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Cloning of the wheat leaf rust resistance gene Lr47 introgressed from Aegilops speltoides

Author

Listed:
  • Hongna Li

    (Peking University Institute of Advanced Agricultural Sciences, Shandong Laboratory of Advanced Agricultural Sciences in Weifang)

  • Lei Hua

    (Peking University Institute of Advanced Agricultural Sciences, Shandong Laboratory of Advanced Agricultural Sciences in Weifang)

  • Shuqing Zhao

    (Hebei Agricultural University)

  • Ming Hao

    (Sichuan Agricultural University)

  • Rui Song

    (Peking University Institute of Advanced Agricultural Sciences, Shandong Laboratory of Advanced Agricultural Sciences in Weifang)

  • Shuyong Pang

    (Peking University Institute of Advanced Agricultural Sciences, Shandong Laboratory of Advanced Agricultural Sciences in Weifang
    Hebei Agricultural University)

  • Yanna Liu

    (Peking University Institute of Advanced Agricultural Sciences, Shandong Laboratory of Advanced Agricultural Sciences in Weifang)

  • Hong Chen

    (Sichuan Agricultural University)

  • Wenjun Zhang

    (University of California)

  • Tao Shen

    (Peking University Institute of Advanced Agricultural Sciences, Shandong Laboratory of Advanced Agricultural Sciences in Weifang)

  • Jin-Ying Gou

    (China Agricultural University)

  • Hailiang Mao

    (Huazhong Agricultural University)

  • Guiping Wang

    (Peking University Institute of Advanced Agricultural Sciences, Shandong Laboratory of Advanced Agricultural Sciences in Weifang)

  • Xiaohua Hao

    (Peking University Institute of Advanced Agricultural Sciences, Shandong Laboratory of Advanced Agricultural Sciences in Weifang)

  • Jian Li

    (Peking University Institute of Advanced Agricultural Sciences, Shandong Laboratory of Advanced Agricultural Sciences in Weifang)

  • Baoxing Song

    (Peking University Institute of Advanced Agricultural Sciences, Shandong Laboratory of Advanced Agricultural Sciences in Weifang)

  • Caixia Lan

    (Huazhong Agricultural University)

  • Zaifeng Li

    (Hebei Agricultural University)

  • Xing Wang Deng

    (Peking University Institute of Advanced Agricultural Sciences, Shandong Laboratory of Advanced Agricultural Sciences in Weifang)

  • Jorge Dubcovsky

    (University of California
    Howard Hughes Medical Institute)

  • Xiaodong Wang

    (Hebei Agricultural University)

  • Shisheng Chen

    (Peking University Institute of Advanced Agricultural Sciences, Shandong Laboratory of Advanced Agricultural Sciences in Weifang)

Abstract

Leaf rust, caused by Puccinia triticina Eriksson (Pt), is one of the most severe foliar diseases of wheat. Breeding for leaf rust resistance is a practical and sustainable method to control this devastating disease. Here, we report the identification of Lr47, a broadly effective leaf rust resistance gene introgressed into wheat from Aegilops speltoides. Lr47 encodes a coiled-coil nucleotide-binding leucine-rich repeat protein that is both necessary and sufficient to confer Pt resistance, as demonstrated by loss-of-function mutations and transgenic complementation. Lr47 introgression lines with no or reduced linkage drag are generated using the Pairing homoeologous1 mutation, and a diagnostic molecular marker for Lr47 is developed. The coiled-coil domain of the Lr47 protein is unable to induce cell death, nor does it have self-protein interaction. The cloning of Lr47 expands the number of leaf rust resistance genes that can be incorporated into multigene transgenic cassettes to control this devastating disease.

Suggested Citation

  • Hongna Li & Lei Hua & Shuqing Zhao & Ming Hao & Rui Song & Shuyong Pang & Yanna Liu & Hong Chen & Wenjun Zhang & Tao Shen & Jin-Ying Gou & Hailiang Mao & Guiping Wang & Xiaohua Hao & Jian Li & Baoxing, 2023. "Cloning of the wheat leaf rust resistance gene Lr47 introgressed from Aegilops speltoides," Nature Communications, Nature, vol. 14(1), pages 1-15, December.
    • Handle: RePEc:nat:natcom:v:14:y:2023:i:1:d:10.1038_s41467-023-41833-2
    DOI: 10.1038/s41467-023-41833-2
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    References listed on IDEAS

    as
    1. Xiaopeng Ren & Chuyuan Wang & Zhuang Ren & Jing Wang & Peipei Zhang & Shuqing Zhao & Mengyu Li & Meng Yuan & Xiumei Yu & Zaifeng Li & Shisheng Chen & Xiaodong Wang, 2023. "Genetics of Resistance to Leaf Rust in Wheat: An Overview in a Genome-Wide Level," Sustainability, MDPI, vol. 15(4), pages 1-27, February.
    2. Guifang Lin & Hui Chen & Bin Tian & Sunish K. Sehgal & Lovepreet Singh & Jingzhong Xie & Nidhi Rawat & Philomin Juliana & Narinder Singh & Sandesh Shrestha & Duane L. Wilson & Hannah Shult & Hyeonju L, 2022. "Cloning of the broadly effective wheat leaf rust resistance gene Lr42 transferred from Aegilops tauschii," Nature Communications, Nature, vol. 13(1), pages 1-12, December.
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    Cited by:

    1. Chao Ma & Xiubin Tian & Zhenjie Dong & Huanhuan Li & Xuexue Chen & Wenxuan Liu & Guihong Yin & Shuyang Ma & Liwei Zhang & Aizhong Cao & Cheng Liu & Hongfei Yan & Sunish K. Sehgal & Zhibin Zhang & Bao , 2024. "An Aegilops longissima NLR protein with integrated CC-BED module mediates resistance to wheat powdery mildew," Nature Communications, Nature, vol. 15(1), pages 1-13, December.
    2. Huagang He & Zhaozhao Chen & Renchun Fan & Jie Zhang & Shanying Zhu & Jiale Wang & Qianyuan Zhang & Anli Gao & Shuangjun Gong & Lu Zhang & Yanan Li & Yitong Zhao & Simon G. Krattinger & Qian-Hua Shen , 2024. "A kinase fusion protein from Aegilops longissima confers resistance to wheat powdery mildew," Nature Communications, Nature, vol. 15(1), pages 1-13, December.
    3. Huanhuan Li & Wenqiang Men & Chao Ma & Qianwen Liu & Zhenjie Dong & Xiubin Tian & Chaoli Wang & Cheng Liu & Harsimardeep S. Gill & Pengtao Ma & Zhibin Zhang & Bao Liu & Yue Zhao & Sunish K. Sehgal & W, 2024. "Wheat powdery mildew resistance gene Pm13 encodes a mixed lineage kinase domain-like protein," Nature Communications, Nature, vol. 15(1), pages 1-13, December.

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