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Programmable integrin and N-cadherin adhesive interactions modulate mechanosensing of mesenchymal stem cells by cofilin phosphorylation

Author

Listed:
  • Zheng Zhang

    (Xi’an Jiaotong University
    Xi’an Jiaotong University)

  • Baoyong Sha

    (Xi’an Medical University)

  • Lingzhu Zhao

    (Xi’an Jiaotong University
    Xi’an Jiaotong University)

  • Huan Zhang

    (Xi’an Jiaotong University
    Xi’an Jiaotong University)

  • Jinteng Feng

    (Xi’an Jiaotong University
    Xi’an Jiaotong University
    First Affiliated Hospital of Xi’an Jiaotong University)

  • Cheng Zhang

    (Xi’an Jiaotong University
    Xi’an Jiaotong University)

  • Lin Sun

    (Xi’an Jiaotong University
    Xi’an Jiaotong University)

  • Meiqing Luo

    (Xi’an Jiaotong University
    Xi’an Jiaotong University)

  • Bin Gao

    (Second Affiliated Hospital of Air Force Military Medical University)

  • Hui Guo

    (First Affiliated Hospital of Xi’an Jiaotong University)

  • Zheng Wang

    (First Affiliated Hospital of Xi’an Jiaotong University)

  • Feng Xu

    (Xi’an Jiaotong University
    Xi’an Jiaotong University)

  • Tian Jian Lu

    (Nanjing University of Aeronautics and Astronautics
    Nanjing University of Aeronautics and Astronautics)

  • Guy M. Genin

    (Xi’an Jiaotong University
    Xi’an Jiaotong University
    Washington University in St. Louis
    Washington University in St. Louis)

  • Min Lin

    (Xi’an Jiaotong University
    Xi’an Jiaotong University)

Abstract

During mesenchymal development, the sources of mechanical forces transduced by cells transition over time from predominantly cell-cell interactions to predominantly cell-extracellular matrix (ECM) interactions. Transduction of the associated mechanical signals is critical for development, but how these signals converge to regulate human mesenchymal stem cells (hMSCs) mechanosensing is not fully understood, in part because time-evolving mechanical signals cannot readily be presented in vitro. Here, we established a DNA-driven cell culture platform that could be programmed to present the RGD peptide from fibronectin, mimicking cell-ECM interactions, and the HAVDI peptide from N-cadherin, mimicking cell-cell interactions, through DNA hybridization and toehold-mediated strand displacement reactions. The platform could be programmed to mimic the evolving cell-ECM and cell-cell interactions during mesenchymal development. We applied this platform to reveal that RGD/integrin ligation promoted cofilin phosphorylation, while HAVDI/N-cadherin ligation inhibited cofilin phosphorylation. Cofilin phosphorylation upregulated perinuclear apical actin fibers, which deformed the nucleus and thereby induced YAP nuclear localization in hMSCs, resulting in subsequent osteogenic differentiation. Our programmable culture platform is broadly applicable to the study of dynamic, integrated mechanobiological signals in development, healing, and tissue engineering.

Suggested Citation

  • Zheng Zhang & Baoyong Sha & Lingzhu Zhao & Huan Zhang & Jinteng Feng & Cheng Zhang & Lin Sun & Meiqing Luo & Bin Gao & Hui Guo & Zheng Wang & Feng Xu & Tian Jian Lu & Guy M. Genin & Min Lin, 2022. "Programmable integrin and N-cadherin adhesive interactions modulate mechanosensing of mesenchymal stem cells by cofilin phosphorylation," Nature Communications, Nature, vol. 13(1), pages 1-14, December.
  • Handle: RePEc:nat:natcom:v:13:y:2022:i:1:d:10.1038_s41467-022-34424-0
    DOI: 10.1038/s41467-022-34424-0
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    References listed on IDEAS

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