & Taylor, Steven & McDonald, Timothy & Wang, Yi, 2019. "Towards comprehensive lignocellulosic biomass utilization for bioenergy production: Efficient biobutanol production from acetic acid pretreated switchgrass with Clostridium saccharoperbutylacetonicum ," Applied Energy, Elsevier, vol. 236(C), pages 551-559.
    6. Cheah, Wai Yan & Ling, Tau Chuan & Show, Pau Loke & Juan, Joon Ching & Chang, Jo-Shu & Lee, Duu-Jong, 2016. "Cultivation in wastewaters for energy: A microalgae platform," Applied Energy, Elsevier, vol. 179(C), pages 609-625.
    7. Ntihuga, Jean Nepomuscene & Senn, Thomas & Gschwind, Peter & Kohlus, Reinhard, 2013. "An evaluation of different bioreactor configurations for continuous bio-ethanol production," Applied Energy, Elsevier, vol. 108(C), pages 194-201.
    8. Kuo, Yen-Ting & Chen, Ju-Shiou & Yang, Tzu-Yueh & Wan, Hou-Peng, 2018. "Technical and Economic approach of bioethanol production from nanofiltration of biomass chemical hydrolysis solutions," Applied Energy, Elsevier, vol. 215(C), pages 426-436.

    Most related items

    These are the items that most often cite the same works as this one and are cited by the same works as this one.
    1. Wirawan, Ferdian & Cheng, Chieh-Lun & Lo, Yung-Chung & Chen, Chun-Yen & Chang, Jo-Shu & Leu, Shao-Yuan & Lee, Duu-Jong, 2020. "Continuous cellulosic bioethanol co-fermentation by immobilized Zymomonas mobilis and suspended Pichia stipitis in a two-stage process," Applied Energy, Elsevier, vol. 266(C).
    2. Ho, Cheng-Yu & Chang, Jui-Jen & Lee, Shih-Chi & Chin, Tsu-Yuan & Shih, Ming-Che & Li, Wen-Hsiung & Huang, Chieh-Chen, 2012. "Development of cellulosic ethanol production process via co-culturing of artificial cellulosomal Bacillus and kefir yeast," Applied Energy, Elsevier, vol. 100(C), pages 27-32.
    3. Dodić, Jelena M. & Vučurović, Damjan G. & Dodić, Siniša N. & Grahovac, Jovana A. & Popov, Stevan D. & Nedeljković, Nataša M., 2012. "Kinetic modelling of batch ethanol production from sugar beet raw juice," Applied Energy, Elsevier, vol. 99(C), pages 192-197.
    4. Lou, Rui & Wu, Shu-bin, 2011. "Products properties from fast pyrolysis of enzymatic/mild acidolysis lignin," Applied Energy, Elsevier, vol. 88(1), pages 316-322, January.
    5. Daraei, Mahsa & Avelin, Anders & Dotzauer, Erik & Thorin, Eva, 2019. "Evaluation of biofuel production integrated with existing CHP plants and the impacts on production planning of the system – A case study," Applied Energy, Elsevier, vol. 252(C), pages 1-1.
    6. Ntihuga, Jean Nepomuscene & Senn, Thomas & Gschwind, Peter & Kohlus, Reinhard, 2013. "An evaluation of different bioreactor configurations for continuous bio-ethanol production," Applied Energy, Elsevier, vol. 108(C), pages 194-201.
    7. Borujeni, Nasim Espah & Karimi, Keikhosro & Denayer, Joeri F.M. & Kumar, Rajeev, 2022. "Apple pomace biorefinery for ethanol, mycoprotein, and value-added biochemicals production by Mucor indicus," Energy, Elsevier, vol. 240(C).
    8. Tripti Agrawal & Afaque Quraishi & Shailesh Kumar Jadhav, 2019. "Bioethanol production from Madhuca latifolia L. flowers by a newly isolated strain of Pichia kudriavzevii," Energy & Environment, , vol. 30(8), pages 1477-1490, December.
    9. Behera, Shuvashish & Arora, Richa & Nandhagopal, N. & Kumar, Sachin, 2014. "Importance of chemical pretreatment for bioconversion of lignocellulosic biomass," Renewable and Sustainable Energy Reviews, Elsevier, vol. 36(C), pages 91-106.
    10. Mishra, Abhishek & Sharma, Ajay K. & Sharma, Sumit & Bagai, Rashmi & Mathur, Anshu S. & Gupta, Ravi P. & Tuli, Deepak K., 2016. "Lignocellulosic ethanol production employing immobilized Saccharomyces cerevisiae in packed bed reactor," Renewable Energy, Elsevier, vol. 98(C), pages 57-63.
