IDEAS home Printed from https://ideas.repec.org/a/nat/natcom/v15y2024i1d10.1038_s41467-024-54843-5.html
   My bibliography  Save this article

Collagen signaling and matrix stiffness regulate multipotency in glandular epithelial stem cells in mice

Author

Listed:
  • Chen Jiang

    (Université Libre de Bruxelles (ULB))

  • Alessia Centonze

    (Université Libre de Bruxelles (ULB))

  • Yura Song

    (Université Libre de Bruxelles (ULB))

  • Antonius Chrisnandy

    (Ecole Polytechnique Fédérale de Lausanne (EPFL))

  • Elisavet Tika

    (Université Libre de Bruxelles (ULB))

  • Saba Rezakhani

    (Ecole Polytechnique Fédérale de Lausanne (EPFL))

  • Zahra Zahedi

    (Université Libre de Bruxelles (ULB))

  • Gaëlle Bouvencourt

    (Université Libre de Bruxelles (ULB))

  • Christine Dubois

    (Université Libre de Bruxelles (ULB))

  • Alexandra Van Keymeulen

    (Université Libre de Bruxelles (ULB))

  • Matthias Lütolf

    (Ecole Polytechnique Fédérale de Lausanne (EPFL)
    School of Basic Science (SB), EPFL
    Pharma Research and Early Development (pRED), F. Hoffmann-La Roche Ltd)

  • Alejandro Sifrim

    (University of Leuven, KU Leuven)

  • Cédric Blanpain

    (Université Libre de Bruxelles (ULB)
    Université Libre de Bruxelles (ULB))

Abstract

Glandular epithelia, including mammary gland (MG) and prostate, are composed of luminal and basal cells. During embryonic development, glandular epithelia arise from multipotent stem cells (SCs) that are replaced after birth by unipotent basal and unipotent luminal SCs. Different conditions, such as basal cell transplantation, luminal cell ablation, and oncogene expression can reinduce adult basal SC (BaSCs) multipotency in different glandular epithelia. The mechanisms regulating the reactivation of multipotency are incompletely understood. Here, we have found that Collagen I expression is commonly upregulated in BaSCs across the different multipotent conditions. Increasing collagen concentration or stiffness of the extracellular matrix (ECM) promotes BaSC multipotency in MG and prostate organoids. Single cell RNA-seq of MG organoids in stiff conditions have uncovered the importance of β1 integrin/FAK/AP-1 axis in the regulation of BaSC multipotency. Altogether our study uncovers the key role of Collagen signaling and ECM stiffness in the regulation of multipotency in glandular epithelia.

Suggested Citation

  • Chen Jiang & Alessia Centonze & Yura Song & Antonius Chrisnandy & Elisavet Tika & Saba Rezakhani & Zahra Zahedi & Gaëlle Bouvencourt & Christine Dubois & Alexandra Van Keymeulen & Matthias Lütolf & Al, 2024. "Collagen signaling and matrix stiffness regulate multipotency in glandular epithelial stem cells in mice," Nature Communications, Nature, vol. 15(1), pages 1-19, December.
    • Handle: RePEc:nat:natcom:v:15:y:2024:i:1:d:10.1038_s41467-024-54843-5
    DOI: 10.1038/s41467-024-54843-5
    as

    Download full text from publisher

    File URL: https://www.nature.com/articles/s41467-024-54843-5
    File Function: Abstract
    Download Restriction: no
    File URL: https://libkey.io/10.1038/s41467-024-54843-5?utm_source=ideas
    LibKey link: if access is restricted and if your library uses this service, LibKey will redirect you to where you can use your library subscription to access this item
    ---><---

    More about this item

    Statistics

    Access and download statistics

    Corrections

    All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:nat:natcom:v:15:y:2024:i:1:d:10.1038_s41467-024-54843-5. See general information about how to correct material in RePEc.

    If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.

    We have no bibliographic references for this item. You can help adding them by using this form .

    If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.

    For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: Sonal Shukla or Springer Nature Abstracting and Indexing (email available below). General contact details of provider: http://www.nature.com .

    Please note that corrections may take a couple of weeks to filter through the various RePEc services.

    IDEAS is a RePEc service. RePEc uses bibliographic data supplied by the respective publishers.