    11. Sermyagina, Ekaterina & Saari, Jussi & Zakeri, Behnam & Kaikko, Juha & Vakkilainen, Esa, 2015. "Effect of heat integration method and torrefaction temperature on the performance of an integrated CHP-torrefaction plant," Applied Energy, Elsevier, vol. 149(C), pages 24-34.
    12. Chen, Wei-Hsin & Tu, Yi-Jian & Sheen, Herng-Kuang, 2011. "Disruption of sugarcane bagasse lignocellulosic structure by means of dilute sulfuric acid pretreatment with microwave-assisted heating," Applied Energy, Elsevier, vol. 88(8), pages 2726-2734, August.
    13. He, Jie & Zhang, Wennan, 2011. "Techno-economic evaluation of thermo-chemical biomass-to-ethanol," Applied Energy, Elsevier, vol. 88(4), pages 1224-1232, April.
    14. Rattanapan, Anuchit & Limtong, Savitree & Phisalaphong, Muenduen, 2011. "Ethanol production by repeated batch and continuous fermentations of blackstrap molasses using immobilized yeast cells on thin-shell silk cocoons," Applied Energy, Elsevier, vol. 88(12), pages 4400-4404.
    15. Moretti, Marcia Maria de Souza & Bocchini-Martins, Daniela Alonso & Nunes, Christiane da Costa Carreira & Villena, Maria Arévalo & Perrone, Olavo Micali & Silva, Roberto da & Boscolo, Maurício & Gomes, 2014. "Pretreatment of sugarcane bagasse with microwaves irradiation and its effects on the structure and on enzymatic hydrolysis," Applied Energy, Elsevier, vol. 122(C), pages 189-195.
    16. Sermyagina, Ekaterina & Saari, Jussi & Kaikko, Juha & Vakkilainen, Esa, 2016. "Integration of torrefaction and CHP plant: Operational and economic analysis," Applied Energy, Elsevier, vol. 183(C), pages 88-99.
    17. Kohl, Thomas & Laukkanen, Timo & Järvinen, Mika & Fogelholm, Carl-Johan, 2013. "Energetic and environmental performance of three biomass upgrading processes integrated with a CHP plant," Applied Energy, Elsevier, vol. 107(C), pages 124-134.
    18. Karagoz, Pınar & Bill, Roslyn M. & Ozkan, Melek, 2019. "Lignocellulosic ethanol production: Evaluation of new approaches, cell immobilization and reactor configurations," Renewable Energy, Elsevier, vol. 143(C), pages 741-752.
    19. Kyriakou, Maria & Chatziiona, Vasiliki K. & Costa, Costas N. & Kallis, Michalis & Koutsokeras, Loukas & Constantinides, Georgios & Koutinas, Michalis, 2019. "Biowaste-based biochar: A new strategy for fermentative bioethanol overproduction via whole-cell immobilization," Applied Energy, Elsevier, vol. 242(C), pages 480-491.
    20. Shafiei, Marzieh & Zilouei, Hamid & Zamani, Akram & Taherzadeh, Mohammad J. & Karimi, Keikhosro, 2013. "Enhancement of ethanol production from spruce wood chips by ionic liquid pretreatment," Applied Energy, Elsevier, vol. 102(C), pages 163-169.

    Corrections

    All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:eee:appene:v:100:y:2012:i:c:p:19-26. See general information about how to correct material in RePEc.

    If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.

    If CitEc recognized a bibliographic reference but did not link an item in RePEc to it, you can help with this form .

    If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.

    For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: Catherine Liu (email available below). General contact details of provider: http://www.elsevier.com/wps/find/journaldescription.cws_home/405891/description#description .

    Please note that corrections may take a couple of weeks to filter through the various RePEc services.

    IDEAS is a RePEc service. RePEc uses bibliographic data supplied by the respective publishers